PSAI_PEA
ID PSAI_PEA Reviewed; 40 AA.
AC P17227;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 2.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Photosystem I reaction center subunit VIII;
DE Short=PSI-I;
GN Name=psaI;
OS Pisum sativum (Garden pea).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Alaska;
RX PubMed=1807835; DOI=10.1007/bf00317074;
RA Nagano Y., Matsuno R., Sasaki Y.;
RT "Sequence and transcriptional analysis of the gene cluster trnQ-zfpA-psaI-
RT ORF231-petA in pea chloroplasts.";
RL Curr. Genet. 20:431-436(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Alaska;
RX PubMed=1913879; DOI=10.1007/bf00309603;
RA Smith A.G., Wilson R.J., Kaethner T.M., Willey D.L., Gray J.C.;
RT "Pea chloroplast genes encoding a 4 kDa polypeptide of photosystem I and a
RT putative enzyme of C1 metabolism.";
RL Curr. Genet. 19:403-410(1991).
RN [3]
RP PROTEIN SEQUENCE OF 1-16.
RX PubMed=2185953; DOI=10.1016/0014-5793(90)81391-z;
RA Ikeuchi M., Hirano A., Hiyama T., Inoue Y.;
RT "Polypeptide composition of higher plant photosystem I complex.
RT Identification of psaI, psaJ and psaK gene products.";
RL FEBS Lett. 263:274-278(1990).
RN [4]
RP PROTEIN SEQUENCE OF 3-25.
RX PubMed=8262226; DOI=10.1016/0014-5793(93)81617-9;
RA Zakharov S.D., Ewy R.G., Dilley R.A.;
RT "Subunit III of the chloroplast ATP-synthase can form a Ca(2+)-binding site
RT on the lumenal side of the thylakoid membrane.";
RL FEBS Lett. 336:95-99(1993).
CC -!- FUNCTION: May help in the organization of the PsaL subunit.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PsaI family. {ECO:0000305}.
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DR EMBL; X56315; CAA39757.1; -; Genomic_DNA.
DR EMBL; X54750; CAA38547.1; -; Genomic_DNA.
DR PIR; S17921; S17921.
DR RefSeq; YP_003587557.1; NC_014057.1.
DR PDB; 2O01; X-ray; 3.40 A; I=1-30.
DR PDB; 2WSC; X-ray; 3.30 A; I=1-40.
DR PDB; 2WSE; X-ray; 3.49 A; I=1-40.
DR PDB; 2WSF; X-ray; 3.48 A; I=1-40.
DR PDB; 3LW5; X-ray; 3.30 A; I=1-30.
DR PDB; 4RKU; X-ray; 3.00 A; I=5-30.
DR PDB; 4XK8; X-ray; 2.80 A; I/i=2-31.
DR PDB; 4Y28; X-ray; 2.80 A; I=3-32.
DR PDB; 5L8R; X-ray; 2.60 A; I=1-40.
DR PDB; 6LY5; EM; 2.38 A; i=1-40.
DR PDB; 6YAC; EM; 2.50 A; I=2-32.
DR PDB; 6YEZ; EM; 2.70 A; I=2-32.
DR PDB; 6ZOO; EM; 2.74 A; I=2-32.
DR PDB; 6ZXS; X-ray; 3.00 A; I=3-32.
DR PDB; 7DKZ; X-ray; 2.39 A; I=1-40.
DR PDBsum; 2O01; -.
DR PDBsum; 2WSC; -.
DR PDBsum; 2WSE; -.
DR PDBsum; 2WSF; -.
DR PDBsum; 3LW5; -.
DR PDBsum; 4RKU; -.
DR PDBsum; 4XK8; -.
DR PDBsum; 4Y28; -.
DR PDBsum; 5L8R; -.
DR PDBsum; 6LY5; -.
DR PDBsum; 6YAC; -.
DR PDBsum; 6YEZ; -.
DR PDBsum; 6ZOO; -.
DR PDBsum; 6ZXS; -.
DR PDBsum; 7DKZ; -.
DR AlphaFoldDB; P17227; -.
DR SMR; P17227; -.
DR DIP; DIP-60289N; -.
DR IntAct; P17227; 3.
DR GeneID; 9073045; -.
DR BRENDA; 1.97.1.12; 4872.
DR EvolutionaryTrace; P17227; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009522; C:photosystem I; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00431; PSI_PsaI; 1.
DR InterPro; IPR001302; PSI_PsaI.
DR InterPro; IPR036357; PSI_PsaI_sf.
DR PANTHER; PTHR35775; PTHR35775; 1.
DR Pfam; PF00796; PSI_8; 1.
DR SUPFAM; SSF81540; SSF81540; 1.
DR TIGRFAMs; TIGR03052; PS_I_psaI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Membrane;
KW Photosynthesis; Photosystem I; Plastid; Thylakoid; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..40
FT /note="Photosystem I reaction center subunit VIII"
FT /id="PRO_0000194668"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 13
FT /note="G -> S (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 22
FT /note="A -> R (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 5..13
FT /evidence="ECO:0007829|PDB:6LY5"
FT HELIX 15..28
FT /evidence="ECO:0007829|PDB:6LY5"
SQ SEQUENCE 40 AA; 4470 MW; 3CE0B06A11A58E48 CRC64;
MINLPSLFVP LVGLLFPAVA MASLFLHVEK RLLFSTKKIN