ID   PSA3C_ARATH             Reviewed;         277 AA.
AC   Q9M3C6;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 119.
DE   RecName: Full=Photosystem I assembly factor PSA3, chloroplastic {ECO:0000305};
DE   AltName: Full=Protein PHOTOSYSTEM I ASSEMBLY 3 {ECO:0000303|PubMed:28522455};
DE   AltName: Full=Protein PIGMENT DEFECTIVE 329 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=PSA3 {ECO:0000303|PubMed:28522455}; Synonyms=PDE329 {ECO:0000305};
GN   OrderedLocusNames=At3g55250 {ECO:0000312|Araport:AT3G55250,
GN   ECO:0000312|EMBL:AAM20644.1};
GN   ORFNames=T26I12.130 {ECO:0000312|EMBL:CAB75759.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Lohse M.-A., Schneider A., Mueller-Roeber B.;
RT   "AtCHoR, a novel calcium homeostasis regulator in plants.";
RL   Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   FUNCTION, INTERACTION WITH PYG7, SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=28522455; DOI=10.1104/pp.17.00524;
RA   Shen J., Williams-Carrier R., Barkan A.;
RT   "PSA3, a protein on the stromal face of the thylakoid membrane, promotes
RT   photosystem I accumulation in cooperation with the assembly factor PYG7.";
RL   Plant Physiol. 174:1850-1862(2017).
CC   -!- FUNCTION: Nuclear genome-encoded factor required for the accumulation
CC       of photosystem I (PSI). Functions as PSI biogenesis factor. Cooperates
CC       with PYG7 to promote the stable assembly of PSI in the thylakoid
CC       membrane. May target primarily the PsaC subunit. Does not seem to be
CC       required for the expression of chloroplast genes encoding PSI subunits.
CC       {ECO:0000269|PubMed:28522455}.
CC   -!- SUBUNIT: Interacts with PYG7. {ECO:0000269|PubMed:28522455}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:28522455}. Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250|UniProtKB:B4FSG1}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:B4FSG1}. Note=Associates with the stromal face
CC       of the thylakoid membrane. {ECO:0000250|UniProtKB:B4FSG1}.
CC   -!- DISRUPTION PHENOTYPE: Seedling lethality when homozygous. Pale green
CC       and slow growing phenotype when grown on MS medium supplemented with 2%
CC       sucrose. {ECO:0000269|PubMed:28522455}.
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DR   EMBL; AY173126; AAO43936.1; -; mRNA.
DR   EMBL; AL132954; CAB75759.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE79357.1; -; Genomic_DNA.
DR   EMBL; AY099793; AAM20644.1; -; mRNA.
DR   EMBL; AY128909; AAM91309.1; -; mRNA.
DR   PIR; T47664; T47664.
DR   RefSeq; NP_191085.1; NM_115383.3.
DR   AlphaFoldDB; Q9M3C6; -.
DR   STRING; 3702.AT3G55250.1; -.
DR   MetOSite; Q9M3C6; -.
DR   PaxDb; Q9M3C6; -.
DR   PRIDE; Q9M3C6; -.
DR   ProteomicsDB; 226385; -.
DR   EnsemblPlants; AT3G55250.1; AT3G55250.1; AT3G55250.
DR   GeneID; 824691; -.
DR   Gramene; AT3G55250.1; AT3G55250.1; AT3G55250.
DR   KEGG; ath:AT3G55250; -.
DR   Araport; AT3G55250; -.
DR   TAIR; locus:2100691; AT3G55250.
DR   eggNOG; ENOG502QUQN; Eukaryota.
DR   HOGENOM; CLU_078970_0_0_1; -.
DR   InParanoid; Q9M3C6; -.
DR   OMA; CCFGKDP; -.
DR   OrthoDB; 1419822at2759; -.
DR   PhylomeDB; Q9M3C6; -.
DR   PRO; PR:Q9M3C6; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9M3C6; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR   GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IDA:TAIR.
DR   GO; GO:0005829; C:cytosol; HDA:TAIR.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0005634; C:nucleus; HDA:TAIR.
DR   GO; GO:0098572; C:stromal side of plastid thylakoid membrane; IDA:UniProtKB.
DR   GO; GO:0048564; P:photosystem I assembly; IMP:UniProtKB.
DR   InterPro; IPR037736; PSA3.
DR   PANTHER; PTHR36770; PTHR36770; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Membrane; Plastid; Reference proteome; Thylakoid;
KW   Transit peptide.
FT   TRANSIT         1..45
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..277
FT                   /note="Photosystem I assembly factor PSA3, chloroplastic"
FT                   /id="PRO_0000440979"
SQ   SEQUENCE   277 AA;  31245 MW;  7E45AB6F803AC84B CRC64;
     MVVVTHISTS FHQISPSFFH LRLRNPSTTS SSRPKLDGGF ALSIRAYIEK PNSFSTFANK
     VIGSLPVIGL LARIISDEGG VGRDLVDFAE FRKRVGNKCT PDDSRAFYEF QQRRGKAGEP
     LYVLLCCWVA AVGAGLLKSE EILEGVTRVS ISNDLEFEEQ NFIALMTEAR QRRAKLNIDA
     PTIPMELRVE KALEGIYACC FRRGLIEEED EKLLKVMLIA VFPSVEKSEI ERIIKEKATR
     VEEGGEEENV MAKRLPKEAI QMQMKDLEFL QQQNIES