ID   ATG5_YEAS7              Reviewed;         294 AA.
AC   A6ZWA5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=Autophagy protein 5;
GN   Name=ATG5; ORFNames=SCY_5582;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Involved in cytoplasm to vacuole transport (Cvt) and
CC       autophagic vesicle formation. Autophagy is essential for maintenance of
CC       amino acid levels and protein synthesis under nitrogen starvation.
CC       Required for selective autophagic degradation of the nucleus
CC       (nucleophagy). Also required for mitophagy, which eliminates defective
CC       or superfluous mitochondria in order to fulfill cellular energy
CC       requirements and prevent excess ROS production. Conjugation with ATG12,
CC       through a ubiquitin-like conjugating system involving ATG7 as an E1-
CC       like activating enzyme and ATG10 as an E2-like conjugating enzyme, is
CC       essential for its function. The ATG12-ATG5 conjugate acts as an E3-like
CC       enzyme which is required for lipidation of ATG8 and ATG8 association to
CC       the vesicle membranes. ATG12-ATG5 rearranges the ATG3 catalytic center
CC       and enhances its E2 activity. Plays a role in the regulation of
CC       filamentous growth and chronological longevity (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Conjugated with ATG12. The ATG5-ATG12 conjugate forms a
CC       complex with several units of ATG16. The ATG12-ATG5 conjugate
CC       associates also with ATG3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC       Note=Localizes to the isolation membrane (IM), a membrane sac which is
CC       generated from the pre-autophagosomal structure (PAS). Ultimately, the
CC       IM expands to become a mature autophagosome. Localizes also to a dot at
CC       the junction between the IM and the vacuolar membrane, termed the
CC       vacuole-IM contact site (VICS). Correct localization to the PAS
CC       requires ATG21. {ECO:0000250}.
CC   -!- PTM: Conjugated to ATG12; which is essential for autophagy. Conjugation
CC       with ATG12 involves ATG7 as an E1-like activating enzyme and ATG10 as
CC       an E2-like conjugating enzyme (By similarity). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Small amount of ATG5-ATG12 conjugate is enough to
CC       perform normal autophagy. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ATG5 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAFW02000135; EDN60997.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZWA5; -.
DR   SMR; A6ZWA5; -.
DR   PRIDE; A6ZWA5; -.
DR   EnsemblFungi; EDN60997; EDN60997; SCY_5582.
DR   HOGENOM; CLU_051894_2_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.246.190; -; 1.
DR   Gene3D; 3.10.20.620; -; 1.
DR   InterPro; IPR007239; Atg5.
DR   InterPro; IPR042526; Atg5_HR.
DR   InterPro; IPR042527; Atg5_UblA_dom.
DR   PANTHER; PTHR13040; PTHR13040; 1.
DR   Pfam; PF04106; APG5; 1.
PE   3: Inferred from homology;
KW   Autophagy; Isopeptide bond; Membrane; Protein transport; Transport;
KW   Ubl conjugation.
FT   CHAIN           1..294
FT                   /note="Autophagy protein 5"
FT                   /id="PRO_0000317864"
FT   CROSSLNK        149
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in ATG12)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   294 AA;  33594 MW;  EE513A604B31CD10 CRC64;
     MNDIKQLLWN GELNVLVSID PSFLMKGSPR EIAVLRIRVP RETYLVNYMP FIWNKIKSFL
     SFDPLTDSEK YFWFEHNKTP IPWNYPVGVL FDCLAGKSAT FTTSFENQVK DVLTFLRIHL
     VMGDSLPPTI IPIASSKTQA EKFWFHQWKQ VCFILNGSSK AIMSLSVNEA RKFWGSVITR
     NFQDFIEISN KISSSRPRHI PLIIQTSRTS GTFRISQPTI SMTGVNPTLK DIEGDILDVK
     EGINGNDVMV ICQGIEIPWH MLLYDLYSKL RSFDGFLYIT LVPIKGGDKA SSEL