PSAN_ARATH
ID PSAN_ARATH Reviewed; 171 AA.
AC P49107; Q2V2V9;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Photosystem I reaction center subunit N, chloroplastic;
DE Short=PSI-N;
DE Flags: Precursor;
GN Name=PSAN; OrderedLocusNames=At5g64040; ORFNames=MHJ24.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Sehnke P.C., Ferl R.J.;
RT "Nucleotide sequence of an Arabidopsis thaliana cDNA clone encoding the
RT complete precursor for a homolog to the barley extrinsic thylakoid lumenal
RT polypeptide PSI-N.";
RL (er) Plant Gene Register PGR95-088(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11826309; DOI=10.1105/tpc.010304;
RA Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA van Wijk K.J.;
RT "Central functions of the lumenal and peripheral thylakoid proteome of
RT Arabidopsis determined by experimentation and genome-wide prediction.";
RL Plant Cell 14:211-236(2002).
RN [6]
RP FUNCTION.
RX PubMed=10230065; DOI=10.1046/j.1365-313x.1999.00419.x;
RA Haldrup A., Naver H., Scheller H.V.;
RT "The interaction between plastocyanin and photosystem I is inefficient in
RT transgenic Arabidopsis plants lacking the PSI-N subunit of photosystem I.";
RL Plant J. 17:689-698(1999).
RN [7]
RP INTERACTION WITH 14-3-3-LIKE PROTEINS.
RX PubMed=10631267; DOI=10.1104/pp.122.1.235;
RA Sehnke P.C., Henry R., Cline K., Ferl R.J.;
RT "Interaction of a plant 14-3-3 protein with the signal peptide of a
RT thylakoid-targeted chloroplast precursor protein and the presence of 14-3-3
RT isoforms in the chloroplast stroma.";
RL Plant Physiol. 122:235-242(2000).
RN [8]
RP FUNCTION.
RX PubMed=12324436; DOI=10.1016/s0006-3495(02)73979-9;
RA Ihalainen J.A., Jensen P.E., Haldrup A., van Stokkum I.H.M.,
RA van Grondelle R., Scheller H.V., Dekker J.P.;
RT "Pigment organization and energy transfer dynamics in isolated photosystem
RT I (PSI) complexes from Arabidopsis thaliana depleted of the PSI-G, PSI-K,
RT PSI-L, or PSI-N subunit.";
RL Biophys. J. 83:2190-2201(2002).
RN [9]
RP ACETYLATION AT GLY-87, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR
RP LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: May function in mediating the binding of the antenna
CC complexes to the PSI reaction center and core antenna. Plays an
CC important role in docking plastocyanin to the PSI complex. Does not
CC bind pigments. {ECO:0000269|PubMed:10230065,
CC ECO:0000269|PubMed:12324436}.
CC -!- SUBUNIT: Interacts with GRF5, GRF7, GRF9 and GRF10. The transit peptide
CC is the site of PSAN that associates with 14-3-3.
CC {ECO:0000269|PubMed:10631267}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:18431481}; Peripheral membrane protein; Lumenal
CC side.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P49107-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P49107-2; Sequence=VSP_034386, VSP_034387;
CC -!- MISCELLANEOUS: The N-terminal Edman sequence is 2 amino acids down-
CC stream of the N-acetylated terminal peptide identified by mass
CC spectrometry. This suggests an additional processing.
CC -!- SIMILARITY: Belongs to the psaN family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U32176; AAA93075.1; -; mRNA.
DR EMBL; AB008266; BAB10272.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97832.1; -; Genomic_DNA.
DR EMBL; AY062835; AAL32913.1; -; mRNA.
DR EMBL; AY081594; AAM10156.1; -; mRNA.
DR RefSeq; NP_201209.1; NM_125800.5. [P49107-1]
DR AlphaFoldDB; P49107; -.
DR SMR; P49107; -.
DR BioGRID; 21767; 3.
DR IntAct; P49107; 1.
DR STRING; 3702.AT5G64040.2; -.
DR iPTMnet; P49107; -.
DR PaxDb; P49107; -.
DR PRIDE; P49107; -.
DR ProteomicsDB; 226395; -. [P49107-1]
DR EnsemblPlants; AT5G64040.1; AT5G64040.1; AT5G64040. [P49107-1]
DR GeneID; 836525; -.
DR Gramene; AT5G64040.1; AT5G64040.1; AT5G64040. [P49107-1]
DR KEGG; ath:AT5G64040; -.
DR Araport; AT5G64040; -.
DR eggNOG; ENOG502S2VP; Eukaryota.
DR HOGENOM; CLU_113897_1_0_1; -.
DR InParanoid; P49107; -.
DR OMA; ACNYARS; -.
DR PhylomeDB; P49107; -.
DR BioCyc; MetaCyc:MON-1095; -.
DR PRO; PR:P49107; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P49107; baseline and differential.
DR Genevisible; P49107; AT.
DR GO; GO:0030093; C:chloroplast photosystem I; IBA:GO_Central.
DR GO; GO:0019904; F:protein domain specific binding; IPI:CAFA.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 4.10.1190.10; -; 1.
DR InterPro; IPR008796; PSAN.
DR InterPro; IPR044907; PSAN_sf.
DR PANTHER; PTHR36814; PTHR36814; 1.
DR Pfam; PF05479; PsaN; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Chloroplast; Direct protein sequencing;
KW Membrane; Photosynthesis; Photosystem I; Plastid; Reference proteome;
KW Thylakoid; Transit peptide.
FT TRANSIT 1..49
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 50..86
FT /note="Thylakoid"
FT /evidence="ECO:0000255"
FT CHAIN 87..171
FT /note="Photosystem I reaction center subunit N,
FT chloroplastic"
FT /id="PRO_0000029356"
FT MOD_RES 87
FT /note="N-acetylglycine; partial"
FT /evidence="ECO:0000269|PubMed:18431481"
FT VAR_SEQ 143..155
FT /note="KVPFISEDIALEC -> VCLISTTFSFLWE (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034386"
FT VAR_SEQ 156..171
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_034387"
SQ SEQUENCE 171 AA; 18429 MW; 82FE2AFF7C599C07 CRC64;
MAAMNSSVLT CSYAIAGSGS VELNQKVGLV NSSVGFGQKK QMIMPVIKAQ RVVGDDVDGS
NGRRSAMVFL AATLFSTAAV SASANAGVID EYLERSKTNK ELNDKKRLAT SGANFARAFT
VQFGSCKFPE NFTGCQDLAK QKKVPFISED IALECEGKDK YKCGSNVFWK W
