PRSA2_BACAN
ID PRSA2_BACAN Reviewed; 285 AA.
AC Q81TU1; Q6I224; Q6KVW2;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Foldase protein PrsA 2;
DE EC=5.2.1.8;
DE Flags: Precursor;
GN Name=prsA2; Synonyms=prsA-2; OrderedLocusNames=BA_1169, GBAA_1169, BAS1084;
OS Bacillus anthracis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=1392;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames / isolate Porton;
RX PubMed=12721629; DOI=10.1038/nature01586;
RA Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T.,
RA Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R.,
RA Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M.,
RA Kolonay J.F., Beanan M.J., Dodson R.J., Brinkac L.M., Gwinn M.L.,
RA DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C.,
RA Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y.,
RA Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M.,
RA Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E.,
RA White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M.,
RA Hanna P.C., Kolstoe A.-B., Fraser C.M.;
RT "The genome sequence of Bacillus anthracis Ames and comparison to closely
RT related bacteria.";
RL Nature 423:81-86(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ames ancestor;
RX PubMed=18952800; DOI=10.1128/jb.01347-08;
RA Ravel J., Jiang L., Stanley S.T., Wilson M.R., Decker R.S., Read T.D.,
RA Worsham P., Keim P.S., Salzberg S.L., Fraser-Liggett C.M., Rasko D.A.;
RT "The complete genome sequence of Bacillus anthracis Ames 'Ancestor'.";
RL J. Bacteriol. 191:445-446(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Sterne;
RA Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA Richardson P., Rubin E., Tice H.;
RT "Complete genome sequence of Bacillus anthracis Sterne.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP CHARACTERIZATION.
RX PubMed=12606539; DOI=10.1074/jbc.m301244200;
RA Williams R.C., Rees M.L., Jacobs M.F., Pragai Z., Thwaite J.E.,
RA Baillie L.W., Emmerson P.T., Harwood C.R.;
RT "Production of Bacillus anthracis protective antigen is dependent on the
RT extracellular chaperone, PrsA.";
RL J. Biol. Chem. 278:18056-18062(2003).
CC -!- FUNCTION: Plays a major role in protein secretion by helping the post-
CC translocational extracellular folding of several secreted proteins.
CC Important for the secretion of the protective antigen. The three PsrA
CC proteins in this organism show different but overlapping substrate
CC specificities.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-peptidylproline (omega=180) = [protein]-
CC peptidylproline (omega=0); Xref=Rhea:RHEA:16237, Rhea:RHEA-
CC COMP:10747, Rhea:RHEA-COMP:10748, ChEBI:CHEBI:83833,
CC ChEBI:CHEBI:83834; EC=5.2.1.8;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PrsA family. {ECO:0000305}.
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DR EMBL; AE016879; AAP25134.1; -; Genomic_DNA.
DR EMBL; AE017334; AAT30258.1; -; Genomic_DNA.
DR EMBL; AE017225; AAT53407.1; -; Genomic_DNA.
DR RefSeq; NP_843648.1; NC_003997.3.
DR RefSeq; WP_001214195.1; NZ_WXXJ01000044.1.
DR RefSeq; YP_027356.1; NC_005945.1.
DR PDB; 6VJ4; X-ray; 1.70 A; A=29-285.
DR PDBsum; 6VJ4; -.
DR AlphaFoldDB; Q81TU1; -.
DR SMR; Q81TU1; -.
DR STRING; 261594.GBAA_1169; -.
DR DNASU; 1089182; -.
DR EnsemblBacteria; AAP25134; AAP25134; BA_1169.
DR EnsemblBacteria; AAT30258; AAT30258; GBAA_1169.
DR GeneID; 45021180; -.
DR KEGG; ban:BA_1169; -.
DR KEGG; bar:GBAA_1169; -.
DR KEGG; bat:BAS1084; -.
DR PATRIC; fig|198094.11.peg.1148; -.
DR eggNOG; COG0760; Bacteria.
DR HOGENOM; CLU_034646_6_1_9; -.
DR OMA; QKAKNQY; -.
DR Proteomes; UP000000427; Chromosome.
DR Proteomes; UP000000594; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006457; P:protein folding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.40; -; 1.
DR HAMAP; MF_01145; Foldase_PrsA; 1.
DR InterPro; IPR023059; Foldase_PrsA.
DR InterPro; IPR046357; PPIase_dom_sf.
DR InterPro; IPR000297; PPIase_PpiC.
DR InterPro; IPR023058; PPIase_PpiC_CS.
DR InterPro; IPR027304; Trigger_fact/SurA_dom_sf.
DR Pfam; PF00639; Rotamase; 1.
DR SUPFAM; SSF109998; SSF109998; 1.
DR PROSITE; PS01096; PPIC_PPIASE_1; 1.
DR PROSITE; PS50198; PPIC_PPIASE_2; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Isomerase; Lipoprotein; Membrane; Palmitate;
KW Reference proteome; Rotamase; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..285
FT /note="Foldase protein PrsA 2"
FT /id="PRO_0000029292"
FT DOMAIN 134..224
FT /note="PpiC"
FT LIPID 21
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 21
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255"
FT HELIX 55..68
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 73..87
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 88..90
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 91..97
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 103..122
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 126..131
FT /evidence="ECO:0007829|PDB:6VJ4"
FT STRAND 137..145
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 147..158
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 163..170
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 174..177
FT /evidence="ECO:0007829|PDB:6VJ4"
FT TURN 178..181
FT /evidence="ECO:0007829|PDB:6VJ4"
FT STRAND 182..186
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 193..201
FT /evidence="ECO:0007829|PDB:6VJ4"
FT STRAND 211..213
FT /evidence="ECO:0007829|PDB:6VJ4"
FT STRAND 216..225
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 231..247
FT /evidence="ECO:0007829|PDB:6VJ4"
FT HELIX 250..263
FT /evidence="ECO:0007829|PDB:6VJ4"
SQ SEQUENCE 285 AA; 32079 MW; D328DD7C3B6CAB5B CRC64;
MRGKHIFIIT ALISILMLAA CGQKNSSATV ATATDSTITK SDFEKQLKDR YGKDMLYEMI
AQDVITKKYK VSDDDVDKEV QKAKSQYGDQ FKNVLKNNGL KDEADFKNQI KFKLSMNKAI
KQSVTEKDVK DHYKPEIKAS HILVSDENEA KEIKKKLDTG ASFEELAKQE SQDLLSKEKG
GDLGYFHSGA MTPEFETAAY KLKIGQISDP VQSPNGYHII KLTGKKDLKP YDEVKNSIRK
NLEEERTADP IFGKKLLQSE LKKANIKIND SELEDTFTIV SPQGN
