PRX5_PENRW
ID PRX5_PENRW Reviewed; 544 AA.
AC B6H059;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=MFS-type transporter prx5 {ECO:0000303|PubMed:24239699};
DE AltName: Full=PR-toxin biosynthesis cluster protein 5 {ECO:0000303|PubMed:24239699};
GN Name=prx5 {ECO:0000303|PubMed:24239699};
GN ORFNames=Pc12g06260, PCH_Pc12g06260;
OS Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin
OS 54-1255) (Penicillium chrysogenum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium;
OC Penicillium chrysogenum species complex.
OX NCBI_TaxID=500485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255;
RX PubMed=18820685; DOI=10.1038/nbt.1498;
RA van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M.,
RA Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M.,
RA Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F.,
RA Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A., van der Klei I.J.,
RA van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R.,
RA Bovenberg R.A.L.;
RT "Genome sequencing and analysis of the filamentous fungus Penicillium
RT chrysogenum.";
RL Nat. Biotechnol. 26:1161-1168(2008).
RN [2]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=24239699; DOI=10.1016/j.fgb.2013.10.009;
RA Hidalgo P.I., Ullan R.V., Albillos S.M., Montero O., Fernandez-Bodega M.A.,
RA Garcia-Estrada C., Fernandez-Aguado M., Martin J.F.;
RT "Molecular characterization of the PR-toxin gene cluster in Penicillium
RT roqueforti and Penicillium chrysogenum: cross talk of secondary metabolite
RT pathways.";
RL Fungal Genet. Biol. 62:11-24(2014).
CC -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC the biosynthesis of PR-toxin, a bicyclic sesquiterpene belonging to the
CC eremophilane class and acting as a mycotoxin.
CC {ECO:0000269|PubMed:24239699}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression and the subsequent production of PR-toxin take
CC place under static culture conditions (oxygen limited), whereas no
CC expression of the PR-toxin genes occurs under the strongly aerated
CC conditions required for optimal penicillin production
CC (PubMed:24239699). There is a negative control of the transcription of
CC the PR-toxin genes by the penicillin biosynthesis gene product(s), or
CC by a regulatory peptide encoded by a small ORF inside the penicillin
CC gene cluster (PubMed:24239699). {ECO:0000269|PubMed:24239699}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AM920427; CAP80253.1; -; Genomic_DNA.
DR RefSeq; XP_002557469.1; XM_002557423.1.
DR AlphaFoldDB; B6H059; -.
DR EnsemblFungi; CAP80253; CAP80253; PCH_Pc12g06260.
DR GeneID; 8313012; -.
DR KEGG; pcs:Pc12g06260; -.
DR VEuPathDB; FungiDB:PCH_Pc12g06260; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_25_2_1; -.
DR OMA; FWPIPVV; -.
DR OrthoDB; 920726at2759; -.
DR BioCyc; PCHR:PC12G06260-MON; -.
DR Proteomes; UP000000724; Contig Pc00c12.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR010573; MFS_Str1/Tri12-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF06609; TRI12; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..544
FT /note="MFS-type transporter prx5"
FT /id="PRO_0000451234"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 290..310
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 505..525
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 544 AA; 58379 MW; 4D76B398D56CBC85 CRC64;
MAVDTEKDSV QAGSPMETPG SPVDETTEPV VTSKTWIVSM ILSCGYGLSF WPIPVVSAIG
TMISADMGDP TGYIWTLCKE TNPIIGMDYL DHLCFLDLVC FIGHIVVASA KSTNQVIAGL
VVSGFGGANC QMAAFALPEL LPNKWRHIGV VIADLTVYIA VIIAPVTARY GYELGTWAWN
FWGVAIFQGL SFFGLLFLYH PPKHPLGIPY KEAFKSLDYL GAFLFIGGAV PFLMGIVWAG
VYDSNDVHVV APLVVGAAVL VCFALWESFG KLKYPLTPTY VFASSWGRDF TAPVIALGVV
NMFYYSSSIL WPQMITVFYT NGGADWKYSV ILSLPQGFAI FFGAMLLTCF GSKLRHWHWQ
LTGSVFVMVV FGSLLGIVTP TNKGTMIAFI FLSQAGFGWA LYLSIAITQM GVEHKNLGVS
GGISGCIRFA AGAVATSIYQ TVYSNSLAKY TAIYVPSAAI SAGLPESKVT DLMAVVSQGA
AAMKSYSPAV VAAAEAALSQ AYCKAIFVVA MVSMAFGILG LAACLCCKDV DSKMTNKPTI
IRRD
