PRTK_PARAQ
ID PRTK_PARAQ Reviewed; 384 AA.
AC P06873;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Proteinase K;
DE EC=3.4.21.64;
DE AltName: Full=Endopeptidase K;
DE AltName: Full=Tritirachium alkaline proteinase;
DE Flags: Precursor;
GN Name=PROK;
OS Parengyodontium album (Tritirachium album).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Cordycipitaceae; Parengyodontium.
OX NCBI_TaxID=37998;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=ATCC 22563 / Limber;
RX PubMed=2645134; DOI=10.1111/j.1432-1033.1989.tb14539.x;
RA Gunkel F.A., Gassen H.G.;
RT "Proteinase K from Tritirachium album Limber. Characterization of the
RT chromosomal gene and expression of the cDNA in Escherichia coli.";
RL Eur. J. Biochem. 179:185-194(1989).
RN [2]
RP PROTEIN SEQUENCE OF 106-384.
RA Jany K.-D., Lederer G., Mayer B.;
RT "Proteinase K - a new subclass of the subtilisins. The amino acid sequence
RT of the enzyme.";
RL Biol. Chem. Hoppe-Seyler 367:87-87(1986).
RN [3]
RP PROTEIN SEQUENCE OF 106-384.
RA Jany K.-D., Lederer G., Mayer B.;
RT "Amino acid sequence of proteinase K from the mold Tritirachium album
RT limber. Proteinase K - a subtilisin-related enzyme with disulfide bonds.";
RL FEBS Lett. 199:139-144(1986).
RN [4]
RP PROTEIN SEQUENCE OF 259-343.
RX PubMed=3924077; DOI=10.1515/bchm3.1985.366.1.485;
RA Jany K.-D., Mayer B.;
RT "Proteinase K from Tritirachium album limber. I. Molecular mass and
RT sequence around the active site serine residue.";
RL Biol. Chem. Hoppe-Seyler 366:485-492(1985).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS).
RX PubMed=6378621; DOI=10.1002/j.1460-2075.1984.tb01968.x;
RA Paehler A., Banerjee A., Dattagupta J.K., Fujiwara T., Lindner K.,
RA Pal G.P., Suck D., Weber G., Saenger W.;
RT "Three-dimensional structure of fungal proteinase K reveals similarity to
RT bacterial subtilisin.";
RL EMBO J. 3:1311-1314(1984).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX PubMed=8083213; DOI=10.2210/pdb2pkc/pdb;
RA Mueller A., Hinrichs W., Wolf W.M., Saenger W.;
RT "Crystal structure of calcium-free proteinase K at 1.5-A resolution.";
RL J. Biol. Chem. 269:23108-23111(1994).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.44 ANGSTROMS).
RX PubMed=9741842;
RX DOI=10.1002/(sici)1097-0134(19981001)33:1<30::aid-prot3>3.0.co;2-p;
RA Singh T.P., Sharma S., Karthikeyan S., Betzel C., Bhatia K.L.;
RT "Crystal structure of a complex formed between proteolytically-generated
RT lactoferrin fragment and proteinase K.";
RL Proteins 33:30-38(1998).
CC -!- FUNCTION: Hydrolyzes keratin at aromatic and hydrophobic residues.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of keratin, and of other proteins with subtilisin-
CC like specificity. Hydrolyzes peptide amides.; EC=3.4.21.64;
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Note=Binds 2 calcium ions per subunit.;
CC -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Worthington enzyme manual;
CC URL="https://www.worthington-biochem.com/PROK/";
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DR EMBL; X14689; CAA32820.1; -; Genomic_DNA.
DR EMBL; X14688; CAA32819.1; -; mRNA.
DR PIR; S02142; SUTIKA.
DR PDB; 1BJR; X-ray; 2.44 A; E=106-384.
DR PDB; 1CNM; X-ray; 2.20 A; A=106-384.
DR PDB; 1EGQ; X-ray; 1.55 A; A=106-384.
DR PDB; 1HT3; X-ray; 1.80 A; A=106-384.
DR PDB; 1IC6; X-ray; 0.98 A; A=106-384.
DR PDB; 1OYO; X-ray; 2.02 A; A=106-384.
