ID   ATG5_NEOFI              Reviewed;         326 AA.
AC   A1DMA1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Autophagy protein 5;
GN   Name=atg5; ORFNames=NFIA_052680;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC       autophagic vesicle formation. Autophagy is essential for maintenance of
CC       amino acid levels and protein synthesis under nitrogen starvation.
CC       Required for selective autophagic degradation of the nucleus
CC       (nucleophagy). Also required for mitophagy, which eliminates defective
CC       or superfluous mitochondria in order to fulfill cellular energy
CC       requirements and prevent excess ROS production. Conjugation with atg12,
CC       through a ubiquitin-like conjugating system involving atg7 as an E1-
CC       like activating enzyme and atg10 as an E2-like conjugating enzyme, is
CC       essential for its function. The atg12-atg5 conjugate acts as an E3-like
CC       enzyme which is required for lipidation of atg8 and atg8 association to
CC       the vesicle membranes (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Conjugated with atg12. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC   -!- PTM: Conjugated to atg12; which is essential for autophagy.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR   EMBL; DS027698; EAW15922.1; -; Genomic_DNA.
DR   RefSeq; XP_001257819.1; XM_001257818.1.
DR   AlphaFoldDB; A1DMA1; -.
DR   SMR; A1DMA1; -.
DR   STRING; 36630.CADNFIAP00004303; -.
DR   EnsemblFungi; EAW15922; EAW15922; NFIA_052680.
DR   GeneID; 4584334; -.
DR   KEGG; nfi:NFIA_052680; -.
DR   VEuPathDB; FungiDB:NFIA_052680; -.
DR   eggNOG; KOG2976; Eukaryota.
DR   HOGENOM; CLU_051894_2_0_1; -.
DR   OMA; KWHYPLG; -.
DR   OrthoDB; 457861at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.246.190; -; 1.
DR   Gene3D; 3.10.20.620; -; 1.
DR   InterPro; IPR007239; Atg5.
DR   InterPro; IPR042526; Atg5_HR.
DR   InterPro; IPR042527; Atg5_UblA_dom.
DR   PANTHER; PTHR13040; PTHR13040; 1.
DR   Pfam; PF04106; APG5; 1.
PE   3: Inferred from homology;
KW   Autophagy; Isopeptide bond; Membrane; Protein transport;
KW   Reference proteome; Transport; Ubl conjugation.
FT   CHAIN           1..326
FT                   /note="Autophagy protein 5"
FT                   /id="PRO_0000317856"
FT   CROSSLNK        163
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in atg12)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   326 AA;  35957 MW;  89CC3A6514809C91 CRC64;
     MRTSSRMENQ ASLSSIQRAV WDGKLPLQIT LASSESRTYD QTDPYLIACP RISYLPSLLP
     RLRAFFSSSL IEPNSQPHEG WFSFEGVPLK WHLPVGLLYD LYAGADPASK GTRIDETDHP
     SSSLSDTLPW RLTVHFSDWP DEELVRLDAD GMVMHDAFIN SVKEADFLRN GTAKGIMTLS
     KEDSAGLWQA VQDVDLPSFQ RISNILLPPP NQPFRNIPIR FFLPLSPDSG SPSLKVVQSP
     LPPSIPVATN TSQSTNLRNS PATQVQTLGS ALHSLLPNLF PSRRTPVLAK PVLHGAAVPM
     SAPIEELVRS CAYGDGWAYI VIRMMG