PRVB2_MERAP
ID PRVB2_MERAP Reviewed; 108 AA.
AC P86750;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 03-AUG-2022, entry version 24.
DE RecName: Full=Parvalbumin beta 2 {ECO:0000303|PubMed:20586483};
OS Merluccius australis polylepis (Southern hake) (Gadus australis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Zeiogadaria; Gadariae; Gadiformes; Gadoidei; Merlucciidae; Merluccius.
OX NCBI_TaxID=307685;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, MASS SPECTROMETRY, AND ACETYLATION AT ALA-1.
RC TISSUE=Muscle {ECO:0000269|PubMed:20586483};
RX PubMed=20586483; DOI=10.1021/pr100163e;
RA Carrera M., Canas B., Vazquez J., Gallardo J.M.;
RT "Extensive de novo sequencing of new parvalbumin isoforms using a novel
RT combination of bottom-up proteomics, accurate molecular mass measurement by
RT FTICR-MS, and selected MS/MS ion monitoring.";
RL J. Proteome Res. 9:4393-4406(2010).
CC -!- FUNCTION: In muscle, parvalbumin is thought to be involved in
CC relaxation after contraction. It binds two calcium ions (By
CC similarity). {ECO:0000250|UniProtKB:P02622,
CC ECO:0000250|UniProtKB:P02624}.
CC -!- MASS SPECTROMETRY: Mass=11354.806; Mass_error=0.0164;
CC Method=Electrospray; Evidence={ECO:0000269|PubMed:20586483};
CC -!- MISCELLANEOUS: Is regarded as an important allergen.
CC {ECO:0000250|UniProtKB:P02622, ECO:0000269|PubMed:20586483}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is:
CC 4.14, its MW is: 11.55 kDa. {ECO:0000269|PubMed:20586483}.
CC -!- SIMILARITY: Belongs to the parvalbumin family. {ECO:0000255}.
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DR AlphaFoldDB; P86750; -.
DR SMR; P86750; -.
DR iPTMnet; P86750; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR008080; Parvalbumin.
DR PANTHER; PTHR11653; PTHR11653; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Acetylation; Allergen; Calcium; Direct protein sequencing; Metal-binding;
KW Muscle protein; Repeat.
FT CHAIN 1..108
FT /note="Parvalbumin beta 2"
FT /id="PRO_0000399410"
FT DOMAIN 38..73
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 77..108
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 51
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 53
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 55
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621"
FT BINDING 59
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621"
FT BINDING 62
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 90
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 92
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 94
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 96
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P02621"
FT BINDING 101
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P02621,
FT ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 1
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 5
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 6
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 11
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 15
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 16
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 27
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 32
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 35
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 38
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 43
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 44
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 45
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 50
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 52
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 54
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 58
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 63
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 64
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 65
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 67
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 68
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 77
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 83
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 86
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 87
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 97
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 105
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 107
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
SQ SEQUENCE 108 AA; 11320 MW; DBA2AE6C2F5A01B6 CRC64;
AFSGILAEAD IAAALKACEA ADSFNYKAFF AKVGLSAKSA DDIKKAFFVI DQDKSGFIEE
DELKLFLQVF SAGARALTDA ETKAFLKAGD SDGDGAIGVD EFAVLVKA
