ATG8_BEAB2
ID ATG8_BEAB2 Reviewed; 118 AA.
AC J4UTT5;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2012, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Autophagy-related protein 8 {ECO:0000303|PubMed:27197558};
DE AltName: Full=Autophagy-related ubiquitin-like modifier ATG8 {ECO:0000250|UniProtKB:P38182};
DE Flags: Precursor;
GN Name=ATG8 {ECO:0000303|PubMed:27197558}; ORFNames=BBA_02302;
OS Beauveria bassiana (strain ARSEF 2860) (White muscardine disease fungus)
OS (Tritirachium shiotae).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Cordycipitaceae; Beauveria.
OX NCBI_TaxID=655819;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ARSEF 2860;
RX PubMed=22761991; DOI=10.1038/srep00483;
RA Xiao G., Ying S.-H., Zheng P., Wang Z.-L., Zhang S., Xie X.-Q., Shang Y.,
RA St Leger R.J., Zhao G.-P., Wang C., Feng M.-G.;
RT "Genomic perspectives on the evolution of fungal entomopathogenicity in
RT Beauveria bassiana.";
RL Sci. Rep. 2:483-483(2012).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RX PubMed=27197558; DOI=10.1038/srep26376;
RA Ying S.H., Liu J., Chu X.L., Xie X.Q., Feng M.G.;
RT "The autophagy-related genes BbATG1 and BbATG8 have different functions in
RT differentiation, stress resistance and virulence of mycopathogen Beauveria
RT bassiana.";
RL Sci. Rep. 6:26376-26376(2016).
RN [3]
RP INDUCTION.
RX PubMed=28557308; DOI=10.1111/1462-2920.13803;
RA Chu Z.J., Sun H.H., Zhu X.G., Ying S.H., Feng M.G.;
RT "Discovery of a new intravacuolar protein required for the autophagy,
RT development and virulence of Beauveria bassiana.";
RL Environ. Microbiol. 19:2806-2818(2017).
CC -!- FUNCTION: Ubiquitin-like modifier involved in cytoplasm to vacuole
CC transport (Cvt) vesicles and autophagosomes formation
CC (PubMed:27197558). With ATG4, mediates the delivery of the vesicles and
CC autophagosomes to the vacuole via the microtubule cytoskeleton (By
CC similarity). Required for selective autophagic degradation of the
CC nucleus (nucleophagy) as well as for mitophagy which contributes to
CC regulate mitochondrial quantity and quality by eliminating the
CC mitochondria to a basal level to fulfill cellular energy requirements
CC and preventing excess ROS production (By similarity). Participates also
CC in membrane fusion events that take place in the early secretory
CC pathway (By similarity). Also involved in endoplasmic reticulum-
CC specific autophagic process and is essential for the survival of cells
CC subjected to severe ER stress (By similarity). The ATG8-PE conjugate
CC mediates tethering between adjacent membranes and stimulates membrane
CC hemifusion, leading to expansion of the autophagosomal membrane during
CC autophagy (By similarity). Moreover not only conjugation, but also
CC subsequent ATG8-PE deconjugation is an important step required to
CC facilitate multiple events during macroautophagy, and especially for
CC efficient autophagosome biogenesis, the assembly of ATG9-containing
CC tubulovesicular clusters into phagophores/autophagosomes, and for the
CC disassembly of PAS-associated ATG components (By similarity).
CC Contributes to conidiation by regulating the conidial levels of the
CC conidiation-related protein CP15 and mediates fungal oxidation
CC resistance by controlling total superoxide dismutase (SOD) activity
CC (PubMed:27197558). {ECO:0000250|UniProtKB:P38182,
CC ECO:0000269|PubMed:27197558}.
CC -!- SUBUNIT: Conjugation to phosphatidylethanolamine (PE) leads to
CC homodimerization (By similarity). Interacts with ATG1, ATG3, ATG4, ATG7
CC and ATG12 (By similarity). {ECO:0000250|UniProtKB:P38182}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, cvt vesicle membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Cytoplasmic vesicle, autophagosome
CC membrane {ECO:0000269|PubMed:27197558}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC {ECO:0000269|PubMed:27197558}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Note=Membrane-associated through a
CC lipid anchor (By similarity). This association needs the 2 ubiquitin-
CC like systems required for cytoplasm to vacuole transport and autophagy
CC (By similarity). Localizes to both the isolation membrane (IM) and the
CC vacuole-isolation membrane contact site (VICS) during IM expansion (By
CC similarity). The IM is a membrane sac generated from the pre-
CC autophagosomal structure that ultimately expands to become a mature
CC autophagosome (By similarity). {ECO:0000250|UniProtKB:P38182}.
CC -!- PTM: The C-terminal Ser-117 and Ile-118 residues of ATG8 are removed by
CC ATG4 to expose Gly-116 at the C-terminus (By similarity). This Gly-116
CC forms then a thioester bond with ATG7 (E1-like activating enzyme)
CC before being transferred to ATG3 (the specific E2 conjugating enzyme),
CC in order to be finally amidated with phosphatidylethanolamine (By
CC similarity). This lipid modification anchors ATG8 to membranes and can
CC be reversed by ATG4, releasing soluble ATG8 (By similarity).
CC {ECO:0000250|UniProtKB:P38182}.
CC -!- DISRUPTION PHENOTYPE: Blocks autophagy (PubMed:27197558). Leads to
CC reduced conidial germination under starvation (PubMed:27197558).
CC Especially, leads to reduced CP15 levels in conidia (PubMed:27197558).
CC Exhibits also enhanced sensitivity to oxidative stress
CC (PubMed:27197558). Impairs growth of the mycelia on host cadavers and
CC considerably weakens virulence (PubMed:27197558).
CC {ECO:0000269|PubMed:27197558}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; JH725153; EJP69267.1; -; Genomic_DNA.
DR RefSeq; XP_008595621.1; XM_008597399.1.
DR AlphaFoldDB; J4UTT5; -.
DR SMR; J4UTT5; -.
DR STRING; 655819.J4UTT5; -.
DR EnsemblFungi; BB8028_0001g11640.1; BB8028_0001g11640.1; BB8028_0001g11640.
DR EnsemblFungi; EJP69267; EJP69267; BBA_02302.
DR GeneID; 19885314; -.
DR HOGENOM; CLU_119276_0_1_1; -.
DR InParanoid; J4UTT5; -.
DR Proteomes; UP000002762; Unassembled WGS sequence.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033110; C:Cvt vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Cytoplasmic vesicle; Lipoprotein; Membrane; Reference proteome;
KW Vacuole.
FT CHAIN 1..118
FT /note="Autophagy-related protein 8"
FT /id="PRO_0000443893"
FT PROPEP 117..118
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT /id="PRO_0000443894"
FT SITE 116..117
FT /note="Cleavage; by ATG4"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT LIPID 116
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
SQ SEQUENCE 118 AA; 13736 MW; 8B0FE8124D992793 CRC64;
MRSKFKDEHP FEKRKAEAER IRQKYADRIP VICEKVEKSD IATIDKKKYL VPSDLTVGQF
VYVIRKRIKL SPEKAIFIFV DEVLPPTAAL MSSIYEEHKD EDGFLYITYS GENTFGSI