DR PDB; 1P7V; X-ray; 1.08 A; A=106-384.
DR PDB; 1P7W; X-ray; 1.02 A; A=106-384.
DR PDB; 1PEK; X-ray; 2.20 A; E=106-384.
DR PDB; 1PFG; X-ray; 2.50 A; A=106-384.
DR PDB; 1PJ8; X-ray; 2.20 A; A=106-384.
DR PDB; 1PTK; X-ray; 2.40 A; A=106-384.
DR PDB; 2DP4; X-ray; 2.90 A; E=106-384.
DR PDB; 2DQK; X-ray; 1.93 A; A=106-384.
DR PDB; 2DUJ; X-ray; 1.67 A; A=106-384.
DR PDB; 2G4V; X-ray; 2.14 A; A=106-384.
DR PDB; 2HD4; X-ray; 2.15 A; A=106-384.
DR PDB; 2HPZ; X-ray; 1.69 A; A=106-384.
DR PDB; 2ID8; X-ray; 1.27 A; A=106-384.
DR PDB; 2PKC; X-ray; 1.50 A; A=106-384.
DR PDB; 2PQ2; X-ray; 1.82 A; A=106-384.
DR PDB; 2PRK; X-ray; 1.50 A; A=106-384.
DR PDB; 2PWA; X-ray; 0.83 A; A=106-384.
DR PDB; 2PWB; X-ray; 1.90 A; A=106-384.
DR PDB; 2PYZ; X-ray; 1.79 A; A=106-384.
DR PDB; 2V8B; X-ray; 0.94 A; A=106-384.
DR PDB; 3AJ8; X-ray; 1.10 A; A=106-384.
DR PDB; 3AJ9; X-ray; 1.10 A; A=106-384.
DR PDB; 3D9Q; X-ray; 1.43 A; X=106-384.
DR PDB; 3DDZ; X-ray; 1.70 A; X=106-384.
DR PDB; 3DE0; X-ray; 1.90 A; X=106-384.
DR PDB; 3DE1; X-ray; 2.00 A; X=106-384.
DR PDB; 3DE2; X-ray; 2.10 A; X=106-384.
DR PDB; 3DE3; X-ray; 1.43 A; X=106-384.
DR PDB; 3DE4; X-ray; 1.80 A; X=106-384.
DR PDB; 3DE5; X-ray; 2.10 A; X=106-384.
DR PDB; 3DE6; X-ray; 2.20 A; X=106-384.
DR PDB; 3DE7; X-ray; 2.30 A; X=106-384.
DR PDB; 3DVQ; X-ray; 1.02 A; X=106-384.
DR PDB; 3DVR; X-ray; 1.02 A; X=106-384.
DR PDB; 3DVS; X-ray; 1.02 A; X=106-384.
DR PDB; 3DW1; X-ray; 1.03 A; X=106-384.
DR PDB; 3DW3; X-ray; 0.99 A; X=106-384.
DR PDB; 3DWE; X-ray; 0.99 A; X=106-384.
DR PDB; 3DYB; X-ray; 1.32 A; A=106-384.
DR PDB; 3GT3; X-ray; 1.50 A; A=106-384.
DR PDB; 3GT4; X-ray; 1.76 A; A=106-384.
DR PDB; 3I2Y; X-ray; 1.00 A; X=106-384.
DR PDB; 3I30; X-ray; 0.99 A; X=106-384.
DR PDB; 3I34; X-ray; 1.00 A; X=106-384.
DR PDB; 3I37; X-ray; 1.00 A; X=106-384.
DR PDB; 3L1K; X-ray; 1.55 A; A=106-384.
DR PDB; 3OSZ; X-ray; 2.26 A; A=106-384.
DR PDB; 3PRK; X-ray; 2.20 A; E=106-384.
DR PDB; 3PTL; X-ray; 1.30 A; A=106-384.
DR PDB; 3Q40; X-ray; 1.80 A; A=106-384.
DR PDB; 3Q5G; X-ray; 1.77 A; A=106-384.
DR PDB; 3QMP; X-ray; 1.10 A; A=106-384.
DR PDB; 4B5L; X-ray; 1.60 A; A=106-384.
