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ATG8A_ARATH
ID   ATG8A_ARATH             Reviewed;         122 AA.
AC   Q8LEM4; O65447; Q8S928;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Autophagy-related protein 8a;
DE   AltName: Full=Autophagy-related ubiquitin-like modifier ATG8a;
DE            Short=AtAPG8a;
DE            Short=Protein autophagy 8a;
DE   Flags: Precursor;
GN   Name=ATG8A; Synonyms=APG8A; OrderedLocusNames=At4g21980; ORFNames=F1N20.80;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=12070171; DOI=10.1074/jbc.m204630200;
RA   Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.;
RT   "The APG8/12-activating enzyme APG7 is required for proper nutrient
RT   recycling and senescence in Arabidopsis thaliana.";
RL   J. Biol. Chem. 277:33105-33114(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, AND GENE FAMILY.
RX   PubMed=12114572; DOI=10.1104/pp.011024;
RA   Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D.,
RA   Tabata S., Ohsumi Y.;
RT   "Leaf senescence and starvation-induced chlorosis are accelerated by the
RT   disruption of an Arabidopsis autophagy gene.";
RL   Plant Physiol. 129:1181-1193(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   INTERACTION WITH ATG4B, AND BINDING TO MICROTUBULES.
RX   PubMed=15178341; DOI=10.1016/j.febslet.2004.04.088;
RA   Ketelaar T., Voss C., Dimmock S.A., Thumm M., Hussey P.J.;
RT   "Arabidopsis homologues of the autophagy protein Atg8 are a novel family of
RT   microtubule binding proteins.";
RL   FEBS Lett. 567:302-306(2004).
RN   [8]
RP   TISSUE SPECIFICITY.
RX   PubMed=15494556; DOI=10.1105/tpc.104.025395;
RA   Yoshimoto K., Hanaoka H., Sato S., Kato T., Tabata S., Noda T., Ohsumi Y.;
RT   "Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by
RT   ATG4s are essential for plant autophagy.";
RL   Plant Cell 16:2967-2983(2004).
RN   [9]
RP   INDUCTION.
RX   PubMed=16157655; DOI=10.1093/jxb/eri276;
RA   Slavikova S., Shy G., Yao Y., Glozman R., Levanony H., Pietrokovski S.,
RA   Elazar Z., Galili G.;
RT   "The autophagy-associated Atg8 gene family operates both under favourable
RT   growth conditions and under starvation stresses in Arabidopsis plants.";
RL   J. Exp. Bot. 56:2839-2849(2005).
RN   [10]
RP   INTERACTION WITH NBR1.
RX   PubMed=21606687; DOI=10.4161/auto.7.9.16389;
RA   Svenning S., Lamark T., Krause K., Johansen T.;
RT   "Plant NBR1 is a selective autophagy substrate and a functional hybrid of
RT   the mammalian autophagic adapters NBR1 and p62/SQSTM1.";
RL   Autophagy 7:993-1010(2011).
CC   -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC       May mediate the delivery of the autophagosomes to the vacuole via the
CC       microtubule cytoskeleton. {ECO:0000250|UniProtKB:P38182}.
CC   -!- SUBUNIT: Interacts with ATG4B (PubMed:15178341). Interacts with NBR1
CC       (PubMed:21606687). {ECO:0000269|PubMed:15178341,
CC       ECO:0000269|PubMed:21606687}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC       {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC       {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC       {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC       {ECO:0000250|UniProtKB:P38182}. Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:15178341}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q8LEM4-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Constitutively expressed.
CC       {ECO:0000269|PubMed:15494556}.
CC   -!- INDUCTION: Induced by sugar starvation. {ECO:0000269|PubMed:16157655}.
CC   -!- PTM: The C-terminal 5 residues are removed by ATG4 to expose Gly-117 at
CC       the C-terminus. This Gly-117 forms then a thioester bond with the 'Cys-
CC       558' of ATG7 (E1-like activating enzyme) before being transferred to
CC       the 'Cys-258' of ATG3 (the specific E2 conjugating enzyme), in order to
CC       be finally amidated with phosphatidylethanolamine. This lipid
CC       modification anchors ATG8 to autophagosomes.
CC       {ECO:0000250|UniProtKB:P38182}.
CC   -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA18101.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CAB79153.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF492759; AAM70188.1; -; mRNA.
DR   EMBL; AB073175; BAB88387.1; -; mRNA.
DR   EMBL; AL022140; CAA18101.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161556; CAB79153.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002687; AEE84537.1; -; Genomic_DNA.
DR   EMBL; BT002024; AAN72035.1; -; mRNA.
DR   EMBL; BT006522; AAP21330.1; -; mRNA.
DR   EMBL; AY085349; AAM62580.1; -; mRNA.
DR   PIR; T49105; T49105.
DR   RefSeq; NP_567642.1; NM_118319.4. [Q8LEM4-1]
DR   AlphaFoldDB; Q8LEM4; -.
DR   BMRB; Q8LEM4; -.
DR   SMR; Q8LEM4; -.
DR   BioGRID; 13576; 5.
DR   STRING; 3702.AT4G21980.2; -.
DR   PaxDb; Q8LEM4; -.
DR   PRIDE; Q8LEM4; -.
DR   EnsemblPlants; AT4G21980.1; AT4G21980.1; AT4G21980. [Q8LEM4-1]
DR   GeneID; 828287; -.
DR   Gramene; AT4G21980.1; AT4G21980.1; AT4G21980. [Q8LEM4-1]
DR   KEGG; ath:AT4G21980; -.
DR   Araport; AT4G21980; -.
DR   eggNOG; KOG1654; Eukaryota.
DR   HOGENOM; CLU_119276_0_1_1; -.
DR   InParanoid; Q8LEM4; -.
DR   PhylomeDB; Q8LEM4; -.
DR   PRO; PR:Q8LEM4; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q8LEM4; baseline and differential.
DR   Genevisible; Q8LEM4; AT.
DR   GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR   GO; GO:0006995; P:cellular response to nitrogen starvation; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR004241; Atg8-like.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   PANTHER; PTHR10969; PTHR10969; 1.
DR   Pfam; PF02991; ATG8; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Autophagy; Cytoplasm; Cytoplasmic vesicle;
KW   Cytoskeleton; Lipoprotein; Membrane; Microtubule; Protein transport;
KW   Reference proteome; Transport; Ubl conjugation pathway; Vacuole.
FT   CHAIN           1..117
FT                   /note="Autophagy-related protein 8a"
FT                   /id="PRO_0000286905"
FT   PROPEP          118..122
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250|UniProtKB:Q2XPP5"
FT                   /id="PRO_0000286906"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            117..118
FT                   /note="Cleavage; by ATG4"
FT                   /evidence="ECO:0000250|UniProtKB:Q2XPP5"
FT   LIPID           117
FT                   /note="Phosphatidylethanolamine amidated glycine"
FT                   /evidence="ECO:0000250|UniProtKB:P38182"
SQ   SEQUENCE   122 AA;  13695 MW;  9F04845BD559349B CRC64;
     MAKSSFKISN PLEARMSESS RIREKYPDRI PVIVEKAGQS DVPDIDKKKY LVPADLTVGQ
     FVYVVRKRIK LGAEKAIFVF VKNTLPPTAA LMSAIYEEHK DEDGFLYMTY SGENTFGSLT
     VA
 
 
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