ATG8_PICPA
ID ATG8_PICPA Reviewed; 125 AA.
AC Q8NJJ4;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Autophagy-related protein 8;
DE AltName: Full=Autophagy-related ubiquitin-like modifier ATG8;
DE AltName: Full=Pexophagy zeocin-resistant mutant protein 2;
DE Flags: Precursor;
GN Name=ATG8; Synonyms=PAZ2;
OS Komagataella pastoris (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=4922;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11856375; DOI=10.1046/j.1356-9597.2001.00499.x;
RA Mukaiyama H., Oku M., Baba M., Samizo T., Hammond A.T., Glick B.S.,
RA Kato N., Sakai Y.;
RT "Paz2 and 13 other PAZ gene products regulate vacuolar engulfment of
RT peroxisomes during micropexophagy.";
RL Genes Cells 7:75-90(2002).
RN [2]
RP NOMENCLATURE.
RX PubMed=14536056; DOI=10.1016/s1534-5807(03)00296-x;
RA Klionsky D.J., Cregg J.M., Dunn W.A. Jr., Emr S.D., Sakai Y.,
RA Sandoval I.V., Sibirny A., Subramani S., Thumm M., Veenhuis M., Ohsumi Y.;
RT "A unified nomenclature for yeast autophagy-related genes.";
RL Dev. Cell 5:539-545(2003).
RN [3]
RP FUNCTION, CLEAVAGE, AND MUTAGENESIS OF GLY-116.
RX PubMed=13679515; DOI=10.1091/mbc.e03-05-0340;
RA Mukaiyama H., Baba M., Osumi M., Aoyagi S., Kato N., Ohsumi Y., Sakai Y.;
RT "Modification of a ubiquitin-like protein Paz2 conducted micropexophagy
RT through formation of a novel membrane structure.";
RL Mol. Biol. Cell 15:58-70(2004).
RN [4]
RP INTERACTION WITH ATG30 AND ATG32.
RX PubMed=23559066; DOI=10.1038/embor.2013.40;
RA Farre J.C., Burkenroad A., Burnett S.F., Subramani S.;
RT "Phosphorylation of mitophagy and pexophagy receptors coordinates their
RT interaction with Atg8 and Atg11.";
RL EMBO Rep. 14:441-449(2013).
CC -!- FUNCTION: Ubiquitin-like modifier involved in autophagosomes formation.
CC With ATG4, mediates the delivery of the autophagosomes to the vacuole
CC via the microtubule cytoskeleton. Required for selective autophagic
CC degradation of the nucleus (nucleophagy) as well as for mitophagy which
CC contributes to regulate mitochondrial quantity and quality by
CC eliminating the mitochondria to a basal level to fulfill cellular
CC energy requirements and preventing excess ROS production. Participates
CC also in membrane fusion events that take place in the early secretory
CC pathway. Also involved in endoplasmic reticulum-specific autophagic
CC process and is essential for the survival of cells subjected to severe
CC ER stress. The ATG8-PE conjugate mediates tethering between adjacent
CC membranes and stimulates membrane hemifusion, leading to expansion of
CC the autophagosomal membrane during autophagy.
CC {ECO:0000250|UniProtKB:P38182, ECO:0000269|PubMed:13679515}.
CC -!- SUBUNIT: Interacts with ATG4 (By similarity). Interacts with ATG30 and
CC ATG32 (PubMed:23559066). {ECO:0000250, ECO:0000269|PubMed:23559066}.
CC -!- INTERACTION:
CC Q8NJJ4; I6LAD1: ATG30; NbExp=3; IntAct=EBI-8849485, EBI-8849497;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, autophagosome membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}. Vacuole membrane
CC {ECO:0000250|UniProtKB:P38182}; Lipid-anchor
CC {ECO:0000250|UniProtKB:P38182}.
CC -!- PTM: The last C-terminal 9 residues are removed to expose Gly-116 at
CC the C-terminus. The C-terminal Gly is then amidated with
CC phosphatidylethanolamine by an activating system similar to that for
CC ubiquitine. {ECO:0000250|UniProtKB:P38182}.
CC -!- SIMILARITY: Belongs to the ATG8 family. {ECO:0000305}.
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DR EMBL; AY058219; AAL25848.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8NJJ4; -.
DR SMR; Q8NJJ4; -.
DR IntAct; Q8NJJ4; 1.
DR MINT; Q8NJJ4; -.
DR GO; GO:0000421; C:autophagosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR004241; Atg8-like.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR10969; PTHR10969; 1.
DR Pfam; PF02991; ATG8; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasmic vesicle; Lipoprotein; Membrane; Protein transport;
KW Transport; Ubl conjugation pathway; Vacuole.
FT CHAIN 1..116
FT /note="Autophagy-related protein 8"
FT /id="PRO_0000017232"
FT PROPEP 117..125
FT /note="Removed in mature form"
FT /evidence="ECO:0000305|PubMed:23559066"
FT /id="PRO_0000017233"
FT SITE 116..117
FT /note="Cleavage; by ATG4"
FT /evidence="ECO:0000305|PubMed:23559066"
FT LIPID 116
FT /note="Phosphatidylethanolamine amidated glycine"
FT /evidence="ECO:0000250|UniProtKB:P38182"
FT MUTAGEN 116
FT /note="G->A: No cleaving of the nine last C-terminal
FT residues by ATG4. Defect in micropexophagy."
FT /evidence="ECO:0000269|PubMed:13679515"
SQ SEQUENCE 125 AA; 14662 MW; 72C8A87531D4DB0C CRC64;
MRSQFKDEHP FEKRKAEAMR IRYKFADRIP VICEKVEKSD IPEVDKRKYL VPCDLTVGQF
VYVIRKRIKI PSEKAIFIFI DDILPPTAAL MSKVYEEHKD EDGFLYVLYS GENTFGDIPG
VEEIE
