PRS54_HUMAN
ID PRS54_HUMAN Reviewed; 395 AA.
AC Q6PEW0; Q96LN9; Q9NT77;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Inactive serine protease 54;
DE AltName: Full=Cancer/testis antigen 67;
DE Short=CT67;
DE AltName: Full=Plasma kallikrein-like protein 4;
DE Flags: Precursor;
GN Name=PRSS54; Synonyms=KLKBL4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS GLY-182; ILE-240 AND
RP ALA-295.
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 106-395.
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. Plasma kallikrein
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU00274}.
CC -!- CAUTION: Although related to peptidase S1 family, lacks the essential
CC His, Asp, and Ser residues of the catalytic triad at positions 83, 129
CC and 221 and is therefore predicted to have lost protease activity.
CC {ECO:0000305}.
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DR EMBL; AK058068; BAB71649.1; -; mRNA.
DR EMBL; BC057843; AAH57843.2; -; mRNA.
DR EMBL; AL137484; CAB70765.1; -; mRNA.
DR CCDS; CCDS32463.1; -.
DR PIR; T46470; T46470.
DR RefSeq; NP_001073961.1; NM_001080492.1.
DR RefSeq; NP_001292102.1; NM_001305173.1.
DR AlphaFoldDB; Q6PEW0; -.
DR SMR; Q6PEW0; -.
DR STRING; 9606.ENSP00000219301; -.
DR MEROPS; S01.992; -.
DR GlyGen; Q6PEW0; 2 sites.
DR BioMuta; PRSS54; -.
DR DMDM; 148876818; -.
DR MassIVE; Q6PEW0; -.
DR PaxDb; Q6PEW0; -.
DR PeptideAtlas; Q6PEW0; -.
DR PRIDE; Q6PEW0; -.
DR ProteomicsDB; 67082; -.
DR Antibodypedia; 29072; 84 antibodies from 15 providers.
DR DNASU; 221191; -.
DR Ensembl; ENST00000219301.8; ENSP00000219301.4; ENSG00000103023.12.
DR Ensembl; ENST00000567164.6; ENSP00000455024.1; ENSG00000103023.12.
DR GeneID; 221191; -.
DR KEGG; hsa:221191; -.
DR MANE-Select; ENST00000567164.6; ENSP00000455024.1; NM_001305173.2; NP_001292102.1.
DR UCSC; uc002enf.3; human.
DR CTD; 221191; -.
DR DisGeNET; 221191; -.
DR GeneCards; PRSS54; -.
DR HGNC; HGNC:26336; PRSS54.
DR HPA; ENSG00000103023; Tissue enriched (testis).
DR neXtProt; NX_Q6PEW0; -.
DR OpenTargets; ENSG00000103023; -.
DR PharmGKB; PA165450675; -.
DR VEuPathDB; HostDB:ENSG00000103023; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT01020000230389; -.
DR HOGENOM; CLU_058835_0_0_1; -.
DR InParanoid; Q6PEW0; -.
DR OMA; LVQPICF; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q6PEW0; -.
DR TreeFam; TF338267; -.
DR PathwayCommons; Q6PEW0; -.
DR BioGRID-ORCS; 221191; 13 hits in 1069 CRISPR screens.
DR GenomeRNAi; 221191; -.
DR Pharos; Q6PEW0; Tdark.
DR PRO; PR:Q6PEW0; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q6PEW0; protein.
DR Bgee; ENSG00000103023; Expressed in left testis and 62 other tissues.
DR ExpressionAtlas; Q6PEW0; baseline and differential.
DR Genevisible; Q6PEW0; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001254; Trypsin_dom.
DR Pfam; PF00089; Trypsin; 1.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Serine protease homolog; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..395
FT /note="Inactive serine protease 54"
FT /id="PRO_0000288801"
FT DOMAIN 37..269
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT REGION 324..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..345
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 327
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 164..227
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 195..205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 217..248
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT VARIANT 182
FT /note="S -> G (in dbSNP:rs3815803)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_032497"
FT VARIANT 240
FT /note="V -> I (in dbSNP:rs1052276)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_032498"
FT VARIANT 295
FT /note="T -> A (in dbSNP:rs2241414)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_032499"
SQ SEQUENCE 395 AA; 43832 MW; 5414B2DB041066EF CRC64;
MVSAAGLSGD GKMRGVLLVL LGLLYSSTSC GVQKASVFYG PDPKEGLVSS MEFPWVVSLQ
DSQYTHLAFG CILSEFWVLS IASAIQNRKD IVVIVGISNM DPSKIAHTEY PVNTIIIHED
FDNNSMSNNI ALLKTDTAMH FGNLVQSICF LGRMLHTPPV LQNCWVSGWN PTSATGNHMT
MSVLRKIFVK DLDMCPLYKL QKTECGSHTK EETKTACLGD PGSPMMCQLQ QFDLWVLRGV
LNFGGETCPG LFLYTKVEDY SKWITSKAER AGPPLSSLHH WEKLISFSHH GPNATMTQKT
YSDSELGHVG SYLQGQRRTI THSRLGNSSR DSLDVREKDV KESGRSPEAS VQPLYYDYYG
GEVGEGRIFA GQNRLYQPEE IILVSFVLVF FCSSI
