ATG43_SCHPO
ID ATG43_SCHPO Reviewed; 244 AA.
AC O13709; Q9Y828;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 14-AUG-2001, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Mitophagy receptor atg43 {ECO:0000303|PubMed:33138913};
GN Name=atg43 {ECO:0000303|PubMed:33138913};
GN ORFNames=SPAC14C4.01c {ECO:0000312|PomBase:SPAC14C4.01c},
GN SPAC19D5.08c {ECO:0000312|PomBase:SPAC14C4.01c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, INTERACTION WITH ATG8; MIM1 AND MIM2, SUBCELLULAR LOCATION,
RP INDUCTION, DOMAIN, DISRUPTION PHENOTYPE, TOPOLOGY, AND MUTAGENESIS OF
RP 28-TYR--ILE-31.
RX PubMed=33138913; DOI=10.7554/elife.61245;
RA Fukuda T., Ebi Y., Saigusa T., Furukawa K., Yamashita S.I., Inoue K.,
RA Kobayashi D., Yoshida Y., Kanki T.;
RT "Atg43 tethers isolation membranes to mitochondria to promote starvation-
RT induced mitophagy in fission yeast.";
RL Elife 9:61245-61245(2020).
CC -!- FUNCTION: Mitophagy receptor that tethers atg8 to the mitochondrial
CC outer membrane to promote selective autophagy.
CC {ECO:0000269|PubMed:33138913}.
CC -!- SUBUNIT: Interacts (via N-terminal atg8 interacting motif) with atg8;
CC the interaction is direct (PubMed:33138913). Interacts with the
CC mitochondrial outer import machinery (MIM) complex subunits mim1 and
CC mim2 (PubMed:33138913). {ECO:0000269|PubMed:33138913}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:33138913}; Single-pass type IV membrane protein
CC {ECO:0000269|PubMed:33138913}.
CC -!- INDUCTION: Increases during nitrogen starvation (at protein level)
CC (PubMed:33138913). Repressed in nutrient-replete conditions in a TORC1-
CC dependent manner (at protein level) (PubMed:33138913).
CC {ECO:0000269|PubMed:33138913}.
CC -!- DOMAIN: The atg8 interacting motif (AIM) is required for the
CC interaction with atg8 (PubMed:33138913). The AIM motif is required for
CC atg43 function as mitophagy receptor (PubMed:33138913).
CC {ECO:0000269|PubMed:33138913}.
CC -!- DISRUPTION PHENOTYPE: Abnormal mitophagy during nitrogen starvation
CC (PubMed:33138913). Decreases vegetative cell population growth
CC (PubMed:33138913). {ECO:0000269|PubMed:33138913}.
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DR EMBL; CU329670; CAB50790.1; -; Genomic_DNA.
DR PIR; T37686; T37686.
DR RefSeq; NP_594906.2; NM_001020337.2.
DR AlphaFoldDB; O13709; -.
DR BioGRID; 278177; 8.
DR STRING; 4896.SPAC14C4.01c.1; -.
DR iPTMnet; O13709; -.
DR MaxQB; O13709; -.
DR PaxDb; O13709; -.
DR PRIDE; O13709; -.
DR EnsemblFungi; SPAC14C4.01c.1; SPAC14C4.01c.1:pep; SPAC14C4.01c.
DR GeneID; 2541681; -.
DR KEGG; spo:SPAC14C4.01c; -.
DR PomBase; SPAC14C4.01c; -.
DR VEuPathDB; FungiDB:SPAC14C4.01c; -.
DR HOGENOM; CLU_1138567_0_0_1; -.
DR InParanoid; O13709; -.
DR OMA; CWITVRD; -.
DR PRO; PR:O13709; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; EXP:PomBase.
DR GO; GO:0005741; C:mitochondrial outer membrane; EXP:PomBase.
DR GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR GO; GO:0140580; F:mitochondrion autophagosome adaptor activity; EXP:PomBase.
DR GO; GO:0000423; P:mitophagy; EXP:PomBase.
DR InterPro; IPR013898; Atg43.
DR PANTHER; PTHR38699; PTHR38699; 1.
DR Pfam; PF08589; ATG43; 1.
PE 1: Evidence at protein level;
KW Autophagy; Membrane; Mitochondrion; Mitochondrion outer membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..244
FT /note="Mitophagy receptor atg43"
FT /id="PRO_0000116680"
FT TOPO_DOM 1..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:33138913"
FT TRANSMEM 199..215
FT /note="Helical"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:33138913"
FT TOPO_DOM 216..244
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000305|PubMed:33138913"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 105..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..184
FT /note="Involved in MIM complex binding. Required for normal
FT vegetative cell population growth but is dispensable for
FT mitophagy"
FT /evidence="ECO:0000269|PubMed:33138913"
FT MOTIF 28..31
FT /note="atg8 interacting motif (AIM)"
FT /evidence="ECO:0000269|PubMed:33138913"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 28..31
FT /note="YELI->AELA: Abolishes atg8 binding. Abnormal
FT mitophagy during nitrogen starvation. Sensitive to nitrogen
FT starvation."
FT /evidence="ECO:0000269|PubMed:33138913"
SQ SEQUENCE 244 AA; 27039 MW; E1CECAE7185B86E1 CRC64;
MSSESKGIPI PRSDSNKTSD VSSWEEDYEL ISLGSSQDAL QRSGIRSAHG DSGYASSPLR
MEHLSSSTII INNQLKKIDV NESIPENSTL HNKYELGAVE SSTSSLSLLQ SKEEDDSSNW
ETEDSESAVE EAELPTIFEG KTVISPSSSG TDHSEAVEYT VPTAPPIPDL RFQQSYLQSI
QRANGSAFLV ALITLRDHVL YPFLSGGMWV FVRHIFQFLK LQEKGFHFGQ SLRRNLGLFS
TFKD
