ID   ATG3_DANRE              Reviewed;         317 AA.
AC   Q6PFS7;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Ubiquitin-like-conjugating enzyme ATG3;
DE            EC=2.3.2.-;
DE   AltName: Full=Autophagy-related protein 3;
DE            Short=APG3-like;
GN   Name=atg3; Synonyms=apg3l; ORFNames=zgc:64156;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: E2 conjugating enzyme required for the cytoplasm to vacuole
CC       transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible
CC       for the E2-like covalent binding of phosphatidylethanolamine to the C-
CC       terminal Gly of atg8-like proteins (gabarap, gabarapl1, gabarapl2 or
CC       map1lc3a). The atg12-atg5 conjugate plays a role of an E3 and promotes
CC       the transfer of atg8-like proteins from atg3 to
CC       phosphatidylethanolamine (PE). This step is required for the membrane
CC       association of atg8-like proteins. The formation of the atg8-
CC       phosphatidylethanolamine conjugates is essential for autophagy and for
CC       the cytoplasm to vacuole transport (Cvt). Also acts as an autocatalytic
CC       E2-like enzyme, catalyzing the conjugation of atg12 to itself, atg12
CC       conjugation to atg3 playing a role in mitochondrial homeostasis but not
CC       in autophagy. atg7 (E1-like enzyme) facilitates this reaction by
CC       forming an E1-E2 complex with atg3 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with atg7 and atg12. The complex composed of atg3
CC       and atg7 plays a role in the conjugation of atg12 to atg5.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- PTM: Conjugated to atg12 at Lys-246. ATG12-conjugation plays a role in
CC       regulation of mitochondrial homeostasis and cell death, while it is not
CC       involved in PE-conjugation to ATG8-like proteins and autophagy (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG3 family. {ECO:0000305}.
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DR   EMBL; BC057434; AAH57434.1; -; mRNA.
DR   RefSeq; NP_956316.1; NM_200022.1.
DR   AlphaFoldDB; Q6PFS7; -.
DR   SMR; Q6PFS7; -.
DR   STRING; 7955.ENSDARP00000041303; -.
DR   PaxDb; Q6PFS7; -.
DR   DNASU; 336632; -.
DR   Ensembl; ENSDART00000041304; ENSDARP00000041303; ENSDARG00000009350.
DR   GeneID; 336632; -.
DR   KEGG; dre:336632; -.
DR   CTD; 64422; -.
DR   ZFIN; ZDB-GENE-030131-8576; atg3.
DR   eggNOG; KOG2981; Eukaryota.
DR   GeneTree; ENSGT00390000010308; -.
DR   HOGENOM; CLU_027518_0_0_1; -.
DR   InParanoid; Q6PFS7; -.
DR   OMA; YDKYYQV; -.
DR   OrthoDB; 1432328at2759; -.
DR   PhylomeDB; Q6PFS7; -.
DR   TreeFam; TF105903; -.
DR   Reactome; R-DRE-1632852; Macroautophagy.
DR   PRO; PR:Q6PFS7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 6.
DR   Bgee; ENSDARG00000009350; Expressed in spleen and 30 other tissues.
DR   GO; GO:0000153; C:cytoplasmic ubiquitin ligase complex; IBA:GO_Central.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0019777; F:Atg12 transferase activity; ISS:UniProtKB.
DR   GO; GO:0019776; F:Atg8 ligase activity; ISS:UniProtKB.
DR   GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR   GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR   GO; GO:0043653; P:mitochondrial fragmentation involved in apoptotic process; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007135; Atg3/Atg10.
DR   PANTHER; PTHR12866; PTHR12866; 1.
DR   Pfam; PF03987; Autophagy_act_C; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Cytoplasm; Isopeptide bond; Protein transport;
KW   Reference proteome; Transferase; Transport; Ubl conjugation;
KW   Ubl conjugation pathway.
FT   CHAIN           1..317
FT                   /note="Ubiquitin-like-conjugating enzyme ATG3"
FT                   /id="PRO_0000213572"
FT   REGION          137..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        147..165
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        267
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   CROSSLNK        246
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ATG12)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   317 AA;  35699 MW;  2FC8CD5FCC5639BC CRC64;
     MQNVINSVKG TALGVAEFLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWKWASGEE
     AKVKPYLPND KQFLLTRNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT FHNSGVTGVT
     EAVREISLDN KDNMNMNVKT GACGNSGDDD DDEEGEAADM EEYEESGLLE TDDATLDTSK
     MADLSKTKAE AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEDRQPL TVDQMYEDIS
     QDHVKKTVTI ENHPNLPPPA MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV
     QAVIPTIEYD YTRHFTM