ATG8I_ARATH
ID ATG8I_ARATH Reviewed; 115 AA.
AC Q9LRP7;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Autophagy-related protein 8i;
DE AltName: Full=Autophagy-related ubiquitin-like modifier ATG8i;
DE Short=AtAPG8i;
DE Short=Protein autophagy 8i;
GN Name=ATG8I; Synonyms=APG8I; OrderedLocusNames=At3g15580; ORFNames=MQD17.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12070171; DOI=10.1074/jbc.m204630200;
RA Doelling J.H., Walker J.M., Friedman E.M., Thompson A.R., Vierstra R.D.;
RT "The APG8/12-activating enzyme APG7 is required for proper nutrient
RT recycling and senescence in Arabidopsis thaliana.";
RL J. Biol. Chem. 277:33105-33114(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], NOMENCLATURE, AND GENE FAMILY.
RX PubMed=12114572; DOI=10.1104/pp.011024;
RA Hanaoka H., Noda T., Shirano Y., Kato T., Hayashi H., Shibata D.,
RA Tabata S., Ohsumi Y.;
RT "Leaf senescence and starvation-induced chlorosis are accelerated by the
RT disruption of an Arabidopsis autophagy gene.";
RL Plant Physiol. 129:1181-1193(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=15494556; DOI=10.1105/tpc.104.025395;
RA Yoshimoto K., Hanaoka H., Sato S., Kato T., Tabata S., Noda T., Ohsumi Y.;
RT "Processing of ATG8s, ubiquitin-like proteins, and their deconjugation by
RT ATG4s are essential for plant autophagy.";
RL Plant Cell 16:2967-2983(2004).
RN [8]
RP INTERACTION WITH NBR1.
RX PubMed=21606687; DOI=10.4161/auto.7.9.16389;
RA Svenning S., Lamark T., Krause K., Johansen T.;
RT "Plant NBR1 is a selective autophagy substrate and a functional hybrid of
RT the mammalian autophagic adapters NBR1 and p62/SQSTM1.";
RL Autophagy 7:993-1010(2011).
CC -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC May mediate the delivery of the autophagosomes to the vacuole via the
CC microtubule cytoskeleton. {ECO:0000250|UniProtKB:P38182}.
CC -!- SUBUNIT: Interacts with ATG4 (By similarity). Interacts with NBR1
CC (PubMed:21606687). {ECO:0000250|UniProtKB:Q9SL04,
CC ECO:0000269|PubMed:21606687}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:Q8LEM4}.
CC -!- TISSUE SPECIFICITY: Constitutively expressed.
CC {ECO:0000269|PubMed:15494556}.
CC -!- PTM: Gly-115 forms then a thioester bond with the 'Cys-558' of ATG7
CC (E1-like activating enzyme) before being transferred to the 'Cys-258'
CC of ATG3 (the specific E2 conjugating enzyme), in order to be finally
CC amidated with phosphatidylethanolamine. This lipid modification anchors
CC ATG8 to autophagosomes. {ECO:0000250|UniProtKB:P38182}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; AF492760; AAM70189.1; -; mRNA.
DR EMBL; AB073183; BAB88395.1; -; mRNA.
DR EMBL; AB028619; BAB01347.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75695.1; -; Genomic_DNA.
DR EMBL; AY086155; AAM63360.1; -; mRNA.
DR EMBL; BT024554; ABD38893.1; -; mRNA.
DR RefSeq; NP_566518.1; NM_112426.4.
DR AlphaFoldDB; Q9LRP7; -.
DR SMR; Q9LRP7; -.
DR BioGRID; 6132; 1.
DR IntAct; Q9LRP7; 1.
DR STRING; 3702.AT3G15580.1; -.
DR PaxDb; Q9LRP7; -.
DR PRIDE; Q9LRP7; -.
DR ProteomicsDB; 246741; -.
DR EnsemblPlants; AT3G15580.1; AT3G15580.1; AT3G15580.
DR GeneID; 820798; -.
DR Gramene; AT3G15580.1; AT3G15580.1; AT3G15580.
DR KEGG; ath:AT3G15580; -.
DR Araport; AT3G15580; -.
DR TAIR; locus:2092560; AT3G15580.
DR eggNOG; KOG1654; Eukaryota.
DR HOGENOM; CLU_119276_0_1_1; -.
DR InParanoid; Q9LRP7; -.
DR OMA; EFSHDER; -.
DR OrthoDB; 1508198at2759; -.
DR PhylomeDB; Q9LRP7; -.
DR PRO; PR:Q9LRP7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LRP7; baseline and differential.
DR Genevisible; Q9LRP7; AT.
DR GO; GO:0005776; C:autophagosome; IDA:TAIR.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0019779; F:Atg8 activating enzyme activity; ISS:TAIR.
DR GO; GO:0019776; F:Atg8 ligase activity; ISS:TAIR.
DR GO; GO:0019786; F:Atg8-specific peptidase activity; ISS:TAIR.
DR GO; GO:0006914; P:autophagy; ISS:TAIR.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Lipoprotein;
KW Membrane; Microtubule; Protein transport; Reference proteome; Transport;
KW Ubl conjugation pathway; Vacuole.
FT CHAIN 1..115
FT /note="Autophagy-related protein 8i"
FT /id="PRO_0000286920"
FT LIPID 115
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
SQ SEQUENCE 115 AA; 13251 MW; 854DCEA4AEDCA6F3 CRC64;
MKSFKEQYTL DERLAESREI IAKYPTRIPV IAEKYCKTDL PAIEKKKFLV PRDMSVGQFI
YILSARLHLS PGKALFVFVN NTLPQTAALM DSVYESYKDD DGFVYMCYSS EKTFG