DR PDB; 4DJ5; X-ray; 1.80 A; X=106-384.
DR PDB; 4FON; X-ray; 1.05 A; A=106-384.
DR PDB; 4WOB; X-ray; 1.90 A; A=106-384.
DR PDB; 4WOC; X-ray; 1.60 A; A=106-384.
DR PDB; 4ZAR; X-ray; 1.15 A; A=106-384.
DR PDB; 5AMX; X-ray; 1.01 A; A=106-384.
DR PDB; 5AVJ; X-ray; 1.45 A; A=106-384.
DR PDB; 5AVK; X-ray; 1.45 A; A=106-384.
DR PDB; 5B1D; X-ray; 2.30 A; A=106-384.
DR PDB; 5B1E; X-ray; 2.30 A; A=106-384.
DR PDB; 5CW1; X-ray; 1.45 A; A=106-384.
DR PDB; 5I9S; EM; 1.75 A; A=106-384.
DR PDB; 5K7S; EM; 1.60 A; A=106-384.
DR PDB; 5KXU; X-ray; 1.20 A; A=106-384.
DR PDB; 5KXV; X-ray; 0.98 A; A=106-384.
DR PDB; 5MJL; X-ray; 2.21 A; A=106-384.
DR PDB; 5ROC; X-ray; 1.52 A; A=106-384.
DR PDB; 5ROD; X-ray; 1.04 A; A=106-384.
DR PDB; 5ROE; X-ray; 1.50 A; A=106-384.
DR PDB; 5ROF; X-ray; 1.08 A; A=106-384.
DR PDB; 5ROG; X-ray; 1.08 A; A=106-384.
DR PDB; 5ROH; X-ray; 1.04 A; A=106-384.
DR PDB; 5ROI; X-ray; 1.05 A; A=106-384.
DR PDB; 5ROJ; X-ray; 1.02 A; A=106-384.
DR PDB; 5ROK; X-ray; 1.04 A; A=106-384.
DR PDB; 5ROL; X-ray; 1.22 A; A=106-384.
DR PDB; 5ROM; X-ray; 1.07 A; A=106-384.
DR PDB; 5RON; X-ray; 1.22 A; A=106-384.
DR PDB; 5ROO; X-ray; 1.41 A; A=106-384.
DR PDB; 5ROP; X-ray; 1.64 A; A=106-384.
DR PDB; 5ROQ; X-ray; 1.02 A; A=106-384.
DR PDB; 5ROR; X-ray; 1.22 A; A=106-384.
DR PDB; 5ROS; X-ray; 1.45 A; A=106-384.
DR PDB; 5ROT; X-ray; 1.05 A; A=106-384.
DR PDB; 5ROU; X-ray; 1.06 A; A=106-384.
DR PDB; 5ROV; X-ray; 1.04 A; A=106-384.
DR PDB; 5ROW; X-ray; 1.21 A; A=106-384.
DR PDB; 5ROX; X-ray; 1.12 A; A=106-384.
DR PDB; 5ROY; X-ray; 1.05 A; A=106-384.
DR PDB; 5ROZ; X-ray; 1.14 A; A=106-384.
DR PDB; 5RP0; X-ray; 1.03 A; A=106-384.
DR PDB; 5RP1; X-ray; 1.11 A; A=106-384.
DR PDB; 5RP2; X-ray; 1.10 A; A=106-384.
DR PDB; 5RP3; X-ray; 1.09 A; A=106-384.
DR PDB; 5RP4; X-ray; 1.05 A; A=106-384.
DR PDB; 5RP5; X-ray; 1.09 A; A=106-384.
DR PDB; 5RP6; X-ray; 1.17 A; A=106-384.
DR PDB; 5RP7; X-ray; 1.15 A; A=106-384.
DR PDB; 5RP8; X-ray; 1.03 A; A=106-384.
DR PDB; 5RP9; X-ray; 1.48 A; A=106-384.
DR PDB; 5RPA; X-ray; 1.25 A; A=106-384.
DR PDB; 5RPB; X-ray; 1.64 A; A=106-384.
DR PDB; 5RPC; X-ray; 1.23 A; A=106-384.
