ATG5_PICAN
ID ATG5_PICAN Reviewed; 277 AA.
AC A7KAI4;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-SEP-2007, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Autophagy protein 5;
GN Name=ATG5;
OS Pichia angusta (Yeast) (Hansenula polymorpha).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Pichiaceae; Ogataea.
OX NCBI_TaxID=870730;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL
RC Y-5445;
RX PubMed=17204848; DOI=10.4161/auto.3595;
RA Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W.;
RT "ATG genes involved in non-selective autophagy are conserved from yeast to
RT man, but the selective Cvt and pexophagy pathways also require organism-
RT specific genes.";
RL Autophagy 3:106-116(2007).
CC -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC autophagic vesicle formation. Autophagy is essential for maintenance of
CC amino acid levels and protein synthesis under nitrogen starvation.
CC Required for selective autophagic degradation of the nucleus
CC (nucleophagy). Also required for mitophagy, which eliminates defective
CC or superfluous mitochondria in order to fulfill cellular energy
CC requirements and prevent excess ROS production. Conjugation with ATG12,
CC through a ubiquitin-like conjugating system involving ATG7 as an E1-
CC like activating enzyme and ATG10 as an E2-like conjugating enzyme, is
CC essential for its function. The ATG12-ATG5 conjugate acts as an E3-like
CC enzyme which is required for lipidation of ATG8 and ATG8 association to
CC the vesicle membranes (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:17204848}.
CC -!- SUBUNIT: Conjugated with ATG12. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- PTM: Conjugated to ATG12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR EMBL; EF102885; ABO31289.1; -; Genomic_DNA.
DR AlphaFoldDB; A7KAI4; -.
DR SMR; A7KAI4; -.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport; Transport;
KW Ubl conjugation.
FT CHAIN 1..277
FT /note="Autophagy protein 5"
FT /id="PRO_0000317859"
FT CROSSLNK 135
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ATG12)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 277 AA; 32128 MW; E2AEAFAF57BA3153 CRC64;
MSTSEIISRV WGGVLYMQFH LDRALSNQEC PSFYVAVHRN SYLHNSLPAI LQFFKPFLKD
ARLAQSQKWW FEFEKVPLKW NFPVGLLYDL VTTDAQVEKQ MWEITLKYYD YPIEYVIPID
QNPSFLKDHW TNQLKEACFI LNGSSKLVMN MSRTDSDDFY HAAIHKDSTQ FESMFRKLLP
SSVSSLKNLP IKVYLPLSNK LIQPVLSNLG RKITLGNLLQ DLIPDLFPSS LMYTVAHPYS
HGVVLPLDSS IIDLYICMKS LDGFLHISIK MIQKNEH