PSBE_WHEAT
ID PSBE_WHEAT Reviewed; 83 AA.
AC P69386; P05169; P10879; Q95H57;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=Cytochrome b559 subunit alpha {ECO:0000255|HAMAP-Rule:MF_00642};
DE AltName: Full=PSII reaction center subunit V {ECO:0000255|HAMAP-Rule:MF_00642};
GN Name=psbE {ECO:0000255|HAMAP-Rule:MF_00642};
OS Triticum aestivum (Wheat).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Hird S.M., Willey D.L., Dyer T.A., Gray J.C.;
RT "Location and nucleotide sequence of the gene for cytochrome b-559 in wheat
RT chloroplast DNA.";
RL Mol. Gen. Genet. 203:95-100(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-16.
RC STRAIN=cv. Sentry; TISSUE=Leaf;
RA Webber A.N., Hird S.M., Packman L.C., Dyer T.A., Gray J.C.;
RT "A photosystem II polypeptide is encoded by an open reading frame co-
RT transcribed with genes for cytochrome b-559 in wheat chloroplast DNA.";
RL Plant Mol. Biol. 12:141-151(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring;
RA Ogihara Y., Isono K., Kojima T., Endo A., Hanaoka M., Shiina T.,
RA Terachi T., Utsugi S., Murata M., Mori N., Takumi S., Ikeo K., Gojobori T.,
RA Murai R., Murai K., Matsuoka Y., Ohnishi Y., Tajiri H., Tsunewaki K.;
RT "Chinese spring wheat (Triticum aestivum L.) chloroplast genome: complete
RT sequence and contig clones.";
RL Plant Mol. Biol. Rep. 18:243-253(2000).
RN [4]
RP PROTEIN SEQUENCE OF 2-15.
RX PubMed=2644131; DOI=10.1016/0014-5793(89)80482-x;
RA Ikeuchi M., Takio K., Inoue Y.;
RT "N-terminal sequencing of photosystem II low-molecular-mass proteins. 5 and
RT 4.1 kDa components of the O2-evolving core complex from higher plants.";
RL FEBS Lett. 242:263-269(1989).
CC -!- FUNCTION: This b-type cytochrome is tightly associated with the
CC reaction center of photosystem II (PSII). PSII is a light-driven
CC water:plastoquinone oxidoreductase that uses light energy to abstract
CC electrons from H(2)O, generating O(2) and a proton gradient
CC subsequently used for ATP formation. It consists of a core antenna
CC complex that captures photons, and an electron transfer chain that
CC converts photonic excitation into a charge separation.
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00642};
CC Note=With its partner (PsbF) binds heme. PSII binds additional
CC chlorophylls, carotenoids and specific lipids. {ECO:0000255|HAMAP-
CC Rule:MF_00642};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit. PSII is
CC composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD,
CC PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ,
CC Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes.
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00642}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00642}.
CC -!- SIMILARITY: Belongs to the PsbE/PsbF family. {ECO:0000255|HAMAP-
CC Rule:MF_00642}.
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DR EMBL; X03776; CAA27405.1; -; Genomic_DNA.
DR EMBL; X15225; CAA33294.1; -; Genomic_DNA.
DR EMBL; AB042240; BAB47050.1; -; Genomic_DNA.
DR PIR; S03621; CBWT5E.
DR RefSeq; NP_114275.1; NC_002762.1.
DR AlphaFoldDB; P69386; -.
DR SMR; P69386; -.
DR STRING; 4565.EPlTAEP00000010041; -.
DR PRIDE; P69386; -.
DR EnsemblPlants; TraesCAD_scaffold_023365_01G000100.1; TraesCAD_scaffold_023365_01G000100.1; TraesCAD_scaffold_023365_01G000100.
DR EnsemblPlants; TraesCS1A02G148500.1; TraesCS1A02G148500.1.cds1; TraesCS1A02G148500.
DR EnsemblPlants; TraesCS1D02G295400.1; TraesCS1D02G295400.1.cds1; TraesCS1D02G295400.
DR EnsemblPlants; TraesCS2D02G270700.1; TraesCS2D02G270700.1.cds1; TraesCS2D02G270700.
DR EnsemblPlants; TraesCS2D02G553800.1; TraesCS2D02G553800.1.cds1; TraesCS2D02G553800.
DR EnsemblPlants; TraesCSU02G261900.1; TraesCSU02G261900.1.cds1; TraesCSU02G261900.
DR EnsemblPlants; TraesWEE_scaffold_058266_01G000100.1; TraesWEE_scaffold_058266_01G000100.1; TraesWEE_scaffold_058266_01G000100.
DR EnsemblPlants; TraesWEE_scaffold_219805_01G000500.1; TraesWEE_scaffold_219805_01G000500.1; TraesWEE_scaffold_219805_01G000500.
DR GeneID; 803176; -.
DR Gramene; TraesCAD_scaffold_023365_01G000100.1; TraesCAD_scaffold_023365_01G000100.1; TraesCAD_scaffold_023365_01G000100.
DR Gramene; TraesCS1A02G148500.1; TraesCS1A02G148500.1.cds1; TraesCS1A02G148500.
DR Gramene; TraesCS1D02G295400.1; TraesCS1D02G295400.1.cds1; TraesCS1D02G295400.
DR Gramene; TraesCS2D02G270700.1; TraesCS2D02G270700.1.cds1; TraesCS2D02G270700.
DR Gramene; TraesCS2D02G553800.1; TraesCS2D02G553800.1.cds1; TraesCS2D02G553800.
DR Gramene; TraesCSU02G261900.1; TraesCSU02G261900.1.cds1; TraesCSU02G261900.
DR Gramene; TraesWEE_scaffold_058266_01G000100.1; TraesWEE_scaffold_058266_01G000100.1; TraesWEE_scaffold_058266_01G000100.
DR Gramene; TraesWEE_scaffold_219805_01G000500.1; TraesWEE_scaffold_219805_01G000500.1; TraesWEE_scaffold_219805_01G000500.
DR KEGG; taes:803176; -.
DR eggNOG; ENOG502S2SP; Eukaryota.
DR HOGENOM; CLU_194095_0_0_1; -.
DR OMA; VRYWVIH; -.
DR Proteomes; UP000019116; Chloroplast.
DR ExpressionAtlas; P69386; baseline.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR Gene3D; 1.20.5.860; -; 1.
DR HAMAP; MF_00642; PSII_PsbE; 1.
DR InterPro; IPR006217; PSII_cyt_b559_asu.
DR InterPro; IPR037025; PSII_cyt_b559_asu_sf.
DR InterPro; IPR006216; PSII_cyt_b559_CS.
DR InterPro; IPR013081; PSII_cyt_b559_N.
DR InterPro; IPR013082; PSII_cytb559_asu_lum.
DR Pfam; PF00283; Cytochrom_B559; 1.
DR Pfam; PF00284; Cytochrom_B559a; 1.
DR PIRSF; PIRSF000036; PsbE; 1.
DR TIGRFAMs; TIGR01332; cyt_b559_alpha; 1.
DR PROSITE; PS00537; CYTOCHROME_B559; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Electron transport; Heme; Iron;
KW Membrane; Metal-binding; Photosynthesis; Photosystem II; Plastid;
KW Reference proteome; Thylakoid; Transmembrane; Transmembrane helix;
KW Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:2644131, ECO:0000269|Ref.2"
FT CHAIN 2..83
FT /note="Cytochrome b559 subunit alpha"
FT /id="PRO_0000200341"
FT TRANSMEM 21..35
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT BINDING 23
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_note="ligand shared with beta subunit"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00642"
FT CONFLICT 50
FT /note="P -> L (in Ref. 3; BAB47050)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 83 AA; 9445 MW; 4C73918BCABACDDD CRC64;
MSGSTGERSF ADIITSIRYW VIHSITIPSL FIAGWLFVST GLAYDVFGSP RPNEYFTESR
QGIPLITDRF DSLEQLDEFS RSF