DR PDB; 5RPD; X-ray; 1.02 A; A=106-384.
DR PDB; 5RPE; X-ray; 1.02 A; A=106-384.
DR PDB; 5RPF; X-ray; 1.39 A; A=106-384.
DR PDB; 5RPG; X-ray; 1.50 A; A=106-384.
DR PDB; 5RPH; X-ray; 1.38 A; A=106-384.
DR PDB; 5RPI; X-ray; 1.03 A; A=106-384.
DR PDB; 5RPJ; X-ray; 1.27 A; A=106-384.
DR PDB; 5RPK; X-ray; 1.78 A; A=106-384.
DR PDB; 5RPL; X-ray; 1.09 A; A=106-384.
DR PDB; 5RPM; X-ray; 1.22 A; A=106-384.
DR PDB; 5RPN; X-ray; 1.02 A; A=106-384.
DR PDB; 5RPO; X-ray; 1.50 A; A=106-384.
DR PDB; 5RPP; X-ray; 1.08 A; A=106-384.
DR PDB; 5RPQ; X-ray; 1.07 A; A=106-384.
DR PDB; 5RPR; X-ray; 1.16 A; A=106-384.
DR PDB; 5RPS; X-ray; 1.04 A; A=106-384.
DR PDB; 5RPT; X-ray; 1.98 A; A=106-384.
DR PDB; 5RPU; X-ray; 1.55 A; A=106-384.
DR PDB; 5RPV; X-ray; 1.10 A; A=106-384.
DR PDB; 5RPW; X-ray; 1.02 A; A=106-384.
DR PDB; 5RPX; X-ray; 1.05 A; A=106-384.
DR PDB; 5RPY; X-ray; 1.09 A; A=106-384.
DR PDB; 5RPZ; X-ray; 1.22 A; A=106-384.
DR PDB; 5UVL; X-ray; 2.65 A; A=106-384.
DR PDB; 5WHW; X-ray; 1.71 A; A=106-384.
DR PDB; 5WJG; X-ray; 1.50 A; A=106-384.
DR PDB; 5WJH; X-ray; 1.63 A; A=106-384.
DR PDB; 5WRC; X-ray; 1.50 A; A=106-384.
DR PDB; 6CL7; EM; 1.71 A; A=106-384.
DR PDB; 6CL8; EM; 2.00 A; A=106-384.
DR PDB; 6CL9; EM; 2.20 A; A=106-384.
DR PDB; 6CLA; EM; 2.80 A; A=106-384.
DR PDB; 6CLB; EM; 3.20 A; A=106-384.
DR PDB; 6FJS; X-ray; 1.90 A; A=106-384.
DR PDB; 6J43; X-ray; 1.85 A; A=106-384.
DR PDB; 6K2P; X-ray; 1.60 A; A=106-384.
DR PDB; 6K2R; X-ray; 1.60 A; A=106-384.
DR PDB; 6K2S; X-ray; 1.60 A; A=106-384.
DR PDB; 6K2T; X-ray; 1.60 A; A=106-384.
DR PDB; 6K2V; X-ray; 1.60 A; A=106-384.
DR PDB; 6K2W; X-ray; 1.70 A; A=106-384.
DR PDB; 6K2X; X-ray; 1.65 A; A=106-384.
DR PDB; 6K8M; X-ray; 1.45 A; E=106-384.
DR PDB; 6KKF; X-ray; 1.40 A; A=106-384.
DR PDB; 6LAW; EM; 1.50 A; A=106-384.
DR PDB; 6MH6; X-ray; 1.80 A; A=106-384.
DR PDB; 6N4U; EM; 2.75 A; A=106-384.
DR PDB; 6PKJ; EM; 2.18 A; A=106-384.
DR PDB; 6PKK; EM; 2.18 A; A=106-384.
DR PDB; 6PKL; EM; 2.59 A; A=106-384.
DR PDB; 6PKM; EM; 2.17 A; A=106-384.
DR PDB; 6PKN; EM; 2.08 A; A=106-384.
DR PDB; 6PKO; EM; 2.07 A; A=106-384.
DR PDB; 6PKP; EM; 1.91 A; A=106-384.
