PRVB4_MERAA
ID PRVB4_MERAA Reviewed; 69 AA.
AC P86746;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=Parvalbumin beta 4 {ECO:0000303|PubMed:20586483};
DE Flags: Fragments;
OS Merluccius australis australis (Austral hake).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Zeiogadaria; Gadariae; Gadiformes; Gadoidei; Merlucciidae; Merluccius.
OX NCBI_TaxID=307686;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, AND ACETYLATION AT ALA-1.
RC TISSUE=Muscle {ECO:0000269|PubMed:20586483};
RX PubMed=20586483; DOI=10.1021/pr100163e;
RA Carrera M., Canas B., Vazquez J., Gallardo J.M.;
RT "Extensive de novo sequencing of new parvalbumin isoforms using a novel
RT combination of bottom-up proteomics, accurate molecular mass measurement by
RT FTICR-MS, and selected MS/MS ion monitoring.";
RL J. Proteome Res. 9:4393-4406(2010).
CC -!- FUNCTION: In muscle, parvalbumin is thought to be involved in
CC relaxation after contraction. It binds two calcium ions (By
CC similarity). {ECO:0000250|UniProtKB:P02622,
CC ECO:0000250|UniProtKB:P02624}.
CC -!- MISCELLANEOUS: Is regarded as an important allergen.
CC {ECO:0000250|UniProtKB:P02622, ECO:0000269|PubMed:20586483}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is:
CC 3.98, its MW is: 11.33 kDa. {ECO:0000269|PubMed:20586483}.
CC -!- SIMILARITY: Belongs to the parvalbumin family. {ECO:0000255}.
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DR AlphaFoldDB; P86746; -.
DR SMR; P86746; -.
DR iPTMnet; P86746; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR008080; Parvalbumin.
DR PANTHER; PTHR11653; PTHR11653; 1.
DR Pfam; PF13405; EF-hand_6; 1.
DR SMART; SM00054; EFh; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Allergen; Calcium; Direct protein sequencing; Metal-binding;
KW Muscle protein.
FT CHAIN 1..>69
FT /note="Parvalbumin beta 4"
FT /id="PRO_0000399408"
FT DOMAIN 24..59
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 37
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 41
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 43
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P02621"
FT BINDING 48
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 1
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 6
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 11
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 12
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 15
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 27
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 31
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 35
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 36
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 38
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 44
FT /note="I or L"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 49
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 50
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 51
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 53
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 54
FT /note="Q or K"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 63
FT /note="L or I"
FT /evidence="ECO:0000269|PubMed:20586483"
FT UNSURE 69
FT /note="K or Q"
FT /evidence="ECO:0000269|PubMed:20586483"
FT NON_CONS 31..32
FT /evidence="ECO:0000303|PubMed:20586483"
FT NON_TER 69
FT /evidence="ECO:0000303|PubMed:20586483"
SQ SEQUENCE 69 AA; 7426 MW; 76D66246D0837394 CRC64;
AFAGVLADAD IKAALAGCAA ADSFNYKTFF KFFAIIDQDH SGFIEEEELK LFLQTFSAGA
RALSDAETK
