ATG4C_XENLA
ID ATG4C_XENLA Reviewed; 450 AA.
AC Q5XH30;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Cysteine protease ATG4C {ECO:0000305};
DE EC=3.4.22.- {ECO:0000250|UniProtKB:Q96DT6};
DE AltName: Full=Autophagy-related protein 4 homolog C {ECO:0000250|UniProtKB:Q96DT6};
GN Name=atg4c {ECO:0000250|UniProtKB:Q96DT6};
GN Synonyms=apg4c {ECO:0000250|UniProtKB:Q96DT6};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Cysteine protease that plays a key role in autophagy by
CC mediating both proteolytic activation and delipidation of ATG8 family
CC proteins. The protease activity is required for proteolytic activation
CC of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8
CC proteins to reveal a C-terminal glycine (By similarity). Exposure of
CC the glycine at the C-terminus is essential for ATG8 proteins
CC conjugation to phosphatidylethanolamine (PE) and insertion to
CC membranes, which is necessary for autophagy (By similarity). In
CC addition to the protease activity, also mediates delipidation of ATG8
CC family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8
CC proteins during macroautophagy (By similarity).
CC {ECO:0000250|UniProtKB:Q96DT6, ECO:0000250|UniProtKB:Q9Y4P1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[protein]-C-terminal L-amino acid-glycyl-
CC phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-
CC glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine;
CC Xref=Rhea:RHEA:67548, Rhea:RHEA-COMP:17323, Rhea:RHEA-COMP:17324,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:64612, ChEBI:CHEBI:172940,
CC ChEBI:CHEBI:172941; Evidence={ECO:0000250|UniProtKB:Q96DT6};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:67549;
CC Evidence={ECO:0000250|UniProtKB:Q96DT6};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q8BGE6}.
CC -!- SIMILARITY: Belongs to the peptidase C54 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC084245; AAH84245.1; -; mRNA.
DR RefSeq; NP_001088249.1; NM_001094780.1.
DR AlphaFoldDB; Q5XH30; -.
DR SMR; Q5XH30; -.
DR MEROPS; C54.004; -.
DR DNASU; 495080; -.
DR GeneID; 495080; -.
DR KEGG; xla:495080; -.
DR CTD; 495080; -.
DR Xenbase; XB-GENE-941092; atg4c.S.
DR OMA; EAVFIMK; -.
DR OrthoDB; 431748at2759; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 495080; Expressed in egg cell and 19 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0008234; F:cysteine-type peptidase activity; ISS:UniProtKB.
DR GO; GO:0006914; P:autophagy; ISS:UniProtKB.
DR GO; GO:0051697; P:protein delipidation; ISS:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR032915; ATG4C.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR005078; Peptidase_C54.
DR PANTHER; PTHR22624; PTHR22624; 1.
DR PANTHER; PTHR22624:SF38; PTHR22624:SF38; 1.
DR Pfam; PF03416; Peptidase_C54; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Cytoplasm; Hydrolase; Protease; Protein transport;
KW Reference proteome; Thiol protease; Transport; Ubl conjugation pathway.
FT CHAIN 1..450
FT /note="Cysteine protease ATG4C"
FT /id="PRO_0000215851"
FT ACT_SITE 112
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q9Y4P1"
FT ACT_SITE 336
FT /evidence="ECO:0000250|UniProtKB:Q9Y4P1"
FT ACT_SITE 338
FT /evidence="ECO:0000250|UniProtKB:Q9Y4P1"
SQ SEQUENCE 450 AA; 51768 MW; 336763FCA05549F8 CRC64;
MEASGTDDVE KLKSKFLSAW HNMKYSWVLK TKTYFKRNSP VFLLGKCYHF KYEDSGVTAD
DCSNSGSDSK EDLSGNVDEF RKDFISRIWL TYRKEFPQIE SSSWTTDCGW GCTLRTGQML
LAQGLLVHFL GRDWTWTEAL DIFCSESDFW TANTARKLDP SLEKSSPENE EYVSLGKQPL
QNSEKKRYSE DLHRKIISWF ADYPLAYFGL HQLVKLGKNS GKVAGDWYGP AVVSHLLRKA
IEESSDPELQ GITIYVAQDC TIYNADVYDL QCNKGNEKAV VILVPVRLGG ERTNMEYFEY
VKGILSLEFC IGIIGGKPKQ SYYFVGFQDD SLIYMDPHYC QSFVDVSIKN FPLESFHCPS
PKKMSFKKMD PSCTVGFYCR NAREFEKAAE ELTKVLKSST KQNYPLFTFV NGHAQDFDFV
CTPVYDQNDL FTEDEKKRLK RFSTEEFVLL