PSAC_PEA
ID PSAC_PEA Reviewed; 81 AA.
AC P10793;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Photosystem I iron-sulfur center {ECO:0000255|HAMAP-Rule:MF_01303};
DE EC=1.97.1.12 {ECO:0000255|HAMAP-Rule:MF_01303};
DE AltName: Full=9 kDa polypeptide {ECO:0000255|HAMAP-Rule:MF_01303};
DE AltName: Full=PSI-C {ECO:0000255|HAMAP-Rule:MF_01303};
DE AltName: Full=Photosystem I subunit VII {ECO:0000255|HAMAP-Rule:MF_01303};
DE AltName: Full=PsaC {ECO:0000255|HAMAP-Rule:MF_01303};
GN Name=psaC {ECO:0000255|HAMAP-Rule:MF_01303}; Synonyms=frxA;
OS Pisum sativum (Garden pea).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX AGRICOLA=IND92000055; DOI=10.1007/BF00027388;
RA Dunn P.P.J., Gray J.C.;
RT "Localization and nucleotide sequence of the gene for the 8 kDa subunit of
RT photosystem I in pea and wheat chloroplast DNA.";
RL Plant Mol. Biol. 11:311-319(1988).
RN [2]
RP PROTEIN SEQUENCE OF 2-49.
RX PubMed=3277857; DOI=10.1016/0014-5793(88)80607-0;
RA Dunn P.P.J., Packman L.C., Pappin D., Gray J.C.;
RT "N-terminal amino acid sequence analysis of the subunits of pea photosystem
RT I.";
RL FEBS Lett. 228:157-161(1988).
CC -!- FUNCTION: Apoprotein for the two 4Fe-4S centers FA and FB of
CC photosystem I (PSI); essential for photochemical activity. FB is the
CC terminal electron acceptor of PSI, donating electrons to ferredoxin.
CC The C-terminus interacts with PsaA/B/D and helps assemble the protein
CC into the PSI complex. Required for binding of PsaD and PsaE to PSI. PSI
CC is a plastocyanin-ferredoxin oxidoreductase, converting photonic
CC excitation into a charge separation, which transfers an electron from
CC the donor P700 chlorophyll pair to the spectroscopically characterized
CC acceptors A0, A1, FX, FA and FB in turn. {ECO:0000255|HAMAP-
CC Rule:MF_01303}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hnu + oxidized [2Fe-2S]-[ferredoxin] + reduced [plastocyanin]
CC = oxidized [plastocyanin] + reduced [2Fe-2S]-[ferredoxin];
CC Xref=Rhea:RHEA:30407, Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001,
CC Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:29036,
CC ChEBI:CHEBI:30212, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC ChEBI:CHEBI:49552; EC=1.97.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01303};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01303};
CC Note=Binds 2 [4Fe-4S] clusters. Cluster 2 is most probably the
CC spectroscopically characterized electron acceptor FA and cluster 1 is
CC most probably FB. {ECO:0000255|HAMAP-Rule:MF_01303};
CC -!- SUBUNIT: The eukaryotic PSI reaction center is composed of at least 11
CC subunits. {ECO:0000255|HAMAP-Rule:MF_01303}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_01303}; Peripheral membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01303}; Stromal side {ECO:0000255|HAMAP-
CC Rule:MF_01303}.
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DR EMBL; X13157; CAA31554.1; -; Genomic_DNA.
DR PIR; S04033; FEPM1S.
DR RefSeq; YP_003587533.1; NC_014057.1.
DR PDB; 2O01; X-ray; 3.40 A; C=2-81.
DR PDB; 2WSC; X-ray; 3.30 A; C=1-81.
DR PDB; 2WSE; X-ray; 3.49 A; C=1-81.
DR PDB; 2WSF; X-ray; 3.48 A; C=1-81.
DR PDB; 3LW5; X-ray; 3.30 A; C=1-81.
DR PDB; 4RKU; X-ray; 3.00 A; C=2-81.
DR PDB; 4XK8; X-ray; 2.80 A; C/c=2-81.
DR PDB; 4Y28; X-ray; 2.80 A; C=1-81.
DR PDB; 5L8R; X-ray; 2.60 A; C=1-81.
DR PDB; 6LY5; EM; 2.38 A; c=2-81.
DR PDB; 6YAC; EM; 2.50 A; C=2-81.
DR PDB; 6YEZ; EM; 2.70 A; C=2-81.
DR PDB; 6ZOO; EM; 2.74 A; C=2-81.
DR PDB; 6ZXS; X-ray; 3.00 A; C=2-81.
DR PDB; 7DKZ; X-ray; 2.39 A; C=1-81.
DR PDBsum; 2O01; -.
DR PDBsum; 2WSC; -.
DR PDBsum; 2WSE; -.
DR PDBsum; 2WSF; -.
DR PDBsum; 3LW5; -.
DR PDBsum; 4RKU; -.
DR PDBsum; 4XK8; -.
DR PDBsum; 4Y28; -.
DR PDBsum; 5L8R; -.
DR PDBsum; 6LY5; -.
DR PDBsum; 6YAC; -.
DR PDBsum; 6YEZ; -.
DR PDBsum; 6ZOO; -.
DR PDBsum; 6ZXS; -.
DR PDBsum; 7DKZ; -.
DR AlphaFoldDB; P10793; -.
DR SMR; P10793; -.
DR DIP; DIP-60283N; -.
DR IntAct; P10793; 3.
DR GeneID; 9073063; -.
DR EvolutionaryTrace; P10793; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009522; C:photosystem I; IEA:UniProtKB-KW.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0009773; P:photosynthetic electron transport in photosystem I; IEA:InterPro.
DR HAMAP; MF_01303; PSI_PsaC; 1.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR017491; PSI_PsaC.
DR TIGRFAMs; TIGR03048; PS_I_psaC; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 2.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; 4Fe-4S; Chloroplast; Direct protein sequencing;
KW Electron transport; Iron; Iron-sulfur; Membrane; Metal-binding;
KW Oxidoreductase; Photosynthesis; Photosystem I; Plastid; Repeat; Thylakoid;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:3277857"
FT CHAIN 2..81
FT /note="Photosystem I iron-sulfur center"
FT /id="PRO_0000061996"
FT DOMAIN 2..31
FT /note="4Fe-4S ferredoxin-type 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT DOMAIN 39..68
FT /note="4Fe-4S ferredoxin-type 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 11
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 14
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 17
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 21
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 48
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 51
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 54
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT BINDING 58
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_label="1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01303"
FT STRAND 4..7
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 16..20
FT /evidence="ECO:0007829|PDB:6LY5"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 27..30
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:6LY5"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 38..41
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 45..47
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 53..57
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 60..62
FT /evidence="ECO:0007829|PDB:6LY5"
FT STRAND 64..68
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 74..77
FT /evidence="ECO:0007829|PDB:6LY5"
SQ SEQUENCE 81 AA; 8980 MW; 680556D8CB5603BF CRC64;
MSHSVKIYDT CIGCTQCVRA CPTDVLEMIP WGGCKAKQIA SAPRTEDCVG CKRCESACPT
DFLSVRVYLW HETTRSMGLA Y
