PSAO_ARATH
ID PSAO_ARATH Reviewed; 140 AA.
AC Q949Q5; Q8L7C3;
DT 03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Photosystem I subunit O;
DE Short=PSI-O;
DE Flags: Precursor;
GN Name=PSAO; OrderedLocusNames=At1g08380; ORFNames=T27G7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 52-67, FUNCTION, SUBUNIT,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=11801243; DOI=10.1016/s0014-5793(01)03253-7;
RA Knoetzel J., Mant A., Haldrup A., Jensen P.E., Scheller H.V.;
RT "PSI-O, a new 10-kDa subunit of eukaryotic photosystem I.";
RL FEBS Lett. 510:145-148(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION WITH PSAL.
RC STRAIN=cv. Columbia;
RX PubMed=15169790; DOI=10.1074/jbc.m403147200;
RA Jensen P.E., Haldrup A., Zhang S., Scheller H.V.;
RT "The PSI-O subunit of plant photosystem I is involved in balancing the
RT excitation pressure between the two photosystems.";
RL J. Biol. Chem. 279:24212-24217(2004).
CC -!- FUNCTION: Involved in the balancing of excitation energy between the
CC two photosystems I (PSI) and II (PSII). {ECO:0000269|PubMed:11801243,
CC ECO:0000269|PubMed:15169790}.
CC -!- SUBUNIT: Component of the photosystem I (PSI) complex. Interacts
CC directly with PSAL. {ECO:0000269|PubMed:11801243,
CC ECO:0000269|PubMed:15169790}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:11801243}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11801243}. Note=Prevalent in stroma lamellae with
CC low amounts in grana.
CC -!- DISRUPTION PHENOTYPE: Reduction by 50 percent in state transitions due
CC to a disturbed balancing of excitation energy between the two
CC photosystems. Short flowering delay. {ECO:0000269|PubMed:15169790}.
CC -!- SIMILARITY: Belongs to the PSAO family. {ECO:0000305}.
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DR EMBL; AJ493060; CAD37939.1; -; mRNA.
DR EMBL; AC006932; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE28283.1; -; Genomic_DNA.
DR EMBL; AY050960; AAK93637.1; -; mRNA.
DR EMBL; AY091345; AAM14284.1; -; mRNA.
DR EMBL; AY136345; AAM97011.1; -; mRNA.
DR EMBL; BT000191; AAN15510.1; -; mRNA.
DR EMBL; AY087368; AAM64918.1; -; mRNA.
DR RefSeq; NP_563815.1; NM_100711.4.
DR AlphaFoldDB; Q949Q5; -.
DR SMR; Q949Q5; -.
DR BioGRID; 22599; 1.
DR STRING; 3702.AT1G08380.1; -.
DR PaxDb; Q949Q5; -.
DR PRIDE; Q949Q5; -.
DR ProteomicsDB; 226326; -.
DR EnsemblPlants; AT1G08380.1; AT1G08380.1; AT1G08380.
DR GeneID; 837358; -.
DR Gramene; AT1G08380.1; AT1G08380.1; AT1G08380.
DR KEGG; ath:AT1G08380; -.
DR Araport; AT1G08380; -.
DR TAIR; locus:2201836; AT1G08380.
DR eggNOG; ENOG502S09E; Eukaryota.
DR HOGENOM; CLU_151524_0_0_1; -.
DR InParanoid; Q949Q5; -.
DR OMA; AVTSQFW; -.
DR OrthoDB; 1576725at2759; -.
DR PhylomeDB; Q949Q5; -.
DR BioCyc; MetaCyc:AT1G08380-MON; -.
DR PRO; PR:Q949Q5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q949Q5; baseline and differential.
DR Genevisible; Q949Q5; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009522; C:photosystem I; IDA:TAIR.
DR GO; GO:0009579; C:thylakoid; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0009768; P:photosynthesis, light harvesting in photosystem I; IC:TAIR.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IMP:UniProtKB.
DR InterPro; IPR017498; PSI_PsaO.
DR PANTHER; PTHR36311; PTHR36311; 1.
DR TIGRFAMs; TIGR03059; psaOeuk; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Membrane; Photosynthesis;
KW Photosystem I; Plastid; Reference proteome; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..51
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:11801243"
FT CHAIN 52..140
FT /note="Photosystem I subunit O"
FT /id="PRO_0000430153"
FT TOPO_DOM 52..60
FT /note="Stromal"
FT TRANSMEM 61..81
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..109
FT /note="Lumenal, thylakoid"
FT TRANSMEM 110..130
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..140
FT /note="Stromal"
FT CONFLICT 2
FT /note="A -> V (in Ref. 4; AAM97011/AAN15510)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 140 AA; 15144 MW; 4BBDFCB2A4D3EA90 CRC64;
MAATFATPST VIGLGGSSIT TKPFSSSFLK PTLSAKNPLR LAGASGGRVT CFERNWLRRD
LNVVGFGLIG WLAPSSIPAI NGKSLTGLFF DSIGTELAHF PTPPALTSQF WLWLVTWHLG
LFLCLTFGQI GFKGRTEDYF