DR PDB; 6PKQ; EM; 1.85 A; A=106-384.
DR PDB; 6PKR; EM; 1.79 A; A=106-384.
DR PDB; 6PKS; EM; 2.16 A; A=106-384.
DR PDB; 6PKT; EM; 1.85 A; A=106-384.
DR PDB; 6PQ0; EM; 2.00 A; A=106-384.
DR PDB; 6PQ4; EM; 2.00 A; A=106-384.
DR PDB; 6PU4; EM; 2.10 A; A=106-384.
DR PDB; 6PU5; EM; 2.70 A; A=106-384.
DR PDB; 6QF1; X-ray; 1.74 A; A=106-384.
DR PDB; 6QXV; X-ray; 1.94 A; A=106-384.
DR PDB; 6RUG; X-ray; 1.10 A; A=106-384.
DR PDB; 6RUH; X-ray; 1.10 A; A=106-384.
DR PDB; 6RUK; X-ray; 1.20 A; A=106-384.
DR PDB; 6RUN; X-ray; 1.10 A; A=106-384.
DR PDB; 6RUW; X-ray; 1.35 A; A=106-384.
DR PDB; 6RVE; X-ray; 1.15 A; A=106-384.
DR PDB; 6RVG; X-ray; 1.10 A; A=106-384.
DR PDB; 6RZP; X-ray; 2.20 A; A=106-384.
DR PDB; 6TXG; X-ray; 1.37 A; A=106-384.
DR PDB; 6V8R; EM; 1.60 A; A=106-384.
DR PDB; 6ZET; EM; 2.70 A; AAA=106-384.
DR PDB; 6ZEU; EM; 2.00 A; AAA=106-384.
DR PDB; 6ZEV; EM; 2.40 A; AAA=106-384.
DR PDB; 7A68; X-ray; 2.55 A; A=106-384.
DR PDB; 7A9F; X-ray; 1.62 A; A=106-384.
DR PDB; 7A9K; X-ray; 1.62 A; A=106-384.
DR PDB; 7A9M; X-ray; 1.62 A; A=106-384.
DR PDB; 7C0P; X-ray; 2.15 A; A=106-384.
DR PDB; 7JSY; EM; 1.78 A; A=106-384.
DR PDB; 7LN7; X-ray; 1.02 A; A=106-384.
DR PDB; 7LPT; X-ray; 1.43 A; A=106-384.
DR PDB; 7LPU; X-ray; 1.02 A; A=106-384.
DR PDB; 7LPV; X-ray; 1.10 A; A=106-384.
DR PDB; 7LQ8; X-ray; 1.30 A; A=106-384.
DR PDB; 7LQ9; X-ray; 1.43 A; A=106-384.
DR PDB; 7LQA; X-ray; 1.02 A; A=106-384.
DR PDB; 7LQB; X-ray; 1.02 A; A=106-384.
DR PDB; 7LQC; X-ray; 1.02 A; A=106-384.
DR PDB; 7NJJ; X-ray; 1.65 A; A=106-384.
DR PDB; 7NUY; X-ray; 1.65 A; A=106-384.
DR PDB; 7NUZ; X-ray; 1.09 A; A=106-384.
DR PDB; 7S4Z; X-ray; 1.90 A; A=106-384.
DR PDBsum; 1BJR; -.
DR PDBsum; 1CNM; -.
DR PDBsum; 1EGQ; -.
DR PDBsum; 1HT3; -.
DR PDBsum; 1IC6; -.
DR PDBsum; 1OYO; -.
DR PDBsum; 1P7V; -.
DR PDBsum; 1P7W; -.
DR PDBsum; 1PEK; -.
DR PDBsum; 1PFG; -.
DR PDBsum; 1PJ8; -.
DR PDBsum; 1PTK; -.
DR PDBsum; 2DP4; -.
DR PDBsum; 2DQK; -.
DR PDBsum; 2DUJ; -.
DR PDBsum; 2G4V; -.
DR PDBsum; 2HD4; -.
DR PDBsum; 2HPZ; -.
DR PDBsum; 2ID8; -.
DR PDBsum; 2PKC; -.
DR PDBsum; 2PQ2; -.
