PSAD_SPIOL
ID PSAD_SPIOL Reviewed; 212 AA.
AC P12353; Q43642; Q9S8Z2; Q9S8Z3;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2001, sequence version 2.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Photosystem I reaction center subunit II, chloroplastic;
DE AltName: Full=Photosystem I 20 kDa subunit;
DE Short=PSI-D;
DE Flags: Precursor;
GN Name=psaD;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Seedling;
RX PubMed=3066511; DOI=10.1007/bf00521277;
RA Muench S., Ljungberg U., Steppuhn J., Schneiderbauer A., Nechushtai R.,
RA Beyreuther K., Herrmann R.G.;
RT "Nucleotide sequences of cDNAs encoding the entire precursor polypeptides
RT for subunits II and III of the photosystem I reaction center from
RT spinach.";
RL Curr. Genet. 14:511-518(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Leaf;
RX PubMed=3288500; DOI=10.1016/0014-5793(88)80752-x;
RA Lagoutte B.;
RT "Cloning and sequencing of spinach cDNA clones encoding the 20 kDa PS I
RT polypeptide.";
RL FEBS Lett. 232:275-280(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=cv. Monatol; TISSUE=Seedling;
RX PubMed=7920722; DOI=10.1046/j.1365-313x.1994.06030359.x;
RA Flieger K., Wicke A., Herrmann R.G., Oelmueller R.;
RT "Promoter and leader sequences of the spinach PsaD and PsaF genes direct an
RT opposite light response in tobacco cotyledons: PsaD sequences downstream of
RT the ATG codon are required for a positive light response.";
RL Plant J. 6:359-368(1994).
RN [4]
RP SEQUENCE REVISION.
RA Oelmueller R.;
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 51-63.
RX PubMed=3049567; DOI=10.1093/oxfordjournals.jbchem.a122394;
RA Oh-oka H., Takahashi Y., Kuriyama K., Saeki K., Matsubara H.;
RT "The protein responsible for center A/B in spinach photosystem I: isolation
RT with iron-sulfur cluster(s) and complete sequence analysis.";
RL J. Biochem. 103:962-968(1988).
RN [6]
RP PARTIAL PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RX PubMed=1374333; DOI=10.1111/j.1432-1033.1992.tb16888.x;
RA Lagoutte B., Vallon O.;
RT "Purification and membrane topology of PSI-D and PSI-E, two subunits of the
RT photosystem I reaction center.";
RL Eur. J. Biochem. 205:1175-1185(1992).
CC -!- FUNCTION: PsaD can form complexes with ferredoxin and ferredoxin-
CC oxidoreductase in photosystem I (PS I) reaction center. PSAD may encode
CC the ferredoxin-docking protein.
CC -!- INTERACTION:
CC P12353; P00221: PETF; NbExp=2; IntAct=EBI-864919, EBI-864933;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:1374333}; Peripheral membrane protein
CC {ECO:0000269|PubMed:1374333}; Stromal side
CC {ECO:0000269|PubMed:1374333}.
CC -!- INDUCTION: By light. {ECO:0000269|PubMed:7920722}.
CC -!- SIMILARITY: Belongs to the PsaD family. {ECO:0000305}.
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DR EMBL; X14017; CAA32182.1; -; mRNA.
DR EMBL; Y00759; CAA68728.1; -; mRNA.
DR EMBL; X77674; CAA54744.1; -; Genomic_DNA.
DR PIR; S03016; A1SP2.
DR PDB; 2O01; X-ray; 3.40 A; D=75-212.
DR PDB; 2WSC; X-ray; 3.30 A; D=1-212.
DR PDB; 2WSE; X-ray; 3.49 A; D=1-212.
DR PDB; 2WSF; X-ray; 3.48 A; D=1-212.
DR PDBsum; 2O01; -.
DR PDBsum; 2WSC; -.
DR PDBsum; 2WSE; -.
DR PDBsum; 2WSF; -.
DR AlphaFoldDB; P12353; -.
DR SMR; P12353; -.
DR IntAct; P12353; 1.
DR PRIDE; P12353; -.
DR OrthoDB; 1477247at2759; -.
DR EvolutionaryTrace; P12353; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009538; C:photosystem I reaction center; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR InterPro; IPR003685; PsaD.
DR InterPro; IPR036579; PsaD_sf.
DR PANTHER; PTHR31982; PTHR31982; 1.
DR Pfam; PF02531; PsaD; 1.
DR SUPFAM; SSF64234; SSF64234; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Membrane;
KW Photosynthesis; Photosystem I; Plastid; Thylakoid; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:3049567"
FT CHAIN 51..212
FT /note="Photosystem I reaction center subunit II,
FT chloroplastic"
FT /id="PRO_0000029377"
FT REGION 53..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 145..153
FT /note="Ferredoxin and ferredoxin-oxidoreductase binding"
FT /evidence="ECO:0000255"
FT CONFLICT 4..6
FT /note="ATQ -> GTP (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT CONFLICT 12
FT /note="P -> R (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="D -> E (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT CONFLICT 35..36
FT /note="VT -> LS (in Ref. 1; CAA32182)"
FT /evidence="ECO:0000305"
FT CONFLICT 45..47
FT /note="HHS -> LHT (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="A -> R (in Ref. 2; CAA68728)"
FT /evidence="ECO:0000305"
FT CONFLICT 60..61
FT /note="AA -> TP (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 68..70
FT /note="PKG -> TKA (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT CONFLICT 91
FT /note="Missing (in Ref. 3; CAA54744)"
FT /evidence="ECO:0000305"
FT STRAND 89..91
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 97..99
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 103..105
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:2O01"
FT TURN 116..119
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 130..132
FT /evidence="ECO:0007829|PDB:2WSC"
FT TURN 136..139
FT /evidence="ECO:0007829|PDB:2WSC"
FT TURN 141..147
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:2O01"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:2WSC"
FT TURN 168..170
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 171..173
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 175..177
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 189..191
FT /evidence="ECO:0007829|PDB:2WSC"
FT TURN 194..197
FT /evidence="ECO:0007829|PDB:2WSC"
FT STRAND 206..208
FT /evidence="ECO:0007829|PDB:2O01"
SQ SEQUENCE 212 AA; 23103 MW; BC5FF64D97A6570E CRC64;
MAMATQATLF SPSSLSSAKP IDTRLTTSFK QPSAVTFASK PASRHHSIRA AAAAEGKAAA
ATETKEAPKG FTPPELDPNT PSPIFAGSTG GLLRKAQVEE FYVITWESPK EQIFEMPTGG
AAIMREGPNL LKLARKEQCL ALGTRLRSKY KIKYQFYRVF PSGEVQYLHP KDGVYPEKVN
PGRQGVGLNM RSIGKNVSPI EVKFTGKQPY DL
