ATG8_COCH5
ID ATG8_COCH5 Reviewed; 119 AA.
AC M2SQA5;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=Autophagy-related protein 8 {ECO:0000303|PubMed:28800850};
DE AltName: Full=Autophagy-related ubiquitin-like modifier ATG8 {ECO:0000250|UniProtKB:P38182};
DE Flags: Precursor;
GN Name=ATG8 {ECO:0000303|PubMed:28800850}; ORFNames=COCHEDRAFT_1184566;
OS Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) (Southern
OS corn leaf blight fungus) (Bipolaris maydis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=701091;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O;
RX PubMed=23357949; DOI=10.1371/journal.pgen.1003233;
RA Condon B.J., Leng Y., Wu D., Bushley K.E., Ohm R.A., Otillar R., Martin J.,
RA Schackwitz W., Grimwood J., MohdZainudin N., Xue C., Wang R., Manning V.A.,
RA Dhillon B., Tu Z.J., Steffenson B.J., Salamov A., Sun H., Lowry S.,
RA LaButti K., Han J., Copeland A., Lindquist E., Barry K., Schmutz J.,
RA Baker S.E., Ciuffetti L.M., Grigoriev I.V., Zhong S., Turgeon B.G.;
RT "Comparative genome structure, secondary metabolite, and effector coding
RT capacity across Cochliobolus pathogens.";
RL PLoS Genet. 9:E1003233-E1003233(2013).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=28800850; DOI=10.1016/j.funbio.2017.05.008;
RA Sumita T., Izumitsu K., Tanaka C.;
RT "Characterization of the autophagy-related gene BmATG8 in Bipolaris
RT maydis.";
RL Fungal Biol. 121:785-797(2017).
CC -!- FUNCTION: Ubiquitin-like modifier involved in cytoplasm to vacuole
CC transport (Cvt) vesicles and autophagosomes formation
CC (PubMed:28800850). With ATG4, mediates the delivery of the vesicles and
CC autophagosomes to the vacuole via the microtubule cytoskeleton (By
CC similarity). Required for selective autophagic degradation of the
CC nucleus (nucleophagy) as well as for mitophagy which contributes to
CC regulate mitochondrial quantity and quality by eliminating the
CC mitochondria to a basal level to fulfill cellular energy requirements
CC and preventing excess ROS production (By similarity). Participates also
CC in membrane fusion events that take place in the early secretory
CC pathway (By similarity). Also involved in endoplasmic reticulum-
CC specific autophagic process and is essential for the survival of cells
CC subjected to severe ER stress (By similarity). The ATG8-PE conjugate
CC mediates tethering between adjacent membranes and stimulates membrane
CC hemifusion, leading to expansion of the autophagosomal membrane during
CC autophagy (By similarity). Moreover not only conjugation, but also
CC subsequent ATG8-PE deconjugation is an important step required to
CC facilitate multiple events during macroautophagy, and especially for
CC efficient autophagosome biogenesis, the assembly of ATG9-containing
CC tubulovesicular clusters into phagophores/autophagosomes, and for the
CC disassembly of PAS-associated ATG components (By similarity). Autophagy
CC is required for conidiation, aerial mycelial growth, and pseudothecia
CC formation, but not for host invasion (PubMed:28800850).
CC {ECO:0000250|UniProtKB:P38182, ECO:0000269|PubMed:28800850}.
CC -!- SUBUNIT: Conjugation to phosphatidylethanolamine (PE) leads to
CC homodimerization (By similarity). Interacts with ATG1, ATG3, ATG4, ATG7
CC and ATG12 (By similarity). {ECO:0000250|UniProtKB:P38182}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, cvt vesicle membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Cytoplasmic vesicle, autophagosome
CC membrane {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Note=Membrane-associated through a
CC lipid anchor (By similarity). This association needs the 2 ubiquitin-
CC like systems required for cytoplasm to vacuole transport and autophagy
CC (By similarity). Localizes to both the isolation membrane (IM) and the
CC vacuole-isolation membrane contact site (VICS) during IM expansion (By
CC similarity). The IM is a membrane sac generated from the pre-
CC autophagosomal structure that ultimately expands to become a mature
CC autophagosome (By similarity). {ECO:0000250|UniProtKB:P38182}.
CC -!- PTM: The C-terminal Glu-117, Ala-118 and Leu-119 residues of ATG8 are
CC removed by ATG4 to expose Gly-116 at the C-terminus (By similarity).
CC This Gly-116 forms then a thioester bond with ATG7 (E1-like activating
CC enzyme) before being transferred to ATG3 (the specific E2 conjugating
CC enzyme), in order to be finally amidated with phosphatidylethanolamine
CC (By similarity). This lipid modification anchors ATG8 to membranes and
CC can be reversed by ATG4, releasing soluble ATG8 (By similarity).
CC {ECO:0000250|UniProtKB:P38182}.
CC -!- DISRUPTION PHENOTYPE: Leads to deficient autophagy (PubMed:28800850).
CC Impairs conidial germination under starvation conditions, conidial
CC longevity, and ascospore maturation (PubMed:28800850).
CC {ECO:0000269|PubMed:28800850}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; KB445582; EMD87485.1; -; Genomic_DNA.
DR AlphaFoldDB; M2SQA5; -.
DR SMR; M2SQA5; -.
DR STRING; 701091.M2SQA5; -.
DR EnsemblFungi; EMD87485; EMD87485; COCHEDRAFT_1184566.
DR eggNOG; KOG1654; Eukaryota.
DR HOGENOM; CLU_119276_0_1_1; -.
DR OMA; AVYQEHK; -.
DR Proteomes; UP000016936; Unassembled WGS sequence.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033110; C:Cvt vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 3: Inferred from homology;
KW Autophagy; Cytoplasmic vesicle; Lipoprotein; Membrane; Protein transport;
KW Reference proteome; Transport; Vacuole.
FT CHAIN 1..119
FT /note="Autophagy-related protein 8"
FT /id="PRO_0000443889"
FT PROPEP 117..119
FT /note="Removed in mature form"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT /id="PRO_0000443890"
FT SITE 116..117
FT /note="Cleavage; by ATG4"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT LIPID 116
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
SQ SEQUENCE 119 AA; 13876 MW; 3F861030E25A1E48 CRC64;
MRSKFKDEHP FEKRKAEAER IRQKYNDRIP VICEKVEKSD IATIDKKKYL VPADLTVGQF
VYVIRKRIKL SPEKAIFIFV DEVLPPTAAL MSSIYEEHKD EDGFLYITYS GENTFGEAL
