ATG3_DICDI
ID ATG3_DICDI Reviewed; 338 AA.
AC Q550A8; Q86K79;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 3.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Autophagy-related protein 3;
GN Name=atg3; Synonyms=apg3; ORFNames=DDB_G0277319;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: E2 conjugating enzyme required for the cytoplasm to vacuole
CC transport (Cvt), and autophagy. Responsible for the E2-like covalent
CC binding of phosphatidylethanolamine to the C-terminal Gly of atg8. The
CC atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of
CC atg8 from atg3 to phosphatidylethanolamine (PE). This step is required
CC for the membrane association of atg8. The formation of the atg8-
CC phosphatidylethanolamine conjugates is essential for autophagy and for
CC the cytoplasm to vacuole transport (Cvt) (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with atg7 and atg12. The complex composed of atg3
CC and atg7 plays a role in the conjugation of atg12 to atg5.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- PTM: Conjugated to atg12. atg12-conjugation plays a role in regulation
CC of mitochondrial homeostasis and cell death, while it is not involved
CC in PE-conjugation to atg8 and autophagy (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG3 family. {ECO:0000305}.
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DR EMBL; AAFI02000019; EAL68846.2; -; Genomic_DNA.
DR RefSeq; XP_642708.2; XM_637616.2.
DR AlphaFoldDB; Q550A8; -.
DR SMR; Q550A8; -.
DR BioGRID; 1245903; 1.
DR STRING; 44689.DDB0252605; -.
DR PaxDb; Q550A8; -.
DR EnsemblProtists; EAL68846; EAL68846; DDB_G0277319.
DR GeneID; 8620900; -.
DR KEGG; ddi:DDB_G0277319; -.
DR dictyBase; DDB_G0277319; atg3.
DR eggNOG; KOG2981; Eukaryota.
DR HOGENOM; CLU_027518_0_0_1; -.
DR InParanoid; Q550A8; -.
DR OMA; YDKYYQV; -.
DR PhylomeDB; Q550A8; -.
DR Reactome; R-DDI-1632852; Macroautophagy.
DR PRO; PR:Q550A8; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0000153; C:cytoplasmic ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019776; F:Atg8 ligase activity; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR007135; Atg3/Atg10.
DR PANTHER; PTHR12866; PTHR12866; 1.
DR Pfam; PF03987; Autophagy_act_C; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasm; Protein transport; Reference proteome; Transport;
KW Ubl conjugation; Ubl conjugation pathway.
FT CHAIN 1..338
FT /note="Autophagy-related protein 3"
FT /id="PRO_0000327427"
FT REGION 91..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..155
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 157..183
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 288
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 338 AA; 38817 MW; B1A58603638F10CC CRC64;
MLTSFQQAVH KAYVKTVEKV TPTLSTSKFL EEGVLTPEEF VQAGDLLTDK CQTWTWESGD
PSRNVSYLPK EKQFLLTRNV PCYNRVRTLE NESKASKADE IQIEDDGEDS WVAPQPVGNQ
DDIEDEKVDI SSLKIDDKKP NTTTTTTAKP TNNNNNNNDD EDEDEDGDIP DLDDFQDDNI
IEEEDPAVLS KNNKTTTTTT ANNNNNNNSE NKVEDNDNIL RTRTYDISIT YDKYYQTPRV
WLFGYDENRK PLKPEEIFED ISEDHAHKTV TIDSHPHLGI SFAYIHPCRH AAVMKKLVDR
QSENGKEPRV DQYLFLFLKF ISVVIPTIEY DFTLEFDT
