ATG5_DICDI
ID ATG5_DICDI Reviewed; 398 AA.
AC Q54GT9; Q86CS1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Autophagy protein 5;
GN Name=atg5; Synonyms=apg5; ORFNames=DDB_G0289881;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=AX3 / DH1;
RX PubMed=12626495; DOI=10.1074/jbc.m212467200;
RA Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT "Macroautophagy is required for multicellular development of the social
RT amoeba Dictyostelium discoideum.";
RL J. Biol. Chem. 278:17636-17645(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in autophagic vesicle formation. Conjugation with
CC atg12, through a ubiquitin-like conjugating system involving atg7 as an
CC E1-like activating enzyme and atg10 as an E2-like conjugating enzyme,
CC is essential for its function. The atg12-atg5 conjugate acts as an E3-
CC like enzyme which is required for lipidation of atg8 and its
CC association to the vesicle membranes (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:12626495}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Preautophagosomal
CC structure membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}.
CC -!- PTM: Conjugated to atg12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR EMBL; AY191012; AAO39075.1; -; Genomic_DNA.
DR EMBL; AAFI02000149; EAL62453.1; -; Genomic_DNA.
DR RefSeq; XP_635980.1; XM_630888.1.
DR AlphaFoldDB; Q54GT9; -.
DR SMR; Q54GT9; -.
DR STRING; 44689.DDB0191390; -.
DR PaxDb; Q54GT9; -.
DR EnsemblProtists; EAL62453; EAL62453; DDB_G0289881.
DR GeneID; 8627395; -.
DR KEGG; ddi:DDB_G0289881; -.
DR dictyBase; DDB_G0289881; atg5.
DR eggNOG; KOG2976; Eukaryota.
DR HOGENOM; CLU_051894_0_0_1; -.
DR InParanoid; Q54GT9; -.
DR OMA; IKWNFVN; -.
DR PhylomeDB; Q54GT9; -.
DR Reactome; R-DDI-1632852; Macroautophagy.
DR Reactome; R-DDI-8934903; Receptor Mediated Mitophagy.
DR PRO; PR:Q54GT9; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR GO; GO:0000045; P:autophagosome assembly; IBA:GO_Central.
DR GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR GO; GO:0006501; P:C-terminal protein lipidation; IBA:GO_Central.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IMP:dictyBase.
DR GO; GO:0042742; P:defense response to bacterium; IDA:dictyBase.
DR GO; GO:0016236; P:macroautophagy; IMP:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IMP:dictyBase.
DR GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IMP:dictyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0010468; P:regulation of gene expression; IMP:dictyBase.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR GO; GO:0048837; P:sorocarp sorus development; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasm; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..398
FT /note="Autophagy protein 5"
FT /id="PRO_0000327428"
FT REGION 99..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 292..321
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CROSSLNK 187
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ATG12)"
FT /evidence="ECO:0000250"
FT CONFLICT 88
FT /note="Y -> H (in Ref. 1; AAO39075)"
FT /evidence="ECO:0000305"
FT CONFLICT 147
FT /note="Missing (in Ref. 1; AAO39075)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 398 AA; 46061 MW; 6325767E2F1E13F2 CRC64;
MSSFDEDIKR SIWEGKIPIV FTLSPDDLTS HLSPSPYTLM APRNSYFPLI TSLVKDYFSS
STLVLLDEMW LEYRGIPLKW HLPIGVLYDT IVGNNNCNNS NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN NNNNNNNNNN NNNNNNNIIM EQPYWNIVVH FQSYPDRILL RCPNIESVRT
YYKNVLKEAN FIKQGDITKI NNLNINQSND LWDGLKSHDY DKFWSVNKKL IPNSNKEYKN
IPIRLIINYK PPIQELIPVF DENLVELTLE NLFSRIPYES FSNFLNYNNN NNNNNINNNS
PPLSPNSNNN NNNNNVDNSI ENSLNQTNVE SAEPEFSNLL QYIKATNAEY KIQGIQPSLK
SSAVWLYEHF GHPDNFLYIV LIDPSQNNNN NNNNSNNY