DR PDBsum; 2PRK; -.
DR PDBsum; 2PWA; -.
DR PDBsum; 2PWB; -.
DR PDBsum; 2PYZ; -.
DR PDBsum; 2V8B; -.
DR PDBsum; 3AJ8; -.
DR PDBsum; 3AJ9; -.
DR PDBsum; 3D9Q; -.
DR PDBsum; 3DDZ; -.
DR PDBsum; 3DE0; -.
DR PDBsum; 3DE1; -.
DR PDBsum; 3DE2; -.
DR PDBsum; 3DE3; -.
DR PDBsum; 3DE4; -.
DR PDBsum; 3DE5; -.
DR PDBsum; 3DE6; -.
DR PDBsum; 3DE7; -.
DR PDBsum; 3DVQ; -.
DR PDBsum; 3DVR; -.
DR PDBsum; 3DVS; -.
DR PDBsum; 3DW1; -.
DR PDBsum; 3DW3; -.
DR PDBsum; 3DWE; -.
DR PDBsum; 3DYB; -.
DR PDBsum; 3GT3; -.
DR PDBsum; 3GT4; -.
DR PDBsum; 3I2Y; -.
DR PDBsum; 3I30; -.
DR PDBsum; 3I34; -.
DR PDBsum; 3I37; -.
DR PDBsum; 3L1K; -.
DR PDBsum; 3OSZ; -.
DR PDBsum; 3PRK; -.
DR PDBsum; 3PTL; -.
DR PDBsum; 3Q40; -.
DR PDBsum; 3Q5G; -.
DR PDBsum; 3QMP; -.
DR PDBsum; 4B5L; -.
DR PDBsum; 4DJ5; -.
DR PDBsum; 4FON; -.
DR PDBsum; 4WOB; -.
DR PDBsum; 4WOC; -.
DR PDBsum; 4ZAR; -.
DR PDBsum; 5AMX; -.
DR PDBsum; 5AVJ; -.
DR PDBsum; 5AVK; -.
DR PDBsum; 5B1D; -.
DR PDBsum; 5B1E; -.
DR PDBsum; 5CW1; -.
DR PDBsum; 5I9S; -.
DR PDBsum; 5K7S; -.
DR PDBsum; 5KXU; -.
DR PDBsum; 5KXV; -.
DR PDBsum; 5MJL; -.
DR PDBsum; 5ROC; -.
DR PDBsum; 5ROD; -.
DR PDBsum; 5ROE; -.
DR PDBsum; 5ROF; -.
DR PDBsum; 5ROG; -.
DR PDBsum; 5ROH; -.
DR PDBsum; 5ROI; -.
DR PDBsum; 5ROJ; -.
DR PDBsum; 5ROK; -.
DR PDBsum; 5ROL; -.
DR PDBsum; 5ROM; -.
DR PDBsum; 5RON; -.
DR PDBsum; 5ROO; -.
DR PDBsum; 5ROP; -.
DR PDBsum; 5ROQ; -.
DR PDBsum; 5ROR; -.
DR PDBsum; 5ROS; -.
DR PDBsum; 5ROT; -.
DR PDBsum; 5ROU; -.
DR PDBsum; 5ROV; -.
DR PDBsum; 5ROW; -.
DR PDBsum; 5ROX; -.
DR PDBsum; 5ROY; -.
DR PDBsum; 5ROZ; -.
DR PDBsum; 5RP0; -.
DR PDBsum; 5RP1; -.
DR PDBsum; 5RP2; -.
DR PDBsum; 5RP3; -.
DR PDBsum; 5RP4; -.
DR PDBsum; 5RP5; -.
DR PDBsum; 5RP6; -.
DR PDBsum; 5RP7; -.
DR PDBsum; 5RP8; -.
DR PDBsum; 5RP9; -.
DR PDBsum; 5RPA; -.
DR PDBsum; 5RPB; -.
DR PDBsum; 5RPC; -.
DR PDBsum; 5RPD; -.
DR PDBsum; 5RPE; -.
DR PDBsum; 5RPF; -.
DR PDBsum; 5RPG; -.
DR PDBsum; 5RPH; -.
DR PDBsum; 5RPI; -.
