PRS52_MOUSE
ID PRS52_MOUSE Reviewed; 321 AA.
AC Q9D9M0; A8C1Y0; Q80Y38;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Serine protease 52;
DE EC=3.4.21.-;
DE AltName: Full=Testicular-specific serine protease 3;
DE Flags: Precursor;
GN Name=Prss52; Synonyms=Tesp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Baba T., Kohno N., Sato S.;
RT "Structural characterization of testicular specific serine protease,
RT TESP3.";
RL Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable serine protease. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB24725.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB009660; BAF80445.1; -; mRNA.
DR EMBL; AK006746; BAB24725.1; ALT_INIT; mRNA.
DR EMBL; BC049714; AAH49714.1; -; mRNA.
DR CCDS; CCDS36953.1; -.
DR RefSeq; NP_082801.2; NM_028525.2.
DR AlphaFoldDB; Q9D9M0; -.
DR SMR; Q9D9M0; -.
DR STRING; 10090.ENSMUSP00000022537; -.
DR MEROPS; S01.419; -.
DR GlyGen; Q9D9M0; 1 site.
DR iPTMnet; Q9D9M0; -.
DR PhosphoSitePlus; Q9D9M0; -.
DR PaxDb; Q9D9M0; -.
DR PRIDE; Q9D9M0; -.
DR ProteomicsDB; 291803; -.
DR DNASU; 73382; -.
DR Ensembl; ENSMUST00000022537; ENSMUSP00000022537; ENSMUSG00000021966.
DR GeneID; 73382; -.
DR KEGG; mmu:73382; -.
DR UCSC; uc007uie.1; mouse.
DR CTD; 73382; -.
DR MGI; MGI:1920632; Prss52.
DR VEuPathDB; HostDB:ENSMUSG00000021966; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT00940000154999; -.
DR HOGENOM; CLU_006842_0_4_1; -.
DR InParanoid; Q9D9M0; -.
DR OMA; DIIDFPW; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q9D9M0; -.
DR TreeFam; TF351676; -.
DR BioGRID-ORCS; 73382; 0 hits in 73 CRISPR screens.
DR PRO; PR:Q9D9M0; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q9D9M0; protein.
DR Bgee; ENSMUSG00000021966; Expressed in seminiferous tubule of testis and 11 other tissues.
DR Genevisible; Q9D9M0; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 1.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR InterPro; IPR033116; TRYPSIN_SER.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
DR PROSITE; PS00135; TRYPSIN_SER; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Membrane; Protease;
KW Reference proteome; Serine protease; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..321
FT /note="Serine protease 52"
FT /id="PRO_0000328817"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 56..287
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 96
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 142
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 236
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 153
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 81..97
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 175..242
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 208..221
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 232..263
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT CONFLICT 49
FT /note="E -> G (in Ref. 1; BAF80445)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 321 AA; 35883 MW; B57615886B65E393 CRC64;
MKRWKDRRTG LLLPLVLLLF GACSSLAWVC GRRMSSRSQQ LNNASAIVEG KPASAIVGGK
PANILEFPWH VGIMNHGSHL CGGSILNEWW VLSASHCFDQ LNNSKLEIIH GTEDLSTKGI
KYQKVDKLFL HPKFDDWLLD NDIALLLLKS PLNLSVNRIP ICTSEISDIQ AWRNCWVTGW
GITNTSEKGV QPTILQAVKV DLYRWDWCGY ILSLLTKNML CAGTQDPGKD ACQGDSGGAL
VCNKKRNTAI WYQVGIVSWG MGCGKKNLPG VYTKVSHYVR WISKQTAKAG RPYMYEQNSA
CPLVLSCRAI LFLYFVMFLL T
