ID   PRT1_SEPOF              Reviewed;          78 AA.
AC   P80001;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 2.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Spermatid-specific protein T1;
DE   Contains:
DE     RecName: Full=Spermatid-specific protein T1B;
DE   Contains:
DE     RecName: Full=Sperm protamine SP1;
OS   Sepia officinalis (Common cuttlefish).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda;
OC   Coleoidea; Decapodiformes; Sepiida; Sepiina; Sepiidae; Sepia.
OX   NCBI_TaxID=6610;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=1894625; DOI=10.1016/s0021-9258(19)47385-7;
RA   Wouters-Tyrou D., Chartier-Harlin M.-C., Martin-Ponthieu A., Boutillon C.,
RA   van Dorsselaer A., Sautiere P.;
RT   "Cuttlefish spermatid-specific protein T. Molecular characterization of two
RT   variants T1 and T2, putative precursors of sperm protamine variants Sp1 and
RT   Sp2.";
RL   J. Biol. Chem. 266:17388-17395(1991).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-78.
RX   PubMed=1999185; DOI=10.1111/j.1432-1033.1991.tb15744.x;
RA   Martin-Ponthieu A., Wouters-Tyrou D., Belaiche D., Sautiere P.,
RA   Schindler P., van Dorsselaer A.;
RT   "Cuttlefish sperm protamines. 1. Amino acid sequences of two distinct
RT   variants.";
RL   Eur. J. Biochem. 195:611-619(1991).
CC   -!- FUNCTION: Cuttlefish spermiogenesis is characterized by a double
CC       nuclear protein transition: histones -> spermatid-specific proteins
CC       (T1/T2) -> protamines (SP1/SP2). The protamines compact sperm DNA into
CC       a highly condensed, stable and inactive complex.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- DEVELOPMENTAL STAGE: Spermiogenesis.
CC   -!- PTM: Phosphorylation occurs at different degrees. The triphosphorylated
CC       form may be predominant in T1. SP1 appears to be phosphorylated in
CC       elongated spermatids, but dephosphorylated in mature sperm cells.
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DR   PIR; A40973; A40973.
DR   AlphaFoldDB; P80001; -.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Chromosome; Developmental protein; Differentiation;
KW   Direct protein sequencing; DNA condensation; DNA-binding; Nucleosome core;
KW   Nucleus; Phosphoprotein; Spermatogenesis.
FT   CHAIN           1..78
FT                   /note="Spermatid-specific protein T1"
FT                   /id="PRO_0000025823"
FT   CHAIN           2..78
FT                   /note="Spermatid-specific protein T1B"
FT                   /id="PRO_0000045874"
FT   CHAIN           22..78
FT                   /note="Sperm protamine SP1"
FT                   /id="PRO_0000025824"
FT   REGION          1..78
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1..21
FT                   /note="Hydrophobic"
FT   COMPBIAS        20..78
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   78 AA;  10632 MW;  09FE3EDBF0DCED33 CRC64;
     MKVAANSSKM LAEKLELMKG GRRRRRRSRR RRRRSRRRSR SPYRRRYRRR RRRRRRRSRR
     RRYRRRRSYS RRRYRRRR