DR PDBsum; 5RPJ; -.
DR PDBsum; 5RPK; -.
DR PDBsum; 5RPL; -.
DR PDBsum; 5RPM; -.
DR PDBsum; 5RPN; -.
DR PDBsum; 5RPO; -.
DR PDBsum; 5RPP; -.
DR PDBsum; 5RPQ; -.
DR PDBsum; 5RPR; -.
DR PDBsum; 5RPS; -.
DR PDBsum; 5RPT; -.
DR PDBsum; 5RPU; -.
DR PDBsum; 5RPV; -.
DR PDBsum; 5RPW; -.
DR PDBsum; 5RPX; -.
DR PDBsum; 5RPY; -.
DR PDBsum; 5RPZ; -.
DR PDBsum; 5UVL; -.
DR PDBsum; 5WHW; -.
DR PDBsum; 5WJG; -.
DR PDBsum; 5WJH; -.
DR PDBsum; 5WRC; -.
DR PDBsum; 6CL7; -.
DR PDBsum; 6CL8; -.
DR PDBsum; 6CL9; -.
DR PDBsum; 6CLA; -.
DR PDBsum; 6CLB; -.
DR PDBsum; 6FJS; -.
DR PDBsum; 6J43; -.
DR PDBsum; 6K2P; -.
DR PDBsum; 6K2R; -.
DR PDBsum; 6K2S; -.
DR PDBsum; 6K2T; -.
DR PDBsum; 6K2V; -.
DR PDBsum; 6K2W; -.
DR PDBsum; 6K2X; -.
DR PDBsum; 6K8M; -.
DR PDBsum; 6KKF; -.
DR PDBsum; 6LAW; -.
DR PDBsum; 6MH6; -.
DR PDBsum; 6N4U; -.
DR PDBsum; 6PKJ; -.
DR PDBsum; 6PKK; -.
DR PDBsum; 6PKL; -.
DR PDBsum; 6PKM; -.
DR PDBsum; 6PKN; -.
DR PDBsum; 6PKO; -.
DR PDBsum; 6PKP; -.
DR PDBsum; 6PKQ; -.
DR PDBsum; 6PKR; -.
DR PDBsum; 6PKS; -.
DR PDBsum; 6PKT; -.
DR PDBsum; 6PQ0; -.
DR PDBsum; 6PQ4; -.
DR PDBsum; 6PU4; -.
DR PDBsum; 6PU5; -.
DR PDBsum; 6QF1; -.
DR PDBsum; 6QXV; -.
DR PDBsum; 6RUG; -.
DR PDBsum; 6RUH; -.
DR PDBsum; 6RUK; -.
DR PDBsum; 6RUN; -.
DR PDBsum; 6RUW; -.
DR PDBsum; 6RVE; -.
DR PDBsum; 6RVG; -.
DR PDBsum; 6RZP; -.
DR PDBsum; 6TXG; -.
DR PDBsum; 6V8R; -.
DR PDBsum; 6ZET; -.
DR PDBsum; 6ZEU; -.
DR PDBsum; 6ZEV; -.
DR PDBsum; 7A68; -.
DR PDBsum; 7A9F; -.
DR PDBsum; 7A9K; -.
DR PDBsum; 7A9M; -.
DR PDBsum; 7C0P; -.
DR PDBsum; 7JSY; -.
DR PDBsum; 7LN7; -.
DR PDBsum; 7LPT; -.
DR PDBsum; 7LPU; -.
DR PDBsum; 7LPV; -.
DR PDBsum; 7LQ8; -.
DR PDBsum; 7LQ9; -.
DR PDBsum; 7LQA; -.
DR PDBsum; 7LQB; -.
DR PDBsum; 7LQC; -.
DR PDBsum; 7NJJ; -.
DR PDBsum; 7NUY; -.
DR PDBsum; 7NUZ; -.
DR PDBsum; 7S4Z; -.
DR AlphaFoldDB; P06873; -.
DR SMR; P06873; -.
DR BindingDB; P06873; -.
DR ChEMBL; CHEMBL1075070; -.
DR Allergome; 8265; Tri al Proteinase K.
DR MEROPS; S08.054; -.
