PRVT_CHICK
ID PRVT_CHICK Reviewed; 109 AA.
AC P19753;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Parvalbumin, thymic;
DE AltName: Full=Avian thymic hormone;
DE Short=ATH;
DE AltName: Full=Thymus-specific antigen T1;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1897928; DOI=10.1016/0003-9861(91)90351-i;
RA Palmisano W.A., Henzl M.T.;
RT "Molecular cloning of the thymus-specific parvalbumin known as avian thymic
RT hormone: isolation of a full length cDNA and expression of the recombinant
RT protein in Escherichia coli.";
RL Arch. Biochem. Biophys. 285:211-220(1991).
RN [2]
RP PROTEIN SEQUENCE OF 2-109, AND ACETYLATION AT ALA-2.
RC TISSUE=Thymus;
RX PubMed=2126205; DOI=10.1016/0300-9084(90)90047-k;
RA Brewer J.M., Wunderlich J.K., Ragland W.L.;
RT "The amino acid sequence of avian thymic hormone, a parvalbumin.";
RL Biochimie 72:653-660(1990).
RN [3]
RP PRELIMINARY PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Thymus;
RX PubMed=2499324; DOI=10.1016/s0006-291x(89)80124-x;
RA Brewer J.M., Wunderlich J.K., Kim D.-H., Carr M.Y., Beach G.G.,
RA Ragland W.L.;
RT "Avian thymic hormone (ATH) is a parvalbumin.";
RL Biochem. Biophys. Res. Commun. 160:1155-1161(1989).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 41-103.
RX PubMed=2322274; DOI=10.1016/0006-291x(90)90663-8;
RA Palmisano W.A., Henzl M.T.;
RT "Partial nucleotide sequence of the parvalbumin from chicken thymus
RT designated 'avian thymic hormone'.";
RL Biochem. Biophys. Res. Commun. 167:1286-1293(1990).
RN [5]
RP STRUCTURE BY NMR OF 2-109, AND X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX PubMed=20156445; DOI=10.1016/j.jmb.2010.02.014;
RA Schuermann J.P., Tan A., Tanner J.J., Henzl M.T.;
RT "Structure of avian thymic hormone, a high-affinity avian beta-parvalbumin,
RT in the Ca2+-free and Ca2+-bound states.";
RL J. Mol. Biol. 397:991-1002(2010).
CC -!- FUNCTION: Appears to promote immune maturation in bone marrow cells in
CC culture. Binds two calcium ions.
CC -!- MISCELLANEOUS: This parvalbumin has an isoelectric point of 4.3.
CC -!- SIMILARITY: Belongs to the parvalbumin family. {ECO:0000305}.
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DR EMBL; M94894; AAA48605.1; -; mRNA.
DR EMBL; M34330; AAA48606.1; -; mRNA.
DR PIR; S13911; S13911.
DR RefSeq; NP_001007478.1; NM_001007477.3.
DR PDB; 2KQY; NMR; -; A=2-109.
DR PDB; 3FS7; X-ray; 1.95 A; A/B/C/D/E/F/G/H=1-109.
DR PDBsum; 2KQY; -.
DR PDBsum; 3FS7; -.
DR AlphaFoldDB; P19753; -.
DR BMRB; P19753; -.
DR SMR; P19753; -.
DR STRING; 9031.ENSGALP00000033676; -.
DR iPTMnet; P19753; -.
DR PaxDb; P19753; -.
DR Ensembl; ENSGALT00000093010; ENSGALP00000065536; ENSGALG00000053246.
DR GeneID; 396531; -.
DR KEGG; gga:396531; -.
DR CTD; 595049; -.
DR VEuPathDB; HostDB:geneid_396531; -.
DR eggNOG; KOG0027; Eukaryota.
DR GeneTree; ENSGT00940000165760; -.
DR HOGENOM; CLU_157356_0_0_1; -.
DR InParanoid; P19753; -.
DR OMA; SSCQAAD; -.
DR OrthoDB; 1524081at2759; -.
DR PhylomeDB; P19753; -.
DR EvolutionaryTrace; P19753; -.
DR PRO; PR:P19753; -.
DR Proteomes; UP000000539; Chromosome 14.
DR Bgee; ENSGALG00000053246; Expressed in muscle tissue and 1 other tissue.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR008080; Parvalbumin.
DR PANTHER; PTHR11653; PTHR11653; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 2.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Calcium; Direct protein sequencing;
KW Metal-binding; Reference proteome; Repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2126205"
FT CHAIN 2..109
FT /note="Parvalbumin, thymic"
FT /id="PRO_0000073626"
FT DOMAIN 39..74
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 78..109
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 52
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 54
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 56
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 63
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 91
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 93
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 95
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 97
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 102
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:2126205"
FT CONFLICT 96
FT /note="G -> D (in Ref. 4; AAA48606)"
FT /evidence="ECO:0000305"
FT HELIX 3..5
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 9..18
FT /evidence="ECO:0007829|PDB:3FS7"
FT STRAND 19..21
FT /evidence="ECO:0007829|PDB:2KQY"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:2KQY"
FT HELIX 27..34
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:2KQY"
FT HELIX 41..51
FT /evidence="ECO:0007829|PDB:3FS7"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:2KQY"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 61..65
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 80..90
FT /evidence="ECO:0007829|PDB:3FS7"
FT STRAND 95..98
FT /evidence="ECO:0007829|PDB:3FS7"
FT HELIX 100..107
FT /evidence="ECO:0007829|PDB:3FS7"
SQ SEQUENCE 109 AA; 11726 MW; 8A532C55499CB632 CRC64;
MAITDILSAK DIESALSSCQ AADSFNYKSF FSTVGLSSKT PDQIKKVFGI LDQDKSGFIE
EEELQLFLKN FSSSARVLTS AETKAFLAAG DTDGDGKIGV EEFQSLVKA
