ID   ATG3_BOVIN              Reviewed;         314 AA.
AC   Q0VCL3;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG3;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 3;
DE            Short=APG3-like;
GN   Name=ATG3;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal pons;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E2-like enzyme involved in autophagy and mitochondrial
CC       homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP,
CC       GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-
CC       conjugation to ATG8-like proteins is essential for autophagy. Preferred
CC       substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme,
CC       catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to
CC       ATG3 playing a role in mitochondrial homeostasis but not in autophagy.
CC       ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2
CC       complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from
CC       centriolar satellites via the autophagic pathway (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3
CC       and ATG7, plays a role in the conjugation of ATG12 to ATG5. Interacts
CC       with FNBP1L (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in
CC       regulation of mitochondrial homeostasis and cell death, while it is not
CC       involved in PE-conjugation to ATG8-like proteins and autophagy (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG3 family. {ECO:0000305}.
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DR   EMBL; BC120110; AAI20111.1; -; mRNA.
DR   RefSeq; NP_001068832.1; NM_001075364.1.
DR   AlphaFoldDB; Q0VCL3; -.
DR   SMR; Q0VCL3; -.
DR   STRING; 9913.ENSBTAP00000011976; -.
DR   PaxDb; Q0VCL3; -.
DR   PeptideAtlas; Q0VCL3; -.
DR   PRIDE; Q0VCL3; -.
DR   Ensembl; ENSBTAT00000011976; ENSBTAP00000011976; ENSBTAG00000009084.
DR   GeneID; 508571; -.
DR   KEGG; bta:508571; -.
DR   CTD; 64422; -.
DR   VEuPathDB; HostDB:ENSBTAG00000009084; -.
DR   VGNC; VGNC:26256; ATG3.
DR   eggNOG; KOG2981; Eukaryota.
DR   GeneTree; ENSGT00390000010308; -.
DR   HOGENOM; CLU_027518_0_0_1; -.
DR   InParanoid; Q0VCL3; -.
DR   OMA; YDKYYQV; -.
DR   OrthoDB; 1432328at2759; -.
DR   TreeFam; TF105903; -.
DR   Proteomes; UP000009136; Chromosome 1.
DR   Bgee; ENSBTAG00000009084; Expressed in monocyte and 104 other tissues.
DR   ExpressionAtlas; Q0VCL3; baseline and differential.
DR   GO; GO:0000153; C:cytoplasmic ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0019777; F:Atg12 transferase activity; ISS:UniProtKB.
DR   GO; GO:0019776; F:Atg8 ligase activity; ISS:UniProtKB.
DR   GO; GO:0019899; F:enzyme binding; IEA:Ensembl.
DR   GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR   GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR   GO; GO:0043653; P:mitochondrial fragmentation involved in apoptotic process; ISS:UniProtKB.
DR   GO; GO:0050765; P:negative regulation of phagocytosis; IEA:Ensembl.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:Ensembl.
DR   GO; GO:1902017; P:regulation of cilium assembly; ISS:UniProtKB.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   PANTHER; PTHR12866; PTHR12866; 1.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Autophagy; Cytoplasm; Isopeptide bond; Protein transport;
KW   Reference proteome; Transferase; Transport; Ubl conjugation;
KW   Ubl conjugation pathway.
FT   CHAIN           1..314
FT                   /note="Ubiquitin-like-conjugating enzyme ATG3"
FT                   /id="PRO_0000286938"
FT   REGION          143..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        264
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NT62"
FT   CROSSLNK        243
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ATG12)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  35752 MW;  206F95C2D8FB99FD CRC64;
     MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE
     LKVKAYLPSG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGIAGIT
     EAVKEITLES KDSIKLQDCS ALCEEEEEED EGEAADMEEY EESGLLETDE ATLDTRKIVE
     ACKAKTDAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH
     VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV
     IPTIEYDYTR HFTM