PSA_ORYSJ
ID PSA_ORYSJ Reviewed; 887 AA.
AC B7EA73; Q0J3M9; Q6ZBX8;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 10-FEB-2009, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Puromycin-sensitive aminopeptidase;
DE Short=PSA;
DE EC=3.4.11.14;
DE AltName: Full=Cytosol alanyl aminopeptidase;
DE Short=AAP-S;
GN OrderedLocusNames=Os08g0562700, LOC_Os08g44860; ORFNames=P0543D10.15;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal amino acid, preferentially alanine,
CC from a wide range of peptides, amides and arylamides.; EC=3.4.11.14;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the peptidase M1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD09617.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF24436.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004587; BAD09617.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008214; BAF24436.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK064900; BAG89270.1; -; mRNA.
DR RefSeq; XP_015649851.1; XM_015794365.1.
DR AlphaFoldDB; B7EA73; -.
DR SMR; B7EA73; -.
DR STRING; 4530.OS08T0562700-01; -.
DR MEROPS; M01.005; -.
DR PaxDb; B7EA73; -.
DR PRIDE; B7EA73; -.
DR GeneID; 4346337; -.
DR KEGG; osa:4346337; -.
DR eggNOG; KOG1046; Eukaryota.
DR OrthoDB; 110058at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.390.10; -; 1.
DR Gene3D; 1.25.50.10; -; 1.
DR Gene3D; 2.60.40.1730; -; 1.
DR Gene3D; 2.60.40.1840; -; 1.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR038438; PepN_Ig-like_sf.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR012779; Peptidase_M1_pepN.
DR InterPro; IPR024601; Peptidase_M1_pepN_C.
DR InterPro; IPR037144; Peptidase_M1_pepN_C_sf.
DR InterPro; IPR035414; Peptidase_M1_pepN_Ig-like.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR46322; PTHR46322; 1.
DR Pfam; PF11940; DUF3458; 1.
DR Pfam; PF17432; DUF3458_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; SSF63737; 1.
DR TIGRFAMs; TIGR02414; pepN_proteo; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 2: Evidence at transcript level;
KW Aminopeptidase; Hydrolase; Metal-binding; Metalloprotease; Protease;
KW Reference proteome; Zinc.
FT CHAIN 1..887
FT /note="Puromycin-sensitive aminopeptidase"
FT /id="PRO_0000424591"
FT ACT_SITE 301
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 124
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 264..268
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 323
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT SITE 384
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250"
SQ SEQUENCE 887 AA; 99524 MW; AF188B1F7A26808E CRC64;
MDLPKEIFLK EYKKPDYLFD SVNLEFQLGE DKTIVTSKIA VSPGTEGTSS PLTLHGRDLK
LLSIKVNGKD LKSEDYMVDS RHLTVSRPPG GTFNLEIVTE IYPQLNTSLE GLYKSTGNFC
TQCEAEGFRK ITYFQDRPDV MATYTCRIEA DKTLYPVLLS NGNLIEQGDL EGGKHYALWE
DPFKKPSYLF ALVAGQLDCR EDSFTTCSGR KVTLRIWTPG QDLAKTAHAM YSLKAAMKWD
EEVFGLEYDL DLFNIVVVPD FNMGAMENKS LNIFQSRLVL ASPETATDGD YAAILGVVGH
EYFHNWTGNR VTCRDWFQLT LKEGLTVFRD QEFSSDLGCR TVKRIADVSK LRTYQFPQDA
GPMAHPIRPH SYIKMDNFYT VTVYEKGAEV VRMYKTMFGA SGFRKGMDLY FQRHDGQAVT
CEDFYAAMCD ANNTQLPNFL QWYSQAGTPT VKVSSSYDAS SQTFSLKFSQ EVPPTPGQPV
KEPMFIPIAV GLVDSTGKDM PLTSIYSDGM LQSLTSDGQP VFTTVLQFNK KEEEFIFNNI
PEKPVPSLLR GYSAPVRLDS DLTESDLFFL LANDSDEFNR WEAGQVLSRK LMLSLVADFQ
QQKTLALNPK FVDGLRSILR NTSLDKEFIA KAITLPGQGE IMDMMPVADP DAVHAVRTFI
KKELALQLKD DLLSTVTNNR SSEAYTFNHD SMARRALKNT CLAYLASLNE PDTTELAFVE
YKSATNMTEQ FAALAALSQN PGQVRDDTLL DFYNKWQHDY LVVSKWFALQ ATSDIPGNVA
NVQKLLGHPA FDMRNPNKVY SLIGGFCGSP VNFHAKDGSG YKFLGEVVLQ LDKINPQVAS
RMVSAFSRWR RYDESRQALA KAQLEMIVSA NGLSENVYEI ASKSLAA
