PRS58_HUMAN
ID PRS58_HUMAN Reviewed; 241 AA.
AC Q8IYP2; B3KVJ6; D3DXD2;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Serine protease 58;
DE EC=3.4.21.4;
DE AltName: Full=Trypsin-X3;
DE Flags: Precursor;
GN Name=PRSS58; Synonyms=TRY1, TRYX3; ORFNames=UNQ2540/PRO6090;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=16751668; DOI=10.1534/genetics.106.055715;
RA Bubb K.L., Bovee D., Buckley D., Haugen E., Kibukawa M., Paddock M.,
RA Palmieri A., Subramanian S., Zhou Y., Kaul R., Green P., Olson M.V.;
RT "Scan of human genome reveals no new loci under ancient balancing
RT selection.";
RL Genetics 173:2165-2177(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.; EC=3.4.21.4;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase S1 family. {ECO:0000255|PROSITE-
CC ProRule:PRU00274}.
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DR EMBL; AK122940; BAG53808.1; -; mRNA.
DR EMBL; DQ384426; ABD48876.1; -; Genomic_DNA.
DR EMBL; DQ384427; ABD48877.1; -; Genomic_DNA.
DR EMBL; DQ384428; ABD48878.1; -; Genomic_DNA.
DR EMBL; DQ384429; ABD48879.1; -; Genomic_DNA.
DR EMBL; DQ384431; ABD48880.1; -; Genomic_DNA.
DR EMBL; AY358487; AAQ88851.1; -; mRNA.
DR EMBL; CH236959; EAL23772.1; -; Genomic_DNA.
DR EMBL; CH471198; EAW51980.1; -; Genomic_DNA.
DR EMBL; CH471198; EAW51981.1; -; Genomic_DNA.
DR EMBL; BC035384; AAH35384.1; -; mRNA.
DR CCDS; CCDS5871.1; -.
DR RefSeq; NP_001001317.1; NM_001001317.4.
DR AlphaFoldDB; Q8IYP2; -.
DR SMR; Q8IYP2; -.
DR BioGRID; 126461; 1.
DR STRING; 9606.ENSP00000446916; -.
DR MEROPS; S01.085; -.
DR GlyGen; Q8IYP2; 2 sites.
DR iPTMnet; Q8IYP2; -.
DR PhosphoSitePlus; Q8IYP2; -.
DR BioMuta; PRSS58; -.
DR DMDM; 74759717; -.
DR MassIVE; Q8IYP2; -.
DR PaxDb; Q8IYP2; -.
DR PeptideAtlas; Q8IYP2; -.
DR PRIDE; Q8IYP2; -.
DR ProteomicsDB; 71211; -.
DR Antibodypedia; 50270; 32 antibodies from 9 providers.
DR DNASU; 136541; -.
DR Ensembl; ENST00000547058.6; ENSP00000447588.2; ENSG00000258223.6.
DR Ensembl; ENST00000552471.1; ENSP00000446916.1; ENSG00000258223.6.
DR Ensembl; ENST00000621886.4; ENSP00000478535.1; ENSG00000276785.4.
DR Ensembl; ENST00000622880.1; ENSP00000479165.1; ENSG00000276785.4.
DR GeneID; 136541; -.
DR KEGG; hsa:136541; -.
DR MANE-Select; ENST00000547058.6; ENSP00000447588.2; NM_001001317.5; NP_001001317.1.
DR UCSC; uc003vxb.4; human.
DR CTD; 136541; -.
DR DisGeNET; 136541; -.
DR GeneCards; PRSS58; -.
DR HGNC; HGNC:39125; PRSS58.
DR HPA; ENSG00000258223; Tissue enriched (testis).
DR neXtProt; NX_Q8IYP2; -.
DR OpenTargets; ENSG00000258223; -.
DR VEuPathDB; HostDB:ENSG00000258223; -.
DR eggNOG; KOG3627; Eukaryota.
DR GeneTree; ENSGT01020000230389; -.
DR HOGENOM; CLU_006842_1_6_1; -.
DR InParanoid; Q8IYP2; -.
DR OMA; RWCGGSI; -.
DR OrthoDB; 1314811at2759; -.
DR PhylomeDB; Q8IYP2; -.
DR TreeFam; TF331065; -.
DR PathwayCommons; Q8IYP2; -.
DR BioGRID-ORCS; 136541; 11 hits in 1063 CRISPR screens.
DR GenomeRNAi; 136541; -.
DR Pharos; Q8IYP2; Tdark.
DR PRO; PR:Q8IYP2; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q8IYP2; protein.
DR Bgee; ENSG00000258223; Expressed in left testis and 16 other tissues.
DR Genevisible; Q8IYP2; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030141; C:secretory granule; IBA:GO_Central.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00190; Tryp_SPc; 1.
DR Gene3D; 2.40.10.10; -; 2.
DR InterPro; IPR009003; Peptidase_S1_PA.
DR InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR InterPro; IPR001314; Peptidase_S1A.
DR InterPro; IPR001254; Trypsin_dom.
DR InterPro; IPR018114; TRYPSIN_HIS.
DR Pfam; PF00089; Trypsin; 1.
DR PRINTS; PR00722; CHYMOTRYPSIN.
DR SMART; SM00020; Tryp_SPc; 1.
DR SUPFAM; SSF50494; SSF50494; 1.
DR PROSITE; PS50240; TRYPSIN_DOM; 1.
DR PROSITE; PS00134; TRYPSIN_HIS; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW Secreted; Serine protease; Signal.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..241
FT /note="Serine protease 58"
FT /id="PRO_0000317763"
FT DOMAIN 18..239
FT /note="Peptidase S1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT ACT_SITE 56
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 101
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 195
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 173
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 41..57
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 133..201
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 165..180
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT DISULFID 191..215
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
SQ SEQUENCE 241 AA; 27085 MW; 964829EE2BAD60AA CRC64;
MKFILLWALL NLTVALAFNP DYTVSSTPPY LVYLKSDYLP CAGVLIHPLW VITAAHCNLP
KLRVILGVTI PADSNEKHLQ VIGYEKMIHH PHFSVTSIDH DIMLIKLKTE AELNDYVKLA
NLPYQTISEN TMCSVSTWSY NVCDIYKEPD SLQTVNISVI SKPQCRDAYK TYNITENMLC
VGIVPGRRQP CKEVSAAPAI CNGMLQGILS FADGCVLRAD VGIYAKIFYY IPWIENVIQN
N
