PSBF_CHLRE
ID PSBF_CHLRE Reviewed; 44 AA.
AC Q08363; B7U1I3;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Cytochrome b559 subunit beta {ECO:0000255|HAMAP-Rule:MF_00643};
DE AltName: Full=PSII reaction center subunit VI {ECO:0000255|HAMAP-Rule:MF_00643};
GN Name=psbF {ECO:0000255|HAMAP-Rule:MF_00643};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1617741; DOI=10.1007/bf00351664;
RA Fong S.E., Surzycki S.J.;
RT "Organization and structure of plastome psbF, psbL, petG and ORF712 genes
RT in Chlamydomonas reinhardtii.";
RL Curr. Genet. 21:527-530(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=137c / CC-125;
RX PubMed=7803458; DOI=10.1016/0005-2728(94)90067-1;
RA Alizadeh S., Nechustai R., Barber J., Nixon P.;
RT "Nucleotide sequence of the psbE, psbF and trnM genes from the chloroplast
RT genome of Chlamydomonas reinhardtii.";
RL Biochim. Biophys. Acta 1188:439-442(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=137c / CC-125;
RX PubMed=7700232; DOI=10.1007/bf00298966;
RA Mor T.S., Ohad I., Hirschberg J., Pakrasi H.B.;
RT "An unusual organization of the genes encoding cytochrome b559 in
RT Chlamydomonas reinhardtii: psbE and psbF genes are separately transcribed
RT from different regions of the plastid chromosome.";
RL Mol. Gen. Genet. 246:600-604(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [5]
RP IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
CC -!- FUNCTION: This b-type cytochrome is tightly associated with the
CC reaction center of photosystem II (PSII). PSII is a light-driven
CC water:plastoquinone oxidoreductase that uses light energy to abstract
CC electrons from H(2)O, generating O(2) and a proton gradient
CC subsequently used for ATP formation. It consists of a core antenna
CC complex that captures photons, and an electron transfer chain that
CC converts photonic excitation into a charge separation.
CC {ECO:0000255|HAMAP-Rule:MF_00643}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00643};
CC Note=With its partner (PsbE) binds heme. PSII binds additional
CC chlorophylls, carotenoids and specific lipids. {ECO:0000255|HAMAP-
CC Rule:MF_00643};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit. PSII is
CC composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD,
CC PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ,
CC Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes.
CC {ECO:0000255|HAMAP-Rule:MF_00643}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00643}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00643}.
CC -!- SIMILARITY: Belongs to the PsbE/PsbF family. {ECO:0000255|HAMAP-
CC Rule:MF_00643}.
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DR EMBL; X66250; CAA46977.1; -; Genomic_DNA.
DR EMBL; X80195; CAA56486.1; -; Genomic_DNA.
DR EMBL; X79564; CAA56101.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50130.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00944.1; -; Genomic_DNA.
DR PIR; S53883; S53883.
DR RefSeq; NP_958399.1; NC_005353.1.
DR PDB; 6KAC; EM; 2.70 A; F/f=1-44.
DR PDB; 6KAD; EM; 3.40 A; F/f=1-44.
DR PDB; 6KAF; EM; 3.73 A; F/f=1-44.
DR PDBsum; 6KAC; -.
DR PDBsum; 6KAD; -.
DR PDBsum; 6KAF; -.
DR AlphaFoldDB; Q08363; -.
DR SMR; Q08363; -.
DR STRING; 3055.DAA00944; -.
DR PaxDb; Q08363; -.
DR PRIDE; Q08363; -.
DR GeneID; 2716994; -.
DR KEGG; cre:ChreCp043; -.
DR eggNOG; ENOG502SEUE; Eukaryota.
DR HOGENOM; CLU_211753_1_0_1; -.
DR InParanoid; Q08363; -.
DR OrthoDB; 1637060at2759; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR HAMAP; MF_00643; PSII_PsbF; 1.
DR InterPro; IPR006241; PSII_cyt_b559_bsu.
DR InterPro; IPR006216; PSII_cyt_b559_CS.
DR InterPro; IPR013081; PSII_cyt_b559_N.
DR PANTHER; PTHR33391:SF14; PTHR33391:SF14; 1.
DR Pfam; PF00283; Cytochrom_B559; 1.
DR PIRSF; PIRSF000037; PsbF; 1.
DR TIGRFAMs; TIGR01333; cyt_b559_beta; 1.
DR PROSITE; PS00537; CYTOCHROME_B559; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Electron transport; Heme; Iron; Membrane;
KW Metal-binding; Photosynthesis; Photosystem II; Plastid; Reference proteome;
KW Thylakoid; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..44
FT /note="Cytochrome b559 subunit beta"
FT /id="PRO_0000200375"
FT TRANSMEM 19..35
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00643"
FT BINDING 23
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_note="ligand shared with alpha subunit"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00643"
FT CONFLICT 22..30
FT /note="IHGIAVPTI -> TTVLQYQQF (in Ref. 1; CAA46977)"
FT /evidence="ECO:0000305"
FT HELIX 17..39
FT /evidence="ECO:0007829|PDB:6KAC"
SQ SEQUENCE 44 AA; 4981 MW; 314FFE31BDF1C049 CRC64;
MTTKKSAEVL VYPIFTVRWL AIHGIAVPTI FFLGAITAMQ FIQR
