PSBE_PEA
ID PSBE_PEA Reviewed; 83 AA.
AC P13554;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Cytochrome b559 subunit alpha {ECO:0000255|HAMAP-Rule:MF_00642};
DE AltName: Full=PSII reaction center subunit V {ECO:0000255|HAMAP-Rule:MF_00642};
GN Name=psbE {ECO:0000255|HAMAP-Rule:MF_00642};
OS Pisum sativum (Garden pea).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2766383; DOI=10.1007/bf00435508;
RA Willey D.L., Gray J.C.;
RT "Two small open reading frames are co-transcribed with the pea chloroplast
RT genes for the polypeptides of cytochrome b-559.";
RL Curr. Genet. 15:213-220(1989).
RN [2]
RP MASS SPECTROMETRY.
RX PubMed=9407102; DOI=10.1074/jbc.272.52.33153;
RA Sharma J., Panico M., Barber J., Morris H.R.;
RT "Purification and determination of intact molecular mass by electrospray
RT ionization mass spectrometry of the photosystem II reaction center
RT subunits.";
RL J. Biol. Chem. 272:33153-33157(1997).
CC -!- FUNCTION: This b-type cytochrome is tightly associated with the
CC reaction center of photosystem II (PSII). PSII is a light-driven
CC water:plastoquinone oxidoreductase that uses light energy to abstract
CC electrons from H(2)O, generating O(2) and a proton gradient
CC subsequently used for ATP formation. It consists of a core antenna
CC complex that captures photons, and an electron transfer chain that
CC converts photonic excitation into a charge separation.
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00642};
CC Note=With its partner (PsbF) binds heme. PSII binds additional
CC chlorophylls, carotenoids and specific lipids. {ECO:0000255|HAMAP-
CC Rule:MF_00642};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit. PSII is
CC composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD,
CC PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ,
CC Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes.
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00642}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- MASS SPECTROMETRY: Mass=9283.7; Mass_error=0.8; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:9407102};
CC -!- SIMILARITY: Belongs to the PsbE/PsbF family. {ECO:0000255|HAMAP-
CC Rule:MF_00642}.
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DR EMBL; X15767; CAA33772.1; -; Genomic_DNA.
DR PIR; A48310; A48310.
DR RefSeq; YP_003587551.1; NC_014057.1.
DR PDB; 5XNL; EM; 2.70 A; E/e=1-83.
DR PDB; 5XNM; EM; 3.20 A; E/e=1-83.
DR PDB; 6YP7; EM; 3.80 A; E/e=8-82.
DR PDBsum; 5XNL; -.
DR PDBsum; 5XNM; -.
DR PDBsum; 6YP7; -.
DR AlphaFoldDB; P13554; -.
DR SMR; P13554; -.
DR EnsemblPlants; Psat0s6989g0160.1; Psat0s6989g0160.1.cds1; Psat0s6989g0160.
DR GeneID; 9073099; -.
DR Gramene; Psat0s6989g0160.1; Psat0s6989g0160.1.cds1; Psat0s6989g0160.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR Gene3D; 1.20.5.860; -; 1.
DR HAMAP; MF_00642; PSII_PsbE; 1.
DR InterPro; IPR006217; PSII_cyt_b559_asu.
DR InterPro; IPR037025; PSII_cyt_b559_asu_sf.
DR InterPro; IPR006216; PSII_cyt_b559_CS.
DR InterPro; IPR013081; PSII_cyt_b559_N.
DR InterPro; IPR013082; PSII_cytb559_asu_lum.
DR Pfam; PF00283; Cytochrom_B559; 1.
DR Pfam; PF00284; Cytochrom_B559a; 1.
DR PIRSF; PIRSF000036; PsbE; 1.
DR TIGRFAMs; TIGR01332; cyt_b559_alpha; 1.
DR PROSITE; PS00537; CYTOCHROME_B559; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Electron transport; Heme; Iron; Membrane;
KW Metal-binding; Photosynthesis; Photosystem II; Plastid; Thylakoid;
KW Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9407102"
FT CHAIN 2..83
FT /note="Cytochrome b559 subunit alpha"
FT /id="PRO_0000200329"
FT TRANSMEM 21..35
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT BINDING 23
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_note="ligand shared with beta subunit"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT HELIX 10..14
FT /evidence="ECO:0007829|PDB:5XNL"
FT HELIX 17..39
FT /evidence="ECO:0007829|PDB:5XNL"
FT HELIX 42..46
FT /evidence="ECO:0007829|PDB:5XNL"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:5XNM"
FT STRAND 69..71
FT /evidence="ECO:0007829|PDB:5XNM"
FT HELIX 72..81
FT /evidence="ECO:0007829|PDB:5XNL"
SQ SEQUENCE 83 AA; 9415 MW; F681641BCABAD2C1 CRC64;
MSGSTGERSF ADIITSIRYW IIHSITIPSL FIAGWLFVST GLAYDVFGSP RPNEYFTETR
QGIPLITGRF DSLEQLDEFS RSF
