ID   PSA5_ENCCU              Reviewed;         232 AA.
AC   Q8SRU3;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Probable proteasome subunit alpha type-5;
DE   AltName: Full=26S proteasome alpha-type subunit PUP2;
DE   AltName: Full=Multicatalytic endopeptidase complex subunit PUP2;
GN   Name=PUP2; OrderedLocusNames=ECU05_1400;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: The proteasome degrades poly-ubiquitinated proteins in the
CC       cytoplasm and in the nucleus. It is essential for the regulated
CC       turnover of proteins and for the removal of misfolded proteins. The
CC       proteasome is a multicatalytic proteinase complex that is characterized
CC       by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu
CC       adjacent to the leaving group at neutral or slightly basic pH. It has
CC       an ATP-dependent proteolytic activity (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: The 26S proteasome consists of a 20S proteasome core and two
CC       19S regulatory subunits. The 20S proteasome core is composed of 28
CC       subunits that are arranged in four stacked rings, resulting in a
CC       barrel-shaped structure. The two end rings are each formed by seven
CC       alpha subunits, and the two central rings are each formed by seven beta
CC       subunits. The catalytic chamber with the active sites is on the inside
CC       of the barrel (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: Belongs to the peptidase T1A family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00808}.
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DR   EMBL; AL590445; CAD26660.1; -; Genomic_DNA.
DR   RefSeq; NP_597483.1; NM_001041349.1.
DR   AlphaFoldDB; Q8SRU3; -.
DR   SMR; Q8SRU3; -.
DR   STRING; 284813.Q8SRU3; -.
DR   GeneID; 859150; -.
DR   KEGG; ecu:ECU05_1400; -.
DR   VEuPathDB; MicrosporidiaDB:ECU05_1400; -.
DR   HOGENOM; CLU_035750_4_2_1; -.
DR   InParanoid; Q8SRU3; -.
DR   OMA; FQVEYAR; -.
DR   OrthoDB; 1222564at2759; -.
DR   Proteomes; UP000000819; Chromosome V.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019773; C:proteasome core complex, alpha-subunit complex; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR   Gene3D; 3.60.20.10; -; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR023332; Proteasome_alpha-type.
DR   InterPro; IPR000426; Proteasome_asu_N.
DR   InterPro; IPR001353; Proteasome_sua/b.
DR   Pfam; PF00227; Proteasome; 1.
DR   Pfam; PF10584; Proteasome_A_N; 1.
DR   SMART; SM00948; Proteasome_A_N; 1.
DR   SUPFAM; SSF56235; SSF56235; 1.
DR   PROSITE; PS00388; PROTEASOME_ALPHA_1; 1.
DR   PROSITE; PS51475; PROTEASOME_ALPHA_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nucleus; Proteasome; Reference proteome.
FT   CHAIN           1..232
FT                   /note="Probable proteasome subunit alpha type-5"
FT                   /id="PRO_0000382756"
SQ   SEQUENCE   232 AA;  26291 MW;  9F8E7CC6EDBECF13 CRC64;
     MEDFKQSVNT YSSEGRIHQI EYAMKAMNLG TTTIGVRTKE FVILCSEKKI LSTLQNPKSV
     VKHYKIYDHI VLGFSGISGD TKTIVKRSRD FCISHTHMYG ENISVERLLK YLSSLSLRFG
     EEDEAKMIFR RPFGVSLIIA GFDTEPRLYS LDPSGSYISY KAKAIGSGHE VVEGILENEY
     EEYSGLDQSL RKVLCTLSKV MKDKISKDNV EVVVVTQEES KFLTPEEVSV YL