PSA4L_DROME
ID PSA4L_DROME Reviewed; 251 AA.
AC Q9VA12; B3DN24; Q8IS89;
DT 08-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Proteasome subunit alpha type-4-like;
GN Name=Prosalpha3T; ORFNames=CG1736;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Testis;
RX PubMed=12421421; DOI=10.1046/j.1365-2583.2002.00374.x;
RA Ma J., Katz E., Belote J.M.;
RT "Expression of proteasome subunit isoforms during spermatogenesis in
RT Drosophila melanogaster.";
RL Insect Mol. Biol. 11:627-639(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The proteasome is a multicatalytic proteinase complex which
CC is characterized by its ability to cleave peptides with Arg, Phe, Tyr,
CC Leu, and Glu adjacent to the leaving group at neutral or slightly basic
CC pH. The proteasome has an ATP-dependent proteolytic activity.
CC -!- SUBUNIT: The 26S proteasome consists of a 20S proteasome core and two
CC 19S regulatory subunits. The 20S proteasome core is composed of 28
CC subunits that are arranged in four stacked rings, resulting in a
CC barrel-shaped structure. The two end rings are each formed by seven
CC alpha subunits, and the two central rings are each formed by seven beta
CC subunits. The catalytic chamber with the active sites is on the inside
CC of the barrel (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12421421}.
CC -!- TISSUE SPECIFICITY: Testis, prominent after meiosis II. After meiosis,
CC predominantly localized to the haploid spermatid nuclei of the 64-cell
CC cysts, remaining during the elongation and condensation of the
CC spermatid nuclei. In mature, motile sperm, expression is seen
CC exclusively in the sperm head. {ECO:0000269|PubMed:12421421}.
CC -!- SIMILARITY: Belongs to the peptidase T1A family. {ECO:0000255|PROSITE-
CC ProRule:PRU00808}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACD81826.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF57116.1; -; Genomic_DNA.
DR EMBL; AY147240; AAN63094.1; -; Genomic_DNA.
DR EMBL; BT032812; ACD81826.1; ALT_INIT; mRNA.
DR RefSeq; NP_651843.1; NM_143586.3.
DR AlphaFoldDB; Q9VA12; -.
DR SMR; Q9VA12; -.
DR BioGRID; 68527; 1.
DR STRING; 7227.FBpp0085100; -.
DR PaxDb; Q9VA12; -.
DR PRIDE; Q9VA12; -.
DR DNASU; 43679; -.
DR EnsemblMetazoa; FBtr0085738; FBpp0085100; FBgn0261395.
DR GeneID; 43679; -.
DR KEGG; dme:Dmel_CG1736; -.
DR UCSC; CG1736-RA; d. melanogaster.
DR CTD; 43679; -.
DR FlyBase; FBgn0261395; Prosalpha3T.
DR VEuPathDB; VectorBase:FBgn0261395; -.
DR eggNOG; KOG0178; Eukaryota.
DR GeneTree; ENSGT00550000074827; -.
DR HOGENOM; CLU_035750_4_3_1; -.
DR InParanoid; Q9VA12; -.
DR OMA; FLYMGWD; -.
DR OrthoDB; 1222564at2759; -.
DR PhylomeDB; Q9VA12; -.
DR BioGRID-ORCS; 43679; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43679; -.
DR PRO; PR:Q9VA12; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0261395; Expressed in testis and 8 other tissues.
DR Genevisible; Q9VA12; DM.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005839; C:proteasome core complex; IBA:GO_Central.
DR GO; GO:0019773; C:proteasome core complex, alpha-subunit complex; ISS:UniProtKB.
DR GO; GO:0010498; P:proteasomal protein catabolic process; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:FlyBase.
DR Gene3D; 3.60.20.10; -; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR023332; Proteasome_alpha-type.
DR InterPro; IPR000426; Proteasome_asu_N.
DR InterPro; IPR001353; Proteasome_sua/b.
DR InterPro; IPR034647; Proteasome_subunit_alpha4.
DR PANTHER; PTHR11599:SF13; PTHR11599:SF13; 1.
DR Pfam; PF00227; Proteasome; 1.
DR Pfam; PF10584; Proteasome_A_N; 1.
DR SMART; SM00948; Proteasome_A_N; 1.
DR SUPFAM; SSF56235; SSF56235; 1.
DR PROSITE; PS00388; PROTEASOME_ALPHA_1; 1.
DR PROSITE; PS51475; PROTEASOME_ALPHA_2; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Proteasome; Reference proteome.
FT CHAIN 1..251
FT /note="Proteasome subunit alpha type-4-like"
FT /id="PRO_0000124110"
FT CONFLICT 74..75
FT /note="CA -> WP (in Ref. 1; AAN63094)"
FT /evidence="ECO:0000305"
FT CONFLICT 133
FT /note="F -> L (in Ref. 1; AAN63094)"
FT /evidence="ECO:0000305"
FT CONFLICT 141..142
FT /note="RF -> AS (in Ref. 1; AAN63094)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 28169 MW; B1AFF83978DB14D3 CRC64;
MARFFDSRTT IFSPEGRLYQ VEYAMEAASQ SGTCVGLLAK NGVLLATERS VDKLMDTSIP
VPRISWLNEN IACCATGNTA DGNVLVNQLR MIAQQYQFNF GEMIPCEQLV TNLCDIKQAY
TQYGGKRPFG VSFLYMGWDC RFGFQLYQSD PSGNYSGWKA TCIGRKSGAA MEMLQKELFS
KGYVSPSVEE AKDVAIKVMG MTLGRDSLTP EKLEIAFVQR YGNTTVFHIL EKNEIHRLIE
RNNNLKRRVG S
