ID   PRS46_RAT               Reviewed;         314 AA.
AC   Q6IE63;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2012, sequence version 2.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Serine protease 46;
DE            EC=3.4.21.-;
DE   AltName: Full=Testis-specific serine protease-6;
GN   Name=Prss46; Synonyms=Tessp6;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 1-230.
RA   Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RC   STRAIN=Sprague-Dawley;
RX   PubMed=15060002; DOI=10.1101/gr.1946304;
RA   Puente X.S., Lopez-Otin C.;
RT   "A genomic analysis of rat proteases and protease inhibitors.";
RL   Genome Res. 14:609-622(2004).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00274}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CH473984; EDL77052.1; -; Genomic_DNA.
DR   EMBL; BN000330; CAE48385.1; -; mRNA.
DR   RefSeq; NP_001008865.1; NM_001008865.1.
DR   RefSeq; XP_006244055.1; XM_006243993.2.
DR   AlphaFoldDB; Q6IE63; -.
DR   SMR; Q6IE63; -.
DR   STRING; 10116.ENSRNOP00000028479; -.
DR   MEROPS; S01.250; -.
DR   PaxDb; Q6IE63; -.
DR   Ensembl; ENSRNOT00000028479; ENSRNOP00000028479; ENSRNOG00000020981.
DR   GeneID; 408245; -.
DR   KEGG; rno:408245; -.
DR   UCSC; RGD:1302970; rat.
DR   CTD; 74306; -.
DR   RGD; 1302970; Prss46.
DR   eggNOG; KOG3627; Eukaryota.
DR   GeneTree; ENSGT00940000162940; -.
DR   HOGENOM; CLU_006842_0_4_1; -.
DR   InParanoid; Q6IE63; -.
DR   OMA; EICNHRY; -.
DR   OrthoDB; 1314811at2759; -.
DR   PRO; PR:Q6IE63; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   Proteomes; UP000234681; Chromosome 5.
DR   Bgee; ENSRNOG00000020981; Expressed in testis and 2 other tissues.
DR   Genevisible; Q6IE63; RN.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR   GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 1.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Hydrolase; Membrane; Protease; Reference proteome;
KW   Serine protease; Transmembrane; Transmembrane helix.
FT   CHAIN           1..314
FT                   /note="Serine protease 46"
FT                   /id="PRO_0000418334"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          44..281
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        84
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        130
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        233
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   DISULFID        69..85
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        164..239
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        197..219
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        229..257
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ   SEQUENCE   314 AA;  34999 MW;  722ECD51D8C930E5 CRC64;
     MACGSVDPQG LLSPPLSSAR LSNVPHVEGP WFWSCGQTNI SCKVVNGKVV EVGKWPWQVS
     ILFLGMYICS GSLIHHHWVL TAAHCLQRSK NPANYTVKVG VQTLPDNSSS ELLVTSIVIH
     ENFINHMSHD IAILKLKYPV TWSPFIQPIC LPEVNFKPSI GTMCWVIGWG LEKAKGAPKT
     PYSVQGVAVR IVNNEICNHR YQFLLLKNQK KFIGNDMLCT SPEWGLDTCQ DASGSSLVCQ
     MNKTWIQMGV VSWNFGCGRR QFPSIYTSTS HFTQWIKRQI GDLKFASMAV PSFLSPFILT
     GYILLVSLGS LWLL