ID   AACC3_ENTCL             Reviewed;         286 AA.
AC   P0A256; P13245;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Aminoglycoside N(3)-acetyltransferase III;
DE            EC=2.3.1.81;
DE   AltName: Full=ACC(3)-III;
DE   AltName: Full=Aminocyclitol 3-N-acetyltransferase type III;
DE   AltName: Full=Gentamicin-(3)-N-acetyl-transferase;
GN   Name=aacC3; Synonyms=aac3-VA, aacC2;
OS   Enterobacter cloacae.
OG   Plasmid pJV03.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
OX   NCBI_TaxID=550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2552900; DOI=10.1128/aac.33.8.1153;
RA   Vliegenthart J.S., Ketelaar-Van Gaalen P.A.G., van de Klundert J.A.M.;
RT   "Nucleotide sequence of the aacC2 gene, a gentamicin resistance determinant
RT   involved in a hospital epidemic of multiply resistant members of the family
RT   Enterobacteriaceae.";
RL   Antimicrob. Agents Chemother. 33:1153-1159(1989).
CC   -!- FUNCTION: Resistance to antibiotics containing the 2-deoxy-streptamine
CC       ring including gentamicin, kanamycin, tobramycin, neomycin and
CC       apramycin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2-deoxystreptamine antibiotic + acetyl-CoA = an N(3')-
CC         acetyl-2-deoxystreptamine antibiotic + CoA + H(+);
CC         Xref=Rhea:RHEA:12665, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57921, ChEBI:CHEBI:77452; EC=2.3.1.81;
CC   -!- SIMILARITY: Belongs to the antibiotic N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X51534; CAA35913.1; -; Genomic_DNA.
DR   PIR; S09651; S09651.
DR   RefSeq; WP_000557452.1; NZ_QWFQ01000038.1.
DR   AlphaFoldDB; P0A256; -.
DR   SMR; P0A256; -.
DR   KEGG; ag:CAA35913; -.
DR   GO; GO:0046353; F:aminoglycoside 3-N-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003679; Amioglycoside_AcTrfase.
DR   InterPro; IPR028345; Antibiotic_NAT-like.
DR   PANTHER; PTHR11104; PTHR11104; 1.
DR   Pfam; PF02522; Antibiotic_NAT; 1.
DR   SUPFAM; SSF110710; SSF110710; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Plasmid; Transferase.
FT   CHAIN           1..286
FT                   /note="Aminoglycoside N(3)-acetyltransferase III"
FT                   /id="PRO_0000068545"
SQ   SEQUENCE   286 AA;  30609 MW;  AE30E9D093CBBD1F CRC64;
     MHTRKAITEA IRKLGVQTGD LLMVHASLKA IGPVEGGAET VVAALRSAVG PTGTVMGYAS
     WDRSPYEETL NGARLDDKAR RTWPPFDPAT AGTYRGFGLL NQFLVQAPGA RRSAHPDASM
     VAVGPLAETL TEPHELGHAL GEGSPVERFV RLGGKALLLG APLNSVTALH YAEAVADIPN
     KRWVTYEMPM LGRNGEVAWK TASEYDSNGI LDCFAIEGKP DAVETIANAY VKLGRHREGV
     VGFAQCYLFD AQDIVTFGVT YLEKHFGATP IVPAHEAAQR SCEPSG