ATG5_AJECN
ID ATG5_AJECN Reviewed; 333 AA.
AC A6RE26;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Autophagy protein 5;
GN Name=ATG5; ORFNames=HCAG_07891;
OS Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus)
OS (Histoplasma capsulatum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma;
OC unclassified Histoplasma.
OX NCBI_TaxID=2059318;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NAm1 / WU24;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
CC -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC autophagic vesicle formation. Autophagy is essential for maintenance of
CC amino acid levels and protein synthesis under nitrogen starvation.
CC Required for selective autophagic degradation of the nucleus
CC (nucleophagy). Also required for mitophagy, which eliminates defective
CC or superfluous mitochondria in order to fulfill cellular energy
CC requirements and prevent excess ROS production. Conjugation with ATG12,
CC through a ubiquitin-like conjugating system involving ATG7 as an E1-
CC like activating enzyme and ATG10 as an E2-like conjugating enzyme, is
CC essential for its function. The ATG12-ATG5 conjugate acts as an E3-like
CC enzyme which is required for lipidation of ATG8 and ATG8 association to
CC the vesicle membranes (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- PTM: Conjugated to ATG12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR EMBL; CH476663; EDN11431.1; -; Genomic_DNA.
DR RefSeq; XP_001537575.1; XM_001537525.1.
DR AlphaFoldDB; A6RE26; -.
DR SMR; A6RE26; -.
DR STRING; 339724.A6RE26; -.
DR EnsemblFungi; EDN11431; EDN11431; HCAG_07891.
DR GeneID; 5443832; -.
DR KEGG; aje:HCAG_07891; -.
DR VEuPathDB; FungiDB:HCAG_07891; -.
DR HOGENOM; CLU_051894_2_0_1; -.
DR OMA; KWHYPLG; -.
DR OrthoDB; 457861at2759; -.
DR Proteomes; UP000009297; Unassembled WGS sequence.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..333
FT /note="Autophagy protein 5"
FT /id="PRO_0000317848"
FT REGION 107..133
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 256..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 172
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ATG12)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 333 AA; 36887 MW; 6C49DB4D6C6EEBE9 CRC64;
MASPTPAPSA IQRRVWEGRI PLEITLSPAE CRTYDKADPY LICYPRVSYL PFLLPRLRAF
FATSLIDIEA QDYQGWFSFE GVPLKWHYPL GLLYDLYSGA DPVTSKSTVG EHLSHTTSPR
QPNDPEPHNV HEPGDPIPWQ LQVHFSDWPD QELVRLDADG RVIHDAFINS VKEADFVRNG
TAKRIMTLSK EDSSGLWQAV QEHDFTNFQR ISNILIPGGP NHFRNIPLRI FLPSPPNSAT
PSLKVIQSLF PPTIPPAANQ TGAPGRQTQP QPQTIGSSLN SLLPSLFPSK RIPVLAKPVL
QGAVVPMTAP LEEVVRVAGY ADGWLAIVVS MVG