ATG8_ASPOR
ID ATG8_ASPOR Reviewed; 118 AA.
AC Q2UBH5;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Autophagy-related protein 8;
DE AltName: Full=Autophagy-related ubiquitin-like modifier atg8;
DE Flags: Precursor;
GN Name=atg8; ORFNames=AO090012000997;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16896216; DOI=10.1128/ec.00024-06;
RA Kikuma T., Ohneda M., Arioka M., Kitamoto K.;
RT "Functional analysis of the ATG8 homologue Aoatg8 and role of autophagy in
RT differentiation and germination in Aspergillus oryzae.";
RL Eukaryot. Cell 5:1328-1336(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC With atg4, mediates the delivery of the autophagosomes to the vacuole
CC via the microtubule cytoskeleton. Required for selective autophagic
CC degradation of the nucleus (nucleophagy) as well as for mitophagy which
CC contributes to regulate mitochondrial quantity and quality by
CC eliminating the mitochondria to a basal level to fulfill cellular
CC energy requirements and preventing excess ROS production. Participates
CC also in membrane fusion events that take place in the early secretory
CC pathway. Also involved in endoplasmic reticulum-specific autophagic
CC process and is essential for the survival of cells subjected to severe
CC ER stress. The atg8-PE conjugate mediates tethering between adjacent
CC membranes and stimulates membrane hemifusion, leading to expansion of
CC the autophagosomal membrane during autophagy (By similarity). Required
CC for both the differentiation of aerial hyphae and conidial germination
CC (PubMed:16896216). {ECO:0000250|UniProtKB:P38182,
CC ECO:0000269|PubMed:16896216}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000269|PubMed:16896216}; Lipid-anchor
CC {ECO:0000269|PubMed:16896216}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Lipid droplet
CC {ECO:0000269|PubMed:16896216}.
CC -!- PTM: The C-terminal 2 residues are removed by atg4 to expose Gly-116 at
CC the C-terminus. The c-terminal Gly is then amidated with
CC phosphatidylethanolamine by an activating system similar to that for
CC ubiquitin. {ECO:0000250|UniProtKB:P38182}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; AB246664; BAE93233.1; -; Genomic_DNA.
DR EMBL; AP007161; BAE61090.1; -; Genomic_DNA.
DR RefSeq; XP_001727929.1; XM_001727877.2.
DR AlphaFoldDB; Q2UBH5; -.
DR SMR; Q2UBH5; -.
DR STRING; 510516.Q2UBH5; -.
DR EnsemblFungi; BAE61090; BAE61090; AO090012000997.
DR GeneID; 5988403; -.
DR KEGG; aor:AO090012000997; -.
DR VEuPathDB; FungiDB:AO090012000997; -.
DR HOGENOM; CLU_119276_0_1_1; -.
DR OMA; AVYQEHK; -.
DR Proteomes; UP000006564; Chromosome 4.
DR GO; GO:0005776; C:autophagosome; IDA:AspGD.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:AspGD.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:AspGD.
DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell.
DR GO; GO:0000407; C:phagophore assembly site; IDA:AspGD.
DR GO; GO:0043936; P:asexual sporulation resulting in formation of a cellular spore; IMP:AspGD.
DR GO; GO:0006914; P:autophagy; IMP:AspGD.
DR GO; GO:0009306; P:protein secretion; IMP:AspGD.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasmic vesicle; Lipid droplet; Lipoprotein; Membrane;
KW Protein transport; Reference proteome; Transport; Ubl conjugation pathway;
KW Vacuole.
FT CHAIN 1..116
FT /note="Autophagy-related protein 8"
FT /id="PRO_0000317878"
FT PROPEP 117..118
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT /id="PRO_0000317879"
FT SITE 116..117
FT /note="Cleavage; by atg4"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT LIPID 116
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
SQ SEQUENCE 118 AA; 13748 MW; 8C78807255E14793 CRC64;
MRSKFKDEHP FEKRKAEAER IRQKYADRIP VICEKVEKSD IATIDKKKYL VPADLTVGQF
VYVIRKRIKL SPEKAIFIFV DEVLPPTAAL MSSIYEEHKD EDGFLYITYS GENTFGDL
