ATG41_YEAST
ID ATG41_YEAST Reviewed; 136 AA.
AC Q12048; D6W3C0;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Autophagy-related protein 41 {ECO:0000303|PubMed:26565778};
DE AltName: Full=Interacting with cytoskeleton protein 2 {ECO:0000303|PubMed:26565778};
GN Name=ATG41 {ECO:0000303|PubMed:26565778};
GN Synonyms=ICY2 {ECO:0000303|PubMed:26565778};
GN OrderedLocusNames=YPL250C {ECO:0000312|SGD:S000006171};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [6]
RP DISRUPTION PHENOTYPE, FUNCTION, INDUCTION, SUBCELLULAR LOCATION,
RP INTERACTION WITH ATG9, AND DOMAIN.
RX PubMed=26565778; DOI=10.1080/15548627.2015.1107692;
RA Yao Z., Delorme-Axford E., Backues S.K., Klionsky D.J.;
RT "Atg41/Icy2 regulates autophagosome formation.";
RL Autophagy 11:2288-2299(2015).
CC -!- FUNCTION: Involved in both selective and non-selective autophagy
CC (PubMed:26565778). Does not appear to play a role in determining the
CC size of autophagosomes, but rather influences their formation rate
CC (PubMed:26565778). With ATG9, plays a role in the delivery of donor
CC membrane to expanding phagophore (PubMed:26565778).
CC {ECO:0000269|PubMed:26565778}.
CC -!- SUBUNIT: Interacts with ATG9 (PubMed:26565778).
CC {ECO:0000269|PubMed:26565778}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000305|PubMed:26565778}; Peripheral membrane protein
CC {ECO:0000305|PubMed:26565778}. Note=Shows a peri-mitochondrial
CC distribution similar to ATG9 (PubMed:26565778).
CC {ECO:0000269|PubMed:26565778}.
CC -!- INDUCTION: Expression is dramatically increased under nitrogen
CC starvation conditions and is regulated by the transcription factor GCN4
CC (PubMed:26565778). {ECO:0000269|PubMed:26565778}.
CC -!- DOMAIN: The C-terminal 10 residues are required for the association
CC with ATG9 (PubMed:26565778). {ECO:0000269|PubMed:26565778}.
CC -!- DISRUPTION PHENOTYPE: Reduces the autophagy flux induced by nitrogen
CC starvation as well as the flux of the cytoplasm-to-vacuole targeting
CC (cvt) pathway (PubMed:26565778). {ECO:0000269|PubMed:26565778}.
CC -!- MISCELLANEOUS: Present with 450 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; AY692697; AAT92716.1; -; Genomic_DNA.
DR EMBL; Z67751; CAA91594.1; -; Genomic_DNA.
DR EMBL; Z73606; CAA97972.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11186.1; -; Genomic_DNA.
DR PIR; S61014; S61014.
DR RefSeq; NP_015073.1; NM_001184064.1.
DR AlphaFoldDB; Q12048; -.
DR BioGRID; 35912; 190.
DR DIP; DIP-8888N; -.
DR STRING; 4932.YPL250C; -.
DR iPTMnet; Q12048; -.
DR PaxDb; Q12048; -.
DR EnsemblFungi; YPL250C_mRNA; YPL250C; YPL250C.
DR GeneID; 855825; -.
DR KEGG; sce:YPL250C; -.
DR SGD; S000006171; ATG41.
DR VEuPathDB; FungiDB:YPL250C; -.
DR HOGENOM; CLU_149528_0_0_1; -.
DR InParanoid; Q12048; -.
DR OMA; STEDDCM; -.
DR BioCyc; YEAST:G3O-34136-MON; -.
DR PRO; PR:Q12048; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12048; protein.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000045; P:autophagosome assembly; IMP:SGD.
DR GO; GO:0000422; P:autophagy of mitochondrion; IMP:SGD.
DR GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IMP:SGD.
DR GO; GO:0016236; P:macroautophagy; IMP:SGD.
PE 1: Evidence at protein level;
KW Autophagy; Membrane; Reference proteome.
FT CHAIN 1..136
FT /note="Autophagy-related protein 41"
FT /id="PRO_0000270559"
FT REGION 127..136
FT /note="ATG9-binding"
FT /evidence="ECO:0000269|PubMed:26565778"
SQ SEQUENCE 136 AA; 14986 MW; FFC7714A8F7332F1 CRC64;
MSSVESSPIS RYEDEVFPLS FSNVAFEPPM LSHSPDRSTY ADDFSQSYQQ ELLTFPLSYP
IVDESECTHT KDKTDSNIIT STEDDCMFDM EFNGNAASAV AAASKESNSA SGFAFASNDA
FANVAQQNYR LWLSSV