DR PRIDE; P06873; -.
DR BRENDA; 3.4.21.64; 6510.
DR EvolutionaryTrace; P06873; -.
DR GO; GO:0110165; C:cellular anatomical entity; IEA:UniProt.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04077; Peptidases_S8_PCSK9_ProteinaseK_like; 1.
DR Gene3D; 3.30.70.80; -; 1.
DR Gene3D; 3.40.50.200; -; 1.
DR InterPro; IPR034193; PCSK9_ProteinaseK-like.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR010259; S8pro/Inhibitor_I9.
DR InterPro; IPR037045; S8pro/Inhibitor_I9_sf.
DR Pfam; PF05922; Inhibitor_I9; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; SSF52743; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Direct protein sequencing; Disulfide bond;
KW Hydrolase; Metal-binding; Protease; Serine protease; Signal; Zymogen.
FT SIGNAL 1..15
FT PROPEP 16..105
FT /evidence="ECO:0000269|Ref.2, ECO:0000269|Ref.3"
FT /id="PRO_0000027138"
FT CHAIN 106..384
FT /note="Proteinase K"
FT /id="PRO_0000027139"
FT DOMAIN 39..104
FT /note="Inhibitor I9"
FT /evidence="ECO:0000255"
FT DOMAIN 112..384
FT /note="Peptidase S8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 144
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 174
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT ACT_SITE 329
FT /note="Charge relay system"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT BINDING 280
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT BINDING 282
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT BINDING 305
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT BINDING 365
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT DISULFID 139..228
FT DISULFID 283..354
FT CONFLICT 184
FT /note="S -> SG (in Ref. 2; AA sequence and 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 312..316
FT /note="SILST -> DLS (in Ref. 2; AA sequence, 3; AA sequence
FT and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 374
FT /note="V -> F (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="Missing (in Ref. 2; AA sequence and 3; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:5ROS"
FT HELIX 113..118
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:2V8B"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:5ROS"
FT TURN 132..137
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 138..145
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 152..154
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 158..166
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 174..183
FT /evidence="ECO:0007829|PDB:2PWA"
FT TURN 185..187
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 194..199
FT /evidence="ECO:0007829|PDB:2PWA"
FT TURN 203..205
FT /evidence="ECO:0007829|PDB:6CLA"
FT HELIX 209..222
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 231..236
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 238..241
FT /evidence="ECO:0007829|PDB:4ZAR"
FT HELIX 244..255
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 258..263
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 266..270
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 271..273
FT /evidence="ECO:0007829|PDB:2PWA"
FT TURN 276..278
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 282..288
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 292..294
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 300..303
FT /evidence="ECO:0007829|PDB:6PKK"
FT STRAND 306..309
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 311..317
FT /evidence="ECO:0007829|PDB:2PWA"
FT TURN 318..320
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 321..325
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 328..344
FT /evidence="ECO:0007829|PDB:2PWA"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:2PWA"
FT HELIX 353..360
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 361..364
FT /evidence="ECO:0007829|PDB:2PWA"
FT STRAND 371..373
FT /evidence="ECO:0007829|PDB:6CLA"
SQ SEQUENCE 384 AA; 40300 MW; 00E1B111F0EFF5C4 CRC64;
MRLSVLLSLL PLALGAPAVE QRSEAAPLIE ARGEMVANKY IVKFKEGSAL SALDAAMEKI
SGKPDHVYKN VFSGFAATLD ENMVRVLRAH PDVEYIEQDA VVTINAAQTN APWGLARISS
TSPGTSTYYY DESAGQGSCV YVIDTGIEAS HPEFEGRAQM VKTYYYSSRD GNGHGTHCAG
TVGSRTYGVA KKTQLFGVKV LDDNGSGQYS TIIAGMDFVA SDKNNRNCPK GVVASLSLGG
GYSSSVNSAA ARLQSSGVMV AVAAGNNNAD ARNYSPASEP SVCTVGASDR YDRRSSFSNY
GSVLDIFGPG TSILSTWIGG STRSISGTSM ATPHVAGLAA YLMTLGKTTA ASACRYIADT
ANKGDLSNIP FGTVNLLAYN NYQA
