ATG5_LODEL
ID ATG5_LODEL Reviewed; 286 AA.
AC A5DWA7;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Autophagy protein 5;
GN Name=ATG5; ORFNames=LELG_01643;
OS Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC
OS 1676 / NRRL YB-4239) (Yeast) (Saccharomyces elongisporus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade;
OC Lodderomyces.
OX NCBI_TaxID=379508;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 11503 / BCRC 21390 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL
RC YB-4239;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC autophagic vesicle formation. Autophagy is essential for maintenance of
CC amino acid levels and protein synthesis under nitrogen starvation.
CC Required for selective autophagic degradation of the nucleus
CC (nucleophagy). Also required for mitophagy, which eliminates defective
CC or superfluous mitochondria in order to fulfill cellular energy
CC requirements and prevent excess ROS production. Conjugation with ATG12,
CC through a ubiquitin-like conjugating system involving ATG7 as an E1-
CC like activating enzyme and ATG10 as an E2-like conjugating enzyme, is
CC essential for its function. The ATG12-ATG5 conjugate acts as an E3-like
CC enzyme which is required for lipidation of ATG8 and ATG8 association to
CC the vesicle membranes (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Conjugated with ATG12. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- PTM: Conjugated to ATG12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR EMBL; CH981525; EDK43465.1; -; Genomic_DNA.
DR RefSeq; XP_001526815.1; XM_001526765.1.
DR AlphaFoldDB; A5DWA7; -.
DR SMR; A5DWA7; -.
DR STRING; 379508.A5DWA7; -.
DR EnsemblFungi; EDK43465; EDK43465; LELG_01643.
DR GeneID; 5234272; -.
DR KEGG; lel:LELG_01643; -.
DR VEuPathDB; FungiDB:LELG_01643; -.
DR eggNOG; KOG2976; Eukaryota.
DR HOGENOM; CLU_051894_2_0_1; -.
DR InParanoid; A5DWA7; -.
DR OMA; HEGESPL; -.
DR OrthoDB; 457861at2759; -.
DR Proteomes; UP000001996; Unassembled WGS sequence.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..286
FT /note="Autophagy protein 5"
FT /id="PRO_0000317855"
FT CROSSLNK 160
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ATG12)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 286 AA; 32671 MW; 0367202868A3832F CRC64;
MSKLTSIEEN IEIKRRLWNG SISLKIIISI VGESSEDGIT NEYEYLTEGY RNAYLPTLYP
NIVSYIKKLP TVAFSNNSPI WLEYEGVPLR WNLPIGVLYD HLYLPAKFTN QSTPWTLDLK
IASSTLPYPS NYIVPFTYSA EDGSVNYTKS INEMLLNQLK QSCYVLNGTA KPIMQLNGPE
TELLCNLVIT RNLKTYNFFN NKIIKQIRGI PVRIYLPNTQ LVAQAAVDPH ILLQDLLLER
IPTLMEVSVP IIHGIDAGPL LKFQLNDIWH QFKHPDNVLY ISILMP