ID   AAC32_SALSP             Reviewed;         286 AA.
AC   P0A255; P13245;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Aminoglycoside N(3)-acetyltransferase III;
DE            EC=2.3.1.81;
DE   AltName: Full=ACC(3)-III;
DE   AltName: Full=Aminocyclitol 3-N-acetyltransferase type III;
DE   AltName: Full=Gentamicin-(3)-N-acetyl-transferase;
GN   Name=aacC3; Synonyms=aac3-VA, aacC2;
OS   Salmonella sp.
OG   Plasmid pWP116a, and Plasmid pWP14a.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=599;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pWP116a, and pWP14a;
RX   PubMed=3892230; DOI=10.1007/bf00332949;
RA   Allmansberger R., Braeu B., Piepersberg W.;
RT   "Genes for gentamicin-(3)-N-acetyl-transferases III and IV. II. Nucleotide
RT   sequences of three AAC(3)-III genes and evolutionary aspects.";
RL   Mol. Gen. Genet. 198:514-520(1985).
CC   -!- FUNCTION: Resistance to antibiotics containing the 2-deoxy-streptamine
CC       ring including gentamicin, kanamycin, tobramycin, neomycin and
CC       apramycin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2-deoxystreptamine antibiotic + acetyl-CoA = an N(3')-
CC         acetyl-2-deoxystreptamine antibiotic + CoA + H(+);
CC         Xref=Rhea:RHEA:12665, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57921, ChEBI:CHEBI:77452; EC=2.3.1.81;
CC   -!- SIMILARITY: Belongs to the antibiotic N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X13541; CAA31891.1; -; Genomic_DNA.
DR   EMBL; X13542; CAA31893.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A255; -.
DR   SMR; P0A255; -.
DR   GO; GO:0046353; F:aminoglycoside 3-N-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003679; Amioglycoside_AcTrfase.
DR   InterPro; IPR028345; Antibiotic_NAT-like.
DR   PANTHER; PTHR11104; PTHR11104; 1.
DR   Pfam; PF02522; Antibiotic_NAT; 1.
DR   SUPFAM; SSF110710; SSF110710; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Plasmid; Transferase.
FT   CHAIN           1..286
FT                   /note="Aminoglycoside N(3)-acetyltransferase III"
FT                   /id="PRO_0000068544"
SQ   SEQUENCE   286 AA;  30609 MW;  AE30E9D093CBBD1F CRC64;
     MHTRKAITEA IRKLGVQTGD LLMVHASLKA IGPVEGGAET VVAALRSAVG PTGTVMGYAS
     WDRSPYEETL NGARLDDKAR RTWPPFDPAT AGTYRGFGLL NQFLVQAPGA RRSAHPDASM
     VAVGPLAETL TEPHELGHAL GEGSPVERFV RLGGKALLLG APLNSVTALH YAEAVADIPN
     KRWVTYEMPM LGRNGEVAWK TASEYDSNGI LDCFAIEGKP DAVETIANAY VKLGRHREGV
     VGFAQCYLFD AQDIVTFGVT YLEKHFGATP IVPAHEAAQR SCEPSG