PSBE_HORVU
ID PSBE_HORVU Reviewed; 83 AA.
AC P69390; A1E9K7; P05169; P10879; Q95H57;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Cytochrome b559 subunit alpha {ECO:0000255|HAMAP-Rule:MF_00642};
DE AltName: Full=PSII reaction center subunit V {ECO:0000255|HAMAP-Rule:MF_00642};
GN Name=psbE {ECO:0000255|HAMAP-Rule:MF_00642};
OS Hordeum vulgare (Barley).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Sabarlis;
RX PubMed=2654886; DOI=10.1093/nar/17.7.2858;
RA Chakhmakhcheva O.G., Andreeva A.V., Buryakova A.A., Reverdatto S.V.,
RA Efimov V.A.;
RT "Nucleotide sequence of the barley chloroplast psbE, psbF genes and
RT flanking regions.";
RL Nucleic Acids Res. 17:2858-2858(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Sabarlis;
RX PubMed=1804121;
RA Efimov V.A., Andreeva A.V., Reverdatto S.V., Chakhmakhcheva O.G.;
RT "Photosystem II of rye. Nucleotide sequence of the psbB, psbC, psbE, psbF,
RT psbH genes of rye and chloroplast DNA regions adjacent to them.";
RL Bioorg. Khim. 17:1369-1385(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3256307; DOI=10.1007/bf02908413;
RA Krupinska K., Berry-Lowe S.;
RT "Characterization and in vitro expression of the cytochrome b-559 genes of
RT barley. I. Localization and sequence of the genes.";
RL Carlsberg Res. Commun. 53:43-55(1988).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3255312; DOI=10.1007/bf02907180;
RA Krupinska K.;
RT "Characterization and in vitro expression of the cytochrome b-559 genes of
RT barley. II. In vitro transcription and translation.";
RL Carlsberg Res. Commun. 53:233-246(1988).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Morex;
RX PubMed=17534593; DOI=10.1007/s00122-007-0567-4;
RA Saski C., Lee S.-B., Fjellheim S., Guda C., Jansen R.K., Luo H.,
RA Tomkins J., Rognli O.A., Daniell H., Clarke J.L.;
RT "Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor
RT and Agrostis stolonifera, and comparative analyses with other grass
RT genomes.";
RL Theor. Appl. Genet. 115:571-590(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Steffi;
RX PubMed=19137553; DOI=10.1002/pmic.200800337;
RA Ploescher M., Granvogl B., Zoryan M., Reisinger V., Eichacker L.A.;
RT "Mass spectrometric characterization of membrane integral low molecular
RT weight proteins from photosystem II in barley etioplasts.";
RL Proteomics 9:625-635(2009).
CC -!- FUNCTION: This b-type cytochrome is tightly associated with the
CC reaction center of photosystem II (PSII). PSII is a light-driven
CC water:plastoquinone oxidoreductase that uses light energy to abstract
CC electrons from H(2)O, generating O(2) and a proton gradient
CC subsequently used for ATP formation. It consists of a core antenna
CC complex that captures photons, and an electron transfer chain that
CC converts photonic excitation into a charge separation.
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00642};
CC Note=With its partner (PsbF) binds heme. PSII binds additional
CC chlorophylls, carotenoids and specific lipids. {ECO:0000255|HAMAP-
CC Rule:MF_00642};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit. PSII is
CC composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD,
CC PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ,
CC Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes (By
CC similarity). Detected in both etioplasts and green leaves; PSII is only
CC assembled in green leaves (PubMed:19137553). {ECO:0000255|HAMAP-
CC Rule:MF_00642, ECO:0000269|PubMed:19137553}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00642, ECO:0000305|PubMed:19137553}; Single-
CC pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00642,
CC ECO:0000305|PubMed:19137553}.
CC -!- SIMILARITY: Belongs to the PsbE/PsbF family. {ECO:0000255|HAMAP-
CC Rule:MF_00642}.
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DR EMBL; X14108; CAA32270.1; -; Genomic_DNA.
DR EMBL; M35977; AAA84044.1; -; Genomic_DNA.
DR EMBL; M35616; AAA84048.1; -; Genomic_DNA.
DR EMBL; EF115541; ABK79429.1; -; Genomic_DNA.
DR PIR; A29956; A29956.
DR RefSeq; YP_874669.1; NC_008590.1.
DR AlphaFoldDB; P69390; -.
DR SMR; P69390; -.
DR GeneID; 4525055; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR Gene3D; 1.20.5.860; -; 1.
DR HAMAP; MF_00642; PSII_PsbE; 1.
DR InterPro; IPR006217; PSII_cyt_b559_asu.
DR InterPro; IPR037025; PSII_cyt_b559_asu_sf.
DR InterPro; IPR006216; PSII_cyt_b559_CS.
DR InterPro; IPR013081; PSII_cyt_b559_N.
DR InterPro; IPR013082; PSII_cytb559_asu_lum.
DR Pfam; PF00283; Cytochrom_B559; 1.
DR Pfam; PF00284; Cytochrom_B559a; 1.
DR PIRSF; PIRSF000036; PsbE; 1.
DR TIGRFAMs; TIGR01332; cyt_b559_alpha; 1.
DR PROSITE; PS00537; CYTOCHROME_B559; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Photosynthesis; Photosystem II; Plastid; Thylakoid; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..83
FT /note="Cytochrome b559 subunit alpha"
FT /id="PRO_0000200313"
FT TRANSMEM 21..35
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT BINDING 23
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_note="ligand shared with beta subunit"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT CONFLICT 16
FT /note="S -> C (in Ref. 1; CAA32270)"
FT /evidence="ECO:0000305"
FT CONFLICT 23
FT /note="H -> R (in Ref. 1; CAA32270)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 83 AA; 9445 MW; 4C73918BCABACDDD CRC64;
MSGSTGERSF ADIITSIRYW VIHSITIPSL FIAGWLFVST GLAYDVFGSP RPNEYFTESR
QGIPLITDRF DSLEQLDEFS RSF
