ATG8_DICDI
ID ATG8_DICDI Reviewed; 122 AA.
AC Q86CR8; Q54LZ0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Autophagy-related protein 8;
DE AltName: Full=Autophagy-related ubiquitin-like modifier atg8;
DE Flags: Precursor;
GN Name=atg8; Synonyms=apg8; ORFNames=DDB_G0286191;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND FUNCTION.
RC STRAIN=AX3 / DH1;
RX PubMed=12626495; DOI=10.1074/jbc.m212467200;
RA Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT "Macroautophagy is required for multicellular development of the social
RT amoeba Dictyostelium discoideum.";
RL J. Biol. Chem. 278:17636-17645(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=14736886; DOI=10.1074/jbc.m311139200;
RA Otto G.P., Wu M.Y., Kazgan N., Anderson O.R., Kessin R.H.;
RT "Dictyostelium macroautophagy mutants vary in the severity of their
RT developmental defects.";
RL J. Biol. Chem. 279:15621-15629(2004).
RN [4]
RP INDUCTION.
RX PubMed=16469056; DOI=10.1111/j.1462-5822.2005.00633.x;
RA Farbrother P., Wagner C., Na J., Tunggal B., Morio T., Urushihara H.,
RA Tanaka Y., Schleicher M., Steinert M., Eichinger L.;
RT "Dictyostelium transcriptional host cell response upon infection with
RT Legionella.";
RL Cell. Microbiol. 8:438-456(2006).
CC -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC Participates in membrane fusion events that take place in the secretory
CC pathway (By similarity). {ECO:0000250, ECO:0000269|PubMed:12626495,
CC ECO:0000269|PubMed:14736886}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000250}; Lipid-anchor {ECO:0000250}.
CC -!- INDUCTION: By Legionella pneumophila infection.
CC {ECO:0000269|PubMed:16469056}.
CC -!- PTM: The C-terminal 3 residues of atg8 are removed by atg4 to expose
CC Gly-119 at the C-terminus. This Gly-119 forms then a thioester bond
CC with the 'Cys-563' of atg7 (E1-like activating enzyme) before being
CC transferred to the 'Cys-288' of atg3 (the specific E2 conjugating
CC enzyme), in order to be finally amidated with phosphatidylethanolamine.
CC This lipid modification anchors atg8 to membranes and can be reversed
CC by atg4, releasing soluble atg8 (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; AY191015; AAO39078.1; -; Genomic_DNA.
DR EMBL; AAFI02000085; EAL64271.1; -; Genomic_DNA.
DR RefSeq; XP_637841.1; XM_632749.1.
DR AlphaFoldDB; Q86CR8; -.
DR SMR; Q86CR8; -.
DR STRING; 44689.DDB0191413; -.
DR PaxDb; Q86CR8; -.
DR EnsemblProtists; EAL64271; EAL64271; DDB_G0286191.
DR GeneID; 8625555; -.
DR KEGG; ddi:DDB_G0286191; -.
DR dictyBase; DDB_G0286191; atg8a.
DR eggNOG; KOG1654; Eukaryota.
DR HOGENOM; CLU_119276_0_1_1; -.
DR InParanoid; Q86CR8; -.
DR OMA; AVYQEHK; -.
DR PhylomeDB; Q86CR8; -.
DR Reactome; R-DDI-1632852; Macroautophagy.
DR Reactome; R-DDI-8854214; TBC/RABGAPs.
DR Reactome; R-DDI-8934903; Receptor Mediated Mitophagy.
DR Reactome; R-DDI-9664873; Pexophagy.
DR Reactome; R-DDI-9755511; KEAP1-NFE2L2 pathway.
DR PRO; PR:Q86CR8; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005776; C:autophagosome; IDA:dictyBase.
DR GO; GO:0000421; C:autophagosome membrane; IDA:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0140220; C:pathogen-containing vacuole; IDA:dictyBase.
DR GO; GO:0097352; P:autophagosome maturation; IMP:dictyBase.
DR GO; GO:1905037; P:autophagosome organization; IMP:dictyBase.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:dictyBase.
DR GO; GO:0006995; P:cellular response to nitrogen starvation; IMP:dictyBase.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:dictyBase.
DR GO; GO:0016236; P:macroautophagy; IMP:dictyBase.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR GO; GO:1901355; P:response to rapamycin; IMP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 2: Evidence at transcript level;
KW Autophagy; Cytoplasmic vesicle; Lipoprotein; Membrane; Protein transport;
KW Reference proteome; Transport; Ubl conjugation pathway.
FT CHAIN 1..119
FT /note="Autophagy-related protein 8"
FT /id="PRO_0000327833"
FT PROPEP 120..122
FT /note="Removed in mature form"
FT /id="PRO_0000327834"
FT SITE 119..120
FT /note="Cleavage; by ATG4"
FT LIPID 119
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 122 AA; 14097 MW; 3BB7B5EA4BE51CBF CRC64;
MVHVSSFKND HPLDKRREVA ERIRSKYLDR IPVIVEKAPR SDAPDIDKKK YLVPADITVG
KFVYEIRKHM TKVSAEKAIY LFVNNTIPPT AALISQIYER YKDEDGFLYI TYSGENTFGS
DL
