ID   PRTS_SERMA              Reviewed;        1045 AA.
AC   P09489;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Extracellular serine protease;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
OS   Serratia marcescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia.
OX   NCBI_TaxID=615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 28-35 AND
RP   407-408.
RC   STRAIN=NBRC 3046;
RX   PubMed=3011754; DOI=10.1128/jb.166.3.937-944.1986;
RA   Yanagida N., Uozumi T., Beppu T.;
RT   "Specific excretion of Serratia marcescens protease through the outer
RT   membrane of Escherichia coli.";
RL   J. Bacteriol. 166:937-944(1986).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE, AND PROTEOLYTIC PROCESSING.
RX   PubMed=1639760; DOI=10.1093/oxfordjournals.jbchem.a123809;
RA   Shikata S., Shimada K., Kataoka H., Hornouchi S., Beppu T.;
RT   "Detection of large COOH-terminal domains processed from the precursor of
RT   Serratia marcescens serine protease in the outer membrane of Escherichia
RT   coli.";
RL   J. Biochem. 111:627-632(1992).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; M13469; AAA26572.1; -; Genomic_DNA.
DR   PIR; A29840; A29840.
DR   AlphaFoldDB; P09489; -.
DR   SMR; P09489; -.
DR   STRING; 273526.SMDB11_1670; -.
DR   MEROPS; S08.094; -.
DR   TCDB; 1.B.12.5.1; the autotransporter-1 (at-1) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019867; C:outer membrane; IEA:InterPro.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd04848; Peptidases_S8_Autotransporter_serine_protease_like; 1.
DR   Gene3D; 2.40.128.130; -; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR005546; Autotransporte_beta.
DR   InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR   InterPro; IPR013425; Autotrns_rpt.
DR   InterPro; IPR006315; OM_autotransptr_brl.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR022398; Peptidase_S8_His-AS.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034061; Peptidases_S8_Autotransporter.
DR   Pfam; PF03797; Autotransporter; 1.
DR   Pfam; PF12951; PATR; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SMART; SM00869; Autotransporter; 1.
DR   SUPFAM; SSF103515; SSF103515; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   TIGRFAMs; TIGR01414; autotrans_barl; 1.
DR   TIGRFAMs; TIGR02601; autotrns_rpt; 1.
DR   PROSITE; PS51208; AUTOTRANSPORTER; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00137; SUBTILASE_HIS; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protease; Secreted; Serine protease;
KW   Signal; Zymogen.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:3011754"
FT   CHAIN           28..645
FT                   /note="Extracellular serine protease"
FT                   /id="PRO_0000027144"
FT   PROPEP          646..1045
FT                   /note="Translocator domain; removed in mature form"
FT                   /id="PRO_0000027145"
FT   DOMAIN          49..397
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DOMAIN          769..1045
FT                   /note="Autotransporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT   ACT_SITE        76
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        112
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        341
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   1045 AA;  112345 MW;  4924EA50E4FF179C CRC64;
     MILNKRLKLA YCVFLGCYGL SIHSSLAAYQ DPGRLGAPDS WKTAEFNRQW GLEAISAEFA
     YARGYTGKGI TIGVIDNAIL SHSEFSGKLT RLDNGSYNFS YDKQDNMSFG DHGTHVAGIA
     AAKRDGAGMH GVAFDADIIG TKLNDYGNRN GREELIQSAA RVINNSWGIA PDIRRDAKGD
     IIWLPNGRPD YVAFVKSEVI AEMMRSKSSV EWGSEQPVPT GGHSAMSTLL RAARHGKLIV
     FSAGNYNNYN IPEAQKSLPY AFPDVLNNYL IVTNLSDENQ LSVSSTSCGQ TASYCVSAPG
     SDIYSTVGRL ESNTGGAVNR EAYNKGELSL NPGYGNKSGT SMAAPHVTGV AAVLMQRFPY
     MSADQISAVI KTTATDLGVA GIDNLFGWGR VNLRDAINGP KMFITKEDIP QEYYVPGSYS
     EKQFVVNIPG LGNIVEPGTP VERRCTSSEC SFDSWSNDIS GHGGLTKTGA GTLALLGNNT
     YRGDTWVKQG VLAIDGSVAS NVYIENSGTL SGEGTVGAFR AARSGSVAPG NGIGTLHVLH
     DAIFDRGSQY NVEVADNGRS DKIAARRAFL NGGSVNVSLE RSQNLLSQNE AQSLLGNKYT
     ILTTTDGVTG RFENANPSYP FVKVALDYRG NDVGLGITRT DASFDSLAST ENEKAVARAV
     ETLNATEPVT ETAKRSVAIP AAEEANLLQS DGGEAQAVNE EASIVAGHPI YESFLGFTSA
     RELQQATRQL SGQIHADMAS AQINESRYLR DTATERLRQA EGRRTATDIK ADDNGAWAKL
     LGSWGHASGN DNATGYQTST YGVLLGLDSE LFGDGRLGMM TGYTRTSLDG GYQSDAHSDN
     YHLGLYGDKR FGALALRAGG TYTWHRIDTS RSVNYGAQSD REKAKYNART GQLFIESGYD
     WTSDAVNLEP FANLAYTHYR NEEINEQGGA AALRGDKQSQ SATASTLGLR ADTEWQTDSV
     AIALRGELGW QHQYGKLERK TQLMFKRTDA AFDVNSVPVS RDGAILKAGV DVSINKNAVL
     SLGYGGQLSS NHQDNSVNAG LTWRF