ATG5_ASPCL
ID ATG5_ASPCL Reviewed; 321 AA.
AC A1CE93;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Autophagy protein 5;
GN Name=atg5; ORFNames=ACLA_088830;
OS Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 /
OS NRRL 1 / QM 1276 / 107).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=344612;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
CC -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC autophagic vesicle formation. Autophagy is essential for maintenance of
CC amino acid levels and protein synthesis under nitrogen starvation.
CC Required for selective autophagic degradation of the nucleus
CC (nucleophagy). Also required for mitophagy, which eliminates defective
CC or superfluous mitochondria in order to fulfill cellular energy
CC requirements and prevent excess ROS production. Conjugation with atg12,
CC through a ubiquitin-like conjugating system involving atg7 as an E1-
CC like activating enzyme and atg10 as an E2-like conjugating enzyme, is
CC essential for its function. The atg12-atg5 conjugate acts as an E3-like
CC enzyme which is required for lipidation of atg8 and atg8 association to
CC the vesicle membranes (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Conjugated with atg12. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- PTM: Conjugated to atg12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAW11192.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DS027052; EAW11192.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001272618.1; XM_001272617.1.
DR AlphaFoldDB; A1CE93; -.
DR SMR; A1CE93; -.
DR STRING; 5057.CADACLAP00008623; -.
DR GeneID; 4704716; -.
DR KEGG; act:ACLA_088830; -.
DR eggNOG; KOG2976; Eukaryota.
DR OrthoDB; 457861at2759; -.
DR Proteomes; UP000006701; Unassembled WGS sequence.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..321
FT /note="Autophagy protein 5"
FT /id="PRO_0000317849"
FT CROSSLNK 157
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in atg12)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 321 AA; 35179 MW; FD387CE3BA4C3A0B CRC64;
MENQASLNSI QKTVWDGKLP LQITLASSES RTFDQTDPYL ISCPRISYLP SLLPRLRSFF
ASSLIEPKSQ PHEGWFSFEG VPLKWHLPIG LLYDLYAGAD PASKGSRPDE SEQIISSVGD
TLPWRLTLHF SDWPDEELVR LDADGMVMHD AFINSVKEAD FLRNGTAKGI MSLSKEDSSG
LWEAVQDVDL PSFQRISNIL LPAPNQPFRN VPIRFFLPLP PDSGSPSLKV VQSPLPPSIP
ASTANATQST VLRGKPASQL QTIGSALHSL LPNLFPSRRT PVLAKPVLHG AAVPMSAPVE
EVARSAAYGD GWVYIVVRMM G