ATG5_PICGU
ID ATG5_PICGU Reviewed; 258 AA.
AC A5DCZ3;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 2.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Autophagy protein 5;
GN Name=ATG5; ORFNames=PGUG_01148;
OS Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539
OS / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Meyerozyma.
OX NCBI_TaxID=294746;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC autophagic vesicle formation. Autophagy is essential for maintenance of
CC amino acid levels and protein synthesis under nitrogen starvation.
CC Required for selective autophagic degradation of the nucleus
CC (nucleophagy). Also required for mitophagy, which eliminates defective
CC or superfluous mitochondria in order to fulfill cellular energy
CC requirements and prevent excess ROS production. Conjugation with ATG12,
CC through a ubiquitin-like conjugating system involving ATG7 as an E1-
CC like activating enzyme and ATG10 as an E2-like conjugating enzyme, is
CC essential for its function. The ATG12-ATG5 conjugate acts as an E3-like
CC enzyme which is required for lipidation of ATG8 and ATG8 association to
CC the vesicle membranes (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Conjugated with ATG12. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC -!- PTM: Conjugated to ATG12; which is essential for autophagy.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
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DR EMBL; CH408155; EDK37050.2; -; Genomic_DNA.
DR RefSeq; XP_001487771.1; XM_001487721.1.
DR AlphaFoldDB; A5DCZ3; -.
DR SMR; A5DCZ3; -.
DR STRING; 4929.XP_001487771.1; -.
DR EnsemblFungi; EDK37050; EDK37050; PGUG_01148.
DR GeneID; 5129564; -.
DR KEGG; pgu:PGUG_01148; -.
DR eggNOG; KOG2976; Eukaryota.
DR HOGENOM; CLU_051894_2_0_1; -.
DR InParanoid; A5DCZ3; -.
DR OMA; KWHYPLG; -.
DR OrthoDB; 457861at2759; -.
DR Proteomes; UP000001997; Unassembled WGS sequence.
DR GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 1.10.246.190; -; 1.
DR Gene3D; 3.10.20.620; -; 1.
DR InterPro; IPR007239; Atg5.
DR InterPro; IPR042526; Atg5_HR.
DR InterPro; IPR042527; Atg5_UblA_dom.
DR PANTHER; PTHR13040; PTHR13040; 1.
DR Pfam; PF04106; APG5; 1.
PE 3: Inferred from homology;
KW Autophagy; Isopeptide bond; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation.
FT CHAIN 1..258
FT /note="Autophagy protein 5"
FT /id="PRO_0000317860"
FT CROSSLNK 125
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ATG12)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 258 AA; 29888 MW; D15DDE01783B7E37 CRC64;
MPLEQDIKLH IWNGAINIVI VYNEQQYLVR AHRNSYLPLY YADISAYFEL SQPLWLEYQG
VPLKWNLPVG VLSDLLRTPS KEAWEVQLRT DNLPSHEIIP YHYHVEDNSI DYTRALREVV
INHIKQSCYT LNGNAKKIMG LSESESKRLW ISIATHNLPE YHQITSKILP SDIHRIPVKV
YLSGTSTVFN FAVDHSHQKP TTLRKVLGDL TPALLDENLA TTVLIHGVDC NSLIDEPILD
VWRTFRYLDN FLYIILMP