PS12A_ARATH
ID PS12A_ARATH Reviewed; 442 AA.
AC Q9FIB6;
DT 24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=26S proteasome non-ATPase regulatory subunit 12 homolog A;
DE AltName: Full=26S proteasome regulatory subunit RPN5a;
DE Short=AtRPN5a;
DE AltName: Full=26S proteasome regulatory subunit p55 homolog A;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2107;
DE AltName: Full=Protein MARIPOSA;
GN Name=RPN5A; Synonyms=EMB2107, MSA; OrderedLocusNames=At5g09900;
GN ORFNames=MYH9.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, IDENTIFICATION BY MASS SPECTROMETRY,
RP AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=14623884; DOI=10.1074/jbc.m311977200;
RA Yang P., Fu H., Walker J., Papa C.M., Smalle J., Ju Y.-M., Vierstra R.D.;
RT "Purification of the Arabidopsis 26 S proteasome: biochemical and molecular
RT analyses revealed the presence of multiple isoforms.";
RL J. Biol. Chem. 279:6401-6413(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT features of the regions of 1,081,958 bp covered by seventeen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:379-391(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP DISRUPTION PHENOTYPE, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=19252082; DOI=10.1105/tpc.108.064444;
RA Book A.J., Smalle J., Lee K.H., Yang P., Walker J.M., Casper S.,
RA Holmes J.H., Russo L.A., Buzzinotti Z.W., Jenik P.D., Vierstra R.D.;
RT "The RPN5 subunit of the 26s proteasome is essential for gametogenesis,
RT sporophyte development, and complex assembly in Arabidopsis.";
RL Plant Cell 21:460-478(2009).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME
RP COMPLEX, SUBUNIT, AND CLEAVAGE OF INITIATOR METHIONINE.
RX PubMed=20516081; DOI=10.1074/jbc.m110.136622;
RA Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.;
RT "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse
RT array of plant proteolytic complexes.";
RL J. Biol. Chem. 285:25554-25569(2010).
CC -!- FUNCTION: Acts as a regulatory subunit of the 26 proteasome which is
CC involved in the ATP-dependent degradation of ubiquitinated proteins.
CC Required for gametogenesis and sporophyte development. Acts redundantly
CC with RPN5B. {ECO:0000269|PubMed:19252082}.
CC -!- SUBUNIT: Component of the 19S regulatory particle (RP/PA700) lid
CC subcomplex of the 26S proteasome. The 26S proteasome is composed of a
CC core protease (CP), known as the 20S proteasome, capped at one or both
CC ends by the 19S regulatory particle (RP/PA700). The RP/PA700 complex is
CC composed of at least 17 different subunits in two subcomplexes, the
CC base and the lid, which form the portions proximal and distal to the
CC 20S proteolytic core, respectively. {ECO:0000269|PubMed:14623884,
CC ECO:0000269|PubMed:20516081}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19252082}. Nucleus
CC {ECO:0000269|PubMed:19252082}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FIB6-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Ubiquitous with highest expression in flowers.
CC {ECO:0000269|PubMed:14623884}.
CC -!- DISRUPTION PHENOTYPE: Displays a host of morphogenetic defects,
CC including abnormal embryogenesis, partially deetiolated development in
CC the dark, a severely dwarfed phenotype when grown in the light, and
CC infertility. {ECO:0000269|PubMed:19252082}.
CC -!- SIMILARITY: Belongs to the proteasome subunit p55 family.
CC {ECO:0000305}.
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DR EMBL; AY230832; AAP86659.1; -; mRNA.
DR EMBL; AB016893; BAB09411.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91463.1; -; Genomic_DNA.
DR EMBL; AY034908; AAK59415.1; -; mRNA.
DR EMBL; AY113906; AAM44954.1; -; mRNA.
DR RefSeq; NP_196552.1; NM_121027.4. [Q9FIB6-1]
DR AlphaFoldDB; Q9FIB6; -.
DR SMR; Q9FIB6; -.
DR BioGRID; 16128; 103.
DR IntAct; Q9FIB6; 2.
DR PaxDb; Q9FIB6; -.
DR PRIDE; Q9FIB6; -.
DR EnsemblPlants; AT5G09900.1; AT5G09900.1; AT5G09900. [Q9FIB6-1]
DR GeneID; 830850; -.
DR Gramene; AT5G09900.1; AT5G09900.1; AT5G09900. [Q9FIB6-1]
DR KEGG; ath:AT5G09900; -.
DR Araport; AT5G09900; -.
DR HOGENOM; CLU_033860_1_0_1; -.
DR PhylomeDB; Q9FIB6; -.
DR PRO; PR:Q9FIB6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FIB6; baseline and differential.
DR Genevisible; Q9FIB6; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0031595; C:nuclear proteasome complex; IBA:GO_Central.
DR GO; GO:0008541; C:proteasome regulatory particle, lid subcomplex; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000717; PCI_dom.
DR InterPro; IPR040134; PSMD12/CSN4.
DR InterPro; IPR040896; RPN5_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10855; PTHR10855; 1.
DR Pfam; PF01399; PCI; 1.
DR Pfam; PF18098; RPN5_C; 1.
DR SMART; SM00088; PINT; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50250; PCI; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Nucleus; Proteasome;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000305|PubMed:20516081"
FT CHAIN 2..442
FT /note="26S proteasome non-ATPase regulatory subunit 12
FT homolog A"
FT /id="PRO_0000423171"
FT DOMAIN 232..403
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT COILED 6..137
FT /evidence="ECO:0000255"
SQ SEQUENCE 442 AA; 50837 MW; 94C67CD1E5C82764 CRC64;
MGDSGKLEAT IDRLLNEEKQ MRLAENVAGT RKAATEILQL CFDAKDWKLL NEQILNLSKK
RGQLKQAVQS MVQQAMQYID QTPDIETRIE LIKTLNNVSA GKIYVEIERA RLTKKLAKIK
EEQGQIAEAA DLMQEVAVET FGAMAKTEKI AFILEQVRLC LDRQDFVRAQ ILSRKINPRV
FDADTKKDKK KPKEGDNMVE EAPADIPTLL ELKRIYYELM IRYYSHNNEY IEICRSYKAI
YDIPSVKETP EQWIPVLRKI CWFLVLAPHD PMQSSLLNAT LEDKNLSEIP DFKMLLKQVV
TMEVIQWTSL WNKYKDEFEK EKSMIGGSLG DKAGEDLKLR IIEHNILVVS KYYARITLKR
LAELLCLSME EAEKHLSEMV VSKALIAKID RPSGIVCFQI AKDSNEILNS WAGNLEKLLD
LVEKSCHQIH KETMVHKAAL RP
