ATG8A_ORYSI
ID ATG8A_ORYSI Reviewed; 119 AA.
AC Q2XPP5;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Autophagy-related protein 8A;
DE AltName: Full=Autophagy-related ubiquitin-like modifier ATG8A;
DE Short=Protein autophagy 8;
DE AltName: Full=OsAtg8;
DE Flags: Precursor;
GN Name=ATG8A; Synonyms=APG8, APG8A; ORFNames=OsI_025264;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH
RP ATG4, AND MUTAGENESIS OF GLY-117.
RX PubMed=17082902; DOI=10.1007/s11033-006-9011-0;
RA Su W., Ma H., Liu C., Wu J., Yang J.;
RT "Identification and characterization of two rice autophagy associated
RT genes, OsAtg8 and OsAtg4.";
RL Mol. Biol. Rep. 33:273-278(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Ubiquitin-like modifier involved in cytoplasm to vacuole
CC transport (Cvt) vesicles and autophagosomes formation. May mediate the
CC delivery of the vesicles and autophagosomes to the vacuole via the
CC microtubule cytoskeleton (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:17082902}.
CC -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC May mediate the delivery of the autophagosomes to the vacuole via the
CC microtubule cytoskeleton. {ECO:0000250|UniProtKB:P38182}.
CC -!- SUBUNIT: Interacts with ATG4. {ECO:0000269|PubMed:17082902}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q8LEM4}.
CC -!- TISSUE SPECIFICITY: Constitutively expressed.
CC {ECO:0000269|PubMed:17082902}.
CC -!- PTM: The C-terminal 2 residues are removed by ATG4 to expose Gly-117 at
CC the C-terminus. The C-terminal Gly is then amidated with
CC phosphatidylethanolamine by an activating system similar to that for
CC ubiquitin. {ECO:0000250|UniProtKB:P38182}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; DQ269983; ABB77258.1; -; mRNA.
DR EMBL; CM000132; EAZ04032.1; -; Genomic_DNA.
DR AlphaFoldDB; Q2XPP5; -.
DR SMR; Q2XPP5; -.
DR STRING; 39946.Q2XPP5; -.
DR EnsemblPlants; BGIOSGA024235-TA; BGIOSGA024235-PA; BGIOSGA024235.
DR Gramene; BGIOSGA024235-TA; BGIOSGA024235-PA; BGIOSGA024235.
DR HOGENOM; CLU_119276_0_1_1; -.
DR OMA; AHDQDIS; -.
DR Proteomes; UP000007015; Chromosome 7.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Lipoprotein;
KW Membrane; Microtubule; Protein transport; Reference proteome; Transport;
KW Ubl conjugation pathway; Vacuole.
FT CHAIN 1..117
FT /note="Autophagy-related protein 8A"
FT /id="PRO_0000286921"
FT PROPEP 118..119
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:17082902"
FT /id="PRO_0000286922"
FT SITE 117..118
FT /note="Cleavage; by ATG4"
FT /evidence="ECO:0000305|PubMed:17082902"
FT LIPID 117
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT MUTAGEN 117
FT /note="G->A: Abrogation of its C-terminal cleavage by
FT ATG4."
FT /evidence="ECO:0000269|PubMed:17082902"
SQ SEQUENCE 119 AA; 13664 MW; 1224DF7DD0CEF6A1 CRC64;
MARTSFKLEH PLERRQAESA RIREKYSDRI PVIVEKADKT DVPEIDKKKY LVPADLTVGQ
FVYVVRKRIK LSPEKAIFVF VKNTLPPTAS LMSAIYEENK DEDGFLYMTY SGENTFGSA